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DC targeted nano vaccine applied to immunotherapy of liver cancer and preparation method thereof

A nano-vaccine and immunotherapy technology, which is applied in the field of nano-vaccine immunotherapy for liver cancer and its preparation, can solve the problems of poor solubility, achieve the effect of increasing endocytosis, increasing the effect of immunotherapy, and making the preparation method easy to repeat

Inactive Publication Date: 2018-11-13
INST OF BIOMEDICAL ENG CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, chitosan has poor solubility in water, while ethylene glycol chitosan increases the solubility in water while maintaining the characteristics of chitosan, making it easier to operate in experiments

Method used

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  • DC targeted nano vaccine applied to immunotherapy of liver cancer and preparation method thereof
  • DC targeted nano vaccine applied to immunotherapy of liver cancer and preparation method thereof
  • DC targeted nano vaccine applied to immunotherapy of liver cancer and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Step 1, the preparation of mannose modified ethylene glycol chitosan:

[0029] Dissolve ethylene glycol chitosan (205 mg, molecular weight 50,000) and 4-isosulfuric acid phenyl-alpha-D-mannoside (91 mg) in water. At this time, the structural units of ethylene glycol chitosan and 4- The molar ratio of isosulfuric acid phenyl-alpha-D-mannoside is 1:0.3, after adding water, the concentration of ethylene glycol chitosan is 2mg / ml, stirred and reacted at room temperature for 24h, dialyzed against water, freeze-dried, Obtain mannose-modified ethylene glycol chitosan, and the product obtained is denoted as MAN-GCTS, and its NMR characterization is shown in figure 1 , Depend on figure 1 It can be seen that MAN has been successfully introduced into GCTS.

[0030] Step 2, the preparation of nano vaccine:

[0031] GPC-3 144-152 (1 mg) was dissolved in 1 mL phosphate-buffered saline (PBS, pH = 7.4); the MAN-GCTS (10 mg) prepared in Step 1 was dissolved in 5 ml water, and then a...

Embodiment 2

[0038] Step 1, the preparation of mannose modified ethylene glycol chitosan:

[0039] Dissolve ethylene glycol chitosan (205 mg, molecular weight 5000) and 4-isosulfuric acid phenyl-alpha-D-mannoside (9.1 mg) in water, at this time, the structural units of ethylene glycol chitosan and The molar ratio of 4-isosulfuric acid phenyl-alpha-D-mannoside is 1:0.03, after adding water, the concentration of ethylene glycol chitosan is 0.5mg / ml, stirred and reacted at room temperature for 24h, and dialyzed against water, Freeze-dried to obtain mannose-modified ethylene glycol chitosan, and the product obtained was denoted as MAN-GCTS, and its NMR characterization results were compared with those of figure 1 unanimous. It can be seen that MAN has been successfully introduced into GCTS.

[0040] Step 2, the preparation of nano vaccine:

[0041] GPC-3 144-152 (1.5 mg) was dissolved in 1 mL of phosphate-buffered saline (PBS, pH = 7.4); the MAN-GCTS (10 mg) prepared in Step 1 was dissolve...

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Abstract

The invention relates to a DC targeted nano vaccine applied to immunotherapy of liver cancer and a preparation method thereof, which are characterized in that mannose modified ethylene glycol chitosanis used as a carrier material, a liver cancer specific polypeptide antigen phosphatidylinositol proteoglycan-3144-152 peptide (GPC-3144-152, FVGEFFTDV) is an immunizing antigen, and the nano vaccineis prepared by electrostatic interaction. The nano vaccine can increase the antigen endocytosis quantity of dendritic cell (DC), protect antigen from rapid degradation and clearance, prolong the action time of antigen, and stimulate the body to produce an effective immune response. The raw materials of the nano vaccine are cheap and easily available, the preparation method is simple and easy to repeat, the large-scale processing and production are simple, the immunotherapy effect of liver cancer can be enhanced, and therefore, the DC targeted nano vaccine has good application prospects.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a nano-vaccine used in liver cancer immunotherapy and a preparation method thereof. Background technique [0002] The specific tumor immunotherapy with dendritic cell (DC) as the core of tumor vaccine has received more and more attention. Central link in the immune response. [0003] Antigens enter the body in a free state and will be diluted by body fluids and degraded by enzymes, making the uptake of antigens by DCs extremely low, making it difficult to induce effective immune responses in the body. Nano-vaccine can protect the antigen from rapid degradation and clearance. By designing the nano-particle structure, it can also realize the targeted delivery and controlled release of the antigen, thereby increasing the uptake of the antigen by DC and prolonging the action time of the antigen, which is conducive to stimulating the body. produce an effective immune response....

Claims

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Application Information

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IPC IPC(8): A61K39/00A61K47/36A61P35/00
CPCA61K39/0011A61K47/36A61P35/00
Inventor 张闯年孙洪范孔德领
Owner INST OF BIOMEDICAL ENG CHINESE ACAD OF MEDICAL SCI
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