150 results about "Lincomycin-B" patented technology

The invention discloses a processing method of lincomycin fungus dregs, effectively settling the problem of degradation and clearance of residual lincomycin inside the fungus dregs and enabling the regeneration and reuse of wastes. The processing method of lincomycin fungus dregs can be realized through the following steps: the lincomycin fungus dregs, EM bacteria stock solution, accessories and water are used as materials; wherein, the total weight ratio of the lincomycin and the accessories is 1 to 3:1; the accessories comprise bran, brown sugar, sodium bicarbonate, table salt and peanut shell with the weight ratio of 50:6:3:16:125; wherein, the bran and the peanut shell are pulverized, then the sodium bicarbonate and table salt are added to the lincomycin fungus dregs in series, 40 to 50ml EM bacteria stock solution is measured for 1kg lincomycin fungus dregs added with brown sugar and diluted by water to 250ml, forming EM bacteria stock diffusion solution; then the EM bacteria stock diffusion solution is added to the mixture and mixed uniformly, added with water, and stored in a vessel for fermentation. The processing method of lincomycin fungus dregs has the advantages of simple method, waste reuse, and great economic and social benefits.

The invention provides a pidotimod-containing pharmaceutical composition and a preparation method thereof. The pidotimod-containing pharmaceutical composition consists of pidotimod and any antibacterial medicament. The antibacterial medicament may be any of beta-lactams (including as penicillins, cephems and unclassical beta-lactams), aminoglycosides, polymyxins, macrolides, lincomycins, norvancomycins, tetracyclines, chloromycetins, artificial antibacterial medicaments (including sulfonamides, quinolones and furofurans), cholesterols and antiviral medicaments. The unit dosage of the pidotimod is 0.1 to 2 grams, preferably 0.4 to 0.8 gram. The dosage of the antibacterial medicament is determined according to the dosage range of the antibacterial medicament. The composition of the pidotimod and the antibacterial medicament can exogenously and directly kill pathogenic bacteria through the antibacterial medicament and mobilize endogenous anti-infection immunity through an immunomodulator to improve anti-inflection effect, and has excellent synergy.

The Lincomycin extracting process includes the steps of mixing benzyl alcohol and n-octyl alcohol in the volume ratio of 1.5-9 in room temperature, extracting fermented and filtered Lincomycin liquid with the binary extractant, back extraction with water solution of hydrochloric acid or sulfuric acid in 0.01-1 M concentration, and further refining of the back extracted liquid to obtain Lincomycin. The binary extractant of the present invention has low toxicity, less environmental pollution, little damage to persons, easy-to-control room temperature operation and improved operation environment. The present invention may be used widely in biomedicine preparing field.

The invention discloses a method of increasing the lincomycin output by modifying the gene of streptomyces lincolnensis SLCG_2919. According to the method, a TetR family transcription regulation geneSLCG_2919 is deleted from streptomyces lincolnensis by a gene engineering process to obtain an engineering strain with a high yield of lincomycin; the obtained strain can produce lincomycin through fermentation, the output is largely increased, and novel technical support is provided for increasing the lincomycin output in industrial production.

The invention relates to a culture medium and a cultivation method for producing lincomycin by fermenting streptomyces lincolnensis. By the use of a seed culture medium formula, a fermentation culture medium formula and a control process which are provided by the invention, the fermentation unit for large-scale production of lincomycin can be increased, and the fermentation cost can be reduced; furthermore, the environmental influence on raw and auxiliary materials can be reduced to the maximum extent; sufficient supply of the materials is guaranteed, and efficient production of lincomycin is realized.

The invention discloses a detection method for lincomycin and a special test paper therefor. The test paper includes a sample adsorption pad, a conjugate release pad, a reaction membrane, and a water absorption pad connected in order. The conjugate release pad is coated with a colloidal gold labeled lincomycin prime specific antibody, which is a lincomycin polyclonal antibody or a lincomycin monoclonal antibody. The reaction membrane comprises a detection zone and a quality control zone, wherein the detection zone is coated with a conjugate of hapten and carrier protein, and the quality control zone is coated with an antiantibody. The method for detection of lincomycin by the test paper provided by the invention is simple, fast, visualized and accurate, and has the advantages of wide application range, low cost, and easy popularization and application.

The invention discloses a dilute solution used for preserving racoon dog seminal fluid at normal temperature, and a semen collection method. The dilute solution comprises following ingredients: 36 to 38g of glucose, 1.20 to 1.30g of sodium bicarbonate, 1.20 to 1.30g of ethylene diamine tetraacetic acid, 5.50 to 6.30g of sodium citrate, 0.65 to 0.85g of potassium chloride, 0.50 to 3.05g of bovine serum albumin, 0.05 to 0.30g of acetylcysteine, 0.50 to 1.20g of penicillin, 0.30 to 1.00g of streptomycin, 0.05 to 1.50g of lincomycin and 0.01 to 0.05g of spectinomycin; and a mixture of the ingredients above is diluted to 1000ml by adding triple-distilled water so as to obtain the dilute solution. Key steps of the semen collection method are that: female racoon dogs in heat are used as dummy teaser; conditioned reflex of male racoon dogs to artificial semen collection is formed by training; the training is repeated for 3 to 4 times until conditioned reflex is formed; and then the female racoon dogs are replaced by a female racoon dog-simulated dummy teaser which is filled with urine of female racoon dogs in heat so as to collect male racoon dog semen. The dilute solution and the semen collection method can be used for collecting racoon dog semen, diluting for preservation, realizing artificial insemination and obtaining offspring; and are capable of promoting the popularization of racoon dog artificial insemination technologies, and increasing economic benefits of racoon dog industry.

The invention belongs to the technical field of pharmaceutical products and provides a lincomycin purification method. The lincomycin purification method is mainly characterized in that alkaline water is taken as a mobile phase, a resin column is taken as a fixed phase, and adsorption, washing, resolution, vacuum concentration, crystallization, drying and other steps are performed for treatment. According to the lincomycin purification method provided by the invention, the content of a component B in lincomycin can be effectively reduced, and the lincomycin purification method has the advantages of low cost, environmental friendliness and no pollution.

The invention provides a method for preparing an organic fertilizer from lincomycin fungi residues, and relates to harmless treatment and recovery of the lincomycin fungi residues. The method provided by the invention aims at realizing the harmless treatment and resource utilization of the lincomycin fungi residues. The method concretely comprises the following steps of collecting fresh lincomycin fungi residues; regulating the moisture content and the pH value; adding potassium peroxydisulfate and zinc acetate for treatment; performing centrifugation and precipitate collection; manufacturing the organic fertilizer. The lincomycin residues are damaged and removed; antibiotic drug resistant bacteria in the fungi residues are inactivated. The method has the advantages that the process is simple; the operation is easy. The potassium peroxydisulfate is used; the removal rate on the lincomycin is high and reaches 99.9 percent; the treatment cost is low; the period is short; the operation can be completed in half an hour. The fungi residue organic fertilizer does not contain antibiotic drug resistant bacteria; bacterium drug resistance cannot be caused in the soil; the use safety is high.