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Carbon dot used as antitumor drug and preparing method and application of carbon dot

An anti-tumor drug and carbon dot technology, which is applied in the field of fluorescent nanomaterials and biomedicine, can solve the problems of not very large release, limited practical application, and low efficiency, and achieves a long cycle time of action, easy promotion, and easy operation. Effect

Active Publication Date: 2018-02-23
LIAONING UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, although arginine reacts with hydrogen peroxide to release NO, the release amount is not very large, and arginine is a biological macromolecule, which can only enter cells through osmosis to play a role in the body, and the efficiency is not high. It also limits its practical application to some extent.

Method used

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  • Carbon dot used as antitumor drug and preparing method and application of carbon dot
  • Carbon dot used as antitumor drug and preparing method and application of carbon dot
  • Carbon dot used as antitumor drug and preparing method and application of carbon dot

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Weigh 11.5mmol L-arginine respectively, add 5.7mmol urea and 5.7mmol ethylenediamine respectively, dissolve in 15mL ultrapure water, after completely dissolving, heat in a microwave oven at 750W for 4 minutes and take it out to obtain L- Arginine urea carbon dots (URCD) and L-arginine ethylenediamine carbon dots (LACD).

[0022] URCD and LACD were each added into 90 mL of PBS buffer solution with pH=6.5 to dissolve. Then take 80 mL of the above-mentioned dissolved liquid and add 100 μL H 2 o 2 . The release of NO in the solution of the two systems after 15 hours of reaction was measured with Griess reagent. The results are shown in Table 5.

[0023] table 5

[0024]

[0025] Table 5 is LACD and URCD respectively with H 2 o 2 Respond to the statistical results of NO release for 15 hours. It can be seen from Table 5 that LACD releases more NO than URCD. After release for 15 hours, the amount of NO released by LACD was 6.5 μM, and the amount of NO releas...

Embodiment 2

[0027] Weigh 2g L-arginine and dissolve them in 15mL ultrapure water with 0.173g, 0.230g, 0.345g, 0.690g, 1.38g ethylenediamine respectively (the ratio of the amount of ethylenediamine to L-arginine 1:4, 1:3, 1:2, 1:1, 2:1), heated in a microwave oven at 750W for 4 minutes and then taken out to obtain L-arginine carbon dots (LACD) with different ratios.

[0028] Dissolve the above five kinds of LACD in 40mL PBS buffer solution with pH=6.5 respectively, then take 20mL each, and add 200μL H 2 o 2 React for 12 hours. Griess reagent was used to measure the release amount of NO after 12 hours of reaction, and the results are shown in Table 6.

[0029] Table 6

[0030] The amount ratio of ethylenediamine to L-arginine substance

[0031] It can be seen from Table 6 that when the ratio of ethylenediamine to L-arginine is 1:2, the amount of NO released is the largest, and the released amount is 28.44 μM.

Embodiment 3

[0032] Example 3 L-arginine carbon dots

[0033] Weigh 2g (11.5mmol) L-arginine, 0.345g (5.7mmol), dissolve it in 15mL ultrapure water, after completely dissolving, heat it in a microwave oven at 750W for 4 minutes and take it out to obtain L-arginine carbon point (LACD).

[0034] The prepared L-arginine carbon dots (LACD) were scanned by electron microscope, and the results were as follows: figure 1 shown, from figure 1 It can be seen that the average particle size of the prepared L-arginine carbon dots (LACD) is 3 nm, and the distribution is uniform, forming uniformly distributed spherical particles.

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Abstract

The invention relates to a carbon dot used as an antitumor drug and a preparing method and application of the carbon dot. With arginine being a carbon source, arginine is completely dissolved with water, and an appropriate amount of thylenediamine is added and subjected to ultrasonic dissolving and microwave heating to obtain the target product carbon dot. Since cancer cells themselves in an organism contain hydrogen peroxide, the carbon dot can be used for treating cancer. The carbon dot can not only conduct fluorescence imaging, but also has the advantages of being high in biocompatibility,large in NO releasing amount, long in acting circular time, high in drug stability, cheap, easy to obtain and the like.

Description

technical field [0001] The invention belongs to the fields of fluorescent nanomaterials and biomedicine, and in particular relates to a carbon dot which can be used as an antitumor drug and a preparation method and application thereof. Background technique [0002] NO is an extremely unstable biological free radical with small molecule and simple structure. It is a gas at room temperature, slightly soluble in water and fat-soluble. It is widely distributed in various tissues in the living body. It is a new type of biological messenger molecule. It also plays a very important biological role in heart, cerebrovascular regulation, nerve and immune regulation. Therefore, it is generally valued by people. It can quickly diffuse through the biomembrane without any intermediary mechanism, and transmit the information produced by a cell to its surrounding cells. The biological role of NO and its mechanism of action are in the ascendant. Research prospects in . [0003] In modern ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/44A61K9/14A61K31/198A61K47/18A61K49/00A61P35/00
CPCA61K9/145A61K31/198A61K33/44A61K49/0017A61K49/0093
Inventor 刘学程佳敏张国林吴秋华
Owner LIAONING UNIVERSITY
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