49results about How to "Great potential for clinical application" patented technology

The invention belongs to the field of medicine preparations and particularly relates to a natural nanoparticle-medicine composition for resisting Alzheimer's disease (AD) and a preparation method, property evaluation and application thereof. By recombining extracted natural lipoprotein nanoparticles and AD treatment medicine to prepare the natural nanoparticle-medicine composition, combined treatment of AD is achieved. The combined treatment is high in homologous bionic performance and safety, high in medicine loading capacity, efficient in brain targeting, high in amyloid protein affinity, capable of achieving targeting removing, mild in preparation conditions, simple in preparation process and easy in industry expanding. The natural nanoparticle-medicine composition is administrated through intravenous injection, oral taking, nasal delivery and the like, a new thought and technology is provided for the research and development of new AD medicine, and the natural nanoparticle-medicinecomposition has an important research value and promising clinical research prospect.

The invention discloses a preparation method and application of a heat-induced irreversible composite hydrogel with antibacterial and wound healing functions. After the gel is dissolved in a silver-loaded graphene solution, it is sprayed on the wound surface and stimulated by the temperature of the wound surface. An irreversible hydrogel is formed in response. The temperature-sensitive hydrogel provided by the present invention has a simple preparation method, and has the functions of antibacterial and wound healing at the same time. It is used as a wound dressing, has obvious effects, and has great potential for clinical application.

The invention discloses a method for using a Pickering emulsion method for producing GelMA macropore hydrogel and application. The method comprises the steps of dissolving GelMA and PEG-4SH in PBS buffer liquid, and stirring a mixture; adding MgO nano-particles after fully dissolving the GelMA and the PEG-4SH, stirring a mixture, and conducting ultrasonic treatment on the mixture; adding dodecaneand a photoinitiator, and using a high-speed homogenizer for intensively stirring a mixture to obtain stable Pickering emulsion; and making the emulsion subjected to UV illumination to form gel, and obtaining the GelMA macropore hydrogel by using ethyl alcohol and water for full wash dialysis. The produced GelMA macropore hydrogel has the advantage of the macropore structure, can promote transportation of nutrient substances, discharging of metabolic products and cell communication, entraps a component containing Mg, has effect of promoting adhesion and proliferation of bone marrow mesenchymalstem cells and promoting osteogenic differentiation, has excellent biocompatibility, can be used as a bone tissue engineering scaffold with excellent performance, and has large clinical application potential in the field of bone repair.

The invention discloses a DNA mutation test method in use of nano-Au, relates to a DNA mutation test method, and provides a DNA mutation test method that uses a nano-Au probe. The DNA mutation test method leads wild single-stranded DNA, a probe that is completely complementary with a wild single-stranded DNA sequence and the nano-Au to be mixed, statically placed, added with a sodium chloride solution, and then statically placed, thus obtaining an architecture 1; the DNA mutation test method further leads mutant single-stranded DNA, the probe that is completely complementary with the wild single-stranded DNA sequence and the nano-Au to be mixed, statically placed, added with the sodium chloride solution, and then statically placed, thus obtaining an architecture 2; the sodium chloride solution is added into and evenly mixed with the architecture 1, the ultraviolet-visible absorption spectrum detection is implemented, and an absorbance value obtained at a 520nm position is taken as an absorbance value 1; the sodium chloride solution is added into and evenly mixed with the architecture 2, the ultraviolet-visible absorption spectrum detection is implemented, and an absorbance value obtained at a 520nm position is taken as an absorbance value 2; when the absorbance values 2 and 1 have marked difference, mutation is determined to exist. The DNA mutation test method does not need any modification of the DNA and the nano-Au, thus simplifying sample processing.

The present invention relates to weakly basic derivatives of cabazitaxel and preparations thereof, in particular to the synthesis of weakly basic derivatives of cabazitaxel, liposome preparations containing the derivatives and their application in drug delivery systems, belonging to medical technology field. The weakly basic derivative of cabazitaxel is connected with a weakly basic intermediate through an ester bond, and the ester bond can be broken by the action of esterase in the body to release the active drug. Its structural general formula is as follows: wherein, linking group is C 1 -C 4 Alkyl, C 3 -C 6 Cycloalkyl or phenyl; [N] is N-methylpiperazinyl, piperidinyl, 4-(1-piperidinyl) piperidinyl, morpholinyl, tetrahydropyrrolyl or other tertiary amine structures. The weakly basic derivatives of cabazitaxel of the present invention can be prepared into liposome preparations. The liposome preparation has the characteristics of high drug loading, high encapsulation efficiency, good stability and the like. After injection, it can greatly increase the circulation time of the drug in the body, increase the accumulation of the drug in the tumor site, and improve the anti-tumor effect and tolerance dose. .

