The invention relates to a primer composition for detecting
staphylococcus aureus, a
reagent kit and a dual-
signal-path detection method thereof. The method comprises the steps that
nucleic acid extraction is performed on a sample to be detected; an isothermal amplification reaction is performed, wherein the isothermal amplification reaction is performed in a
reaction system containing a color indicator,
DNA embedded dye,
DNA of the sample to be detected and amplification primers thereof; result interpretation is performed, wherein according to
color changes of the indicator obtained after amplification is ended, direct
naked eye interpretation is performed and / or a special instrument is combined for detecting a
nucleic acid amplification curve for interpretation. In the
reaction system, the macroscopic indicator is added, and the problem that an operator cannot recognize whether samples are added successfully or nor is avoided; according to the dual-
signal-path detection method the
DNA embedded dye is combined with the matched instrument for detecting the amplification curve, and the
macroscopic observation result of the indicator is obtained, one
signal path can be selected for result determination according to the requirement for application scenarios, and the method is easier, more convenient to implement, rapid, easy to implement and applicable to field operation in actual application.