126 results about "Microinjections" patented technology

The present invention generally relates to devices and methods for the preservation of cells using drying, freezing, and other related techniques. In one set of embodiments, the invention allows for the preservation of cells in a dried state. In another set of embodiments, the invention allows for the preservation of cells within a glass or other non-viscous, non-frozen media. In some embodiments, the invention allows for the preservation of cells at temperatures below the freezing point of water, and in some cases at cryogenic temperatures, without inducing ice formation. The cells, in certain embodiments, may be preserved in the presence of intracellular and / or extracellular carbohydrates (which may be the same or different), for example, trehalose and sucrose. Carbohydrates may be transported intracellularly by any suitable technique, for example, using microinjection, or through non-microinjected methods such as through pore-forming proteins, electroporation, heat shock, etc. In certain instances, the glass transition temperature of the cells may be raised, e.g., by transporting a carbohydrate intracellularly. In some cases, the cells may be dried and / or stored, for example, in a substantially moisture-saturated environment or a desiccating environment. The cells may also be stored in a vacuum or a partial vacuum. The cells may be protected from oxygen, moisture, and / or light during storage. In certain cases, an inhibitor, such as a cell death inhibitor, a protease inhibitor, an apoptosis inhibitor, and / or an oxidative stress inhibitor may be used during preservation of the cells. The cells may be stored for any length of time, then recovered to a viable state, e.g., through rehydration, for further use.

The invention discloses a method for synthesizing and secreting black widow spider dragline silk protein 1 through a bombyx mori silk gland bioreactor. The method includes the steps that firstly, pBac[3xP3-DsRed]-MaSp1 plasmids serving as a carrier for bombyx mori to synthesize and secrete black widow spider dragline silk protein 1 are established; then, plasmids and assistant plasmids are introduced into bombyx mori zygotes through microinjection, red fluorescence protein genes and black widow spider dragline silk protein 1 genes are introduced into bombyx mori genomes through the transposition characteristic of piggyBac transposons, stable inheritance and expression are performed, transgenic bombyx mori is obtained, mori moth selfing is performed to achieve homozygosis of black widow spider dragline silk protein 1 genes, and transgenic bombyx mori secreting black widow spider dragline silk protein 1 is bred. According to the method, transgenic bombyx mori is screened through fluorescence marker genes, and black widow spider dragline silk protein 1 is specifically synthesized and secreted through bombyx mori silk gland cells; the method is used for development and utilization of spider silk and can also be used for improving the mechanical performance of silk.

A method has been developed to produce stable cell-matrix microspheres with up to 100% encapsulation efficiency and high cell viability, using matrix or biomaterial systems with poor shape and mechanical stability for applications including cell therapeutics via microinjection or surgical implantation, 3D culture for in vitro expansion without repeated cell splitting using enzymatic digestion or mechanical dissociation and for enhanced production of therapeutic biomolecules, and in vitro modeling for morphogenesis studies. The modified droplet generation method is simple and scalable and enables the production of cell-matrix microspheres when the matrix or biomaterial system used has low concentration, with slow phase transition, with poor shape and mechanical stability.