The invention discloses a dual-ring hairpin probe mediate label-free strand displacement amplification method for detecting bleomycin. The method includes the steps that when bleomycin exists, a dual-ring hairpin probe fractures at a recognition site, and a triggering sequence is released; the triggering sequence and a ring part of a signal probe are combined and subjected to a strand displacement amplification reaction under the effect of a polymerase and a nicking enzyme, and finally a great number of G-four-chain sequences are generated. At the same time, a primer chain extends to open the signal probe, and therefore the G-four-chain sequences packaged in the neck of the signal probe can be exposed. Finally, NMM molecules are bound to the G-four-chain sequences to generate fluorescence signals, and bleomycin is quantified through the detected fluorescence signals. As the triggering sequence is designed at the neck of the dual-ring hairpin probe, background signals of a detection system are reduced; by combining bleomycin cutting and the strand displacement amplification reaction, bleomycin can be detected sensitively, and the detection limit is 0.34 nm. The method has the advantages of being easy to operate, free of labeling and good in specificity.

The invention belongs to the field of pharmaceutical preparations, and discloses a chitosan lipoprotein intranasal administration nanocomposite, comprising lipoprotein nanoparticles, chitosan or / and chitosan derivatives having the effect of promoting absorption of nasal mucosa. The invention also discloses a preparation method of chitosan lipoprotein intranasal administration nanocomposite, comprising: self-assembling chitosan or / and chitosan derivatives and lipoprotein nanoparticles through dynamic force to form a good membrane permeability nasal preparations. The preparation condition of the chitosan lipoprotein intranasal administration nanocomposite is mild, the process is simple, and the industrialized production is easy. The system is safe, non-toxic, and has good biodegradability, which can improve the absorption of lipoprotein drugs in the nasal mucosa and ensure high-efficiency brain targeting. The dosage form is administered by nasal drops, sprays, etc., and is easy to operate, convenient for patients who take the medicine for a long time, and has a good clinical application prospect in the treatment of central nervous system diseases.

The application provides a capsule endoscope system, including: a capsule endoscope and an external locator; the capsule endoscope includes a capsule endoscope body, the capsule endoscope body includes a capsule endoscope shell and a capsule endoscope magnet, and the capsule endoscope magnet is set Inside the shell of the capsule endoscope; the external locator includes an external magnet; the capsule endoscope magnet and the external magnet are respectively arranged on the inside and outside of the site to be tested, and are fixed on the site to be tested by mutual attraction, or the external magnet moves to make the inside of the capsule endoscopic. The mirror magnet moves at the site to be tested; the capsule endoscope also includes a connecting tube, one end of the connecting tube is connected to the outer surface of the capsule endoscope shell, and the other end of the connecting tube is suspended in the air; the connecting tube is used for washing the capsule endoscope and for recovering the capsule Endoscopy. By adopting the capsule endoscope system provided by the present invention, doctors can detect blunt abdominal injuries in real time, quickly, directly and accurately, with simple structure, easy operation and great clinical application potential.

The invention relates to a protein-type nanoparticle for multispecific antibody delivery and its application and preparation method. The protein-type nanoparticles include polyester and a protein having a hydrophobic domain, and the hydrophobic domain of the protein is combined with the polyester through hydrophobic interactions; the protein is at least one of albumin, globulin and cell wall protein A sort of. The protein-type nanoparticles of the present invention have excellent stability and biocompatibility, and can prepare a multispecific antibody delivery platform (αFc-NP) by bonding anti-IgG-Fc antibodies or anti-IgG-Fc antibody fragments, αFc-NP It can stably, quickly and easily bind to multiple specific antibodies through antigen-antibody interaction and enhance the therapeutic effect of specific antibodies.