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Detection method for lincomycin and special test paper therefor

A lincomycin and test strip technology, applied in the field of lincomycin detection, can solve the problems of increased drug resistance of Gram-positive bacteria, unsuitable rapid detection, human health hazards, etc., and achieves short detection time and low cost. , store simple effects

Active Publication Date: 2014-08-20
BEIJING KWINBON BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Lincomycin has antibacterial and anti-inflammatory effects. Criminals often add it to acne-removing cosmetics without authorization to achieve the purpose of quickly and effectively eliminating acne, acne, and acne. However, long-term use will irritate the skin and cause renal dysfunction and leather. Increased drug resistance of Lambert-positive bacteria, causing serious harm to human health
[0003] At present, the testing methods for illegally adding lincomycin in acne-removing cosmetics are mostly instrumental analysis methods, which are costly and time-consuming, and require a variety of chemical reagents and professional testing personnel, which are not suitable for rapid testing under simple conditions. detection

Method used

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  • Detection method for lincomycin and special test paper therefor
  • Detection method for lincomycin and special test paper therefor
  • Detection method for lincomycin and special test paper therefor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1, detect the composition of the test paper of lincomycin

[0035] 1. Test paper without protective film or test paper card ( figure 1 ):

[0036] The test paper is composed of a sample absorbent pad 1, a conjugate release pad 2, a reaction membrane 3, a water absorbent pad 4 and a bottom plate 7;

[0037] The sample absorbent pad, the conjugate release pad, the reaction film and the water absorbent pad are pasted on the bottom plate in sequence, and 1 / 3 of the conjugate release pad is covered by the sample absorbent pad from its beginning, and the conjugate release pad The end of the reaction membrane is connected to the beginning of the reaction membrane, the end of the reaction membrane is connected to the beginning of the absorbent pad, the beginning of the sample absorbent pad is aligned with the beginning of the base plate, and the end of the absorbent pad is aligned with the end of the base plate;

[0038] The conjugate release pad is coated with co...

Embodiment 2

[0062] The preparation method of kit described in embodiment 2, embodiment 1

[0063] 1. Preparation of kits

[0064] The preparation method of the kit mainly includes the following steps:

[0065] 1) Prepare a conjugate release pad coated with lincomycin monoclonal antibody-colloidal gold label;

[0066] 2) Prepare a reaction membrane containing a detection zone coated with lincomycin hapten-carrier protein conjugate and a quality control zone coated with goat anti-mouse IgG;

[0067] 3) Assemble the sample absorbent pad, the above-mentioned conjugate release pad, the reaction membrane, the absorbent pad and the bottom plate into a test paper.

[0068] 4) Paste the protective film on both ends of the assembled test paper; cut the assembled test paper into 3mm wide strips by machine; or cut the assembled test paper into 3mm wide strips by machine, and put them in a special plastic box.

[0069] The following step-by-step detailed description:

[0070] (1) Preparation of ea...

Embodiment 3

[0118] Embodiment 3, the application of test paper

[0119] One, detect lincomycin with test paper described in Example 1

[0120] The test paper of the invention can detect solid cosmetic samples and liquid cosmetic samples.

[0121] 1. Sample handling:

[0122] Solid cosmetic samples, liquid cosmetic samples: take 0.2g or 0.2ml cosmetic samples, shake and mix them with 4ml extract solution, then take 200μl mixed solution into 600μl extract solution and mix well, then take samples for detection; the extract solution contains 0.5% spit Phosphate buffered saline with Wen-20 active agent (percentages are by volume).

[0123] 2. Detection method and judgment

[0124] After dropping the sample solution to be tested into the sample absorbent pad in the test paper in this kit, watch the test result within 5-8 minutes.

[0125] When the concentration of lincomycin in the sample is higher than the minimum detection limit of the test paper, the colloidal gold antibody binds to linc...

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Abstract

The invention discloses a detection method for lincomycin and a special test paper therefor. The test paper includes a sample adsorption pad, a conjugate release pad, a reaction membrane, and a water absorption pad connected in order. The conjugate release pad is coated with a colloidal gold labeled lincomycin prime specific antibody, which is a lincomycin polyclonal antibody or a lincomycin monoclonal antibody. The reaction membrane comprises a detection zone and a quality control zone, wherein the detection zone is coated with a conjugate of hapten and carrier protein, and the quality control zone is coated with an antiantibody. The method for detection of lincomycin by the test paper provided by the invention is simple, fast, visualized and accurate, and has the advantages of wide application range, low cost, and easy popularization and application.

Description

technical field [0001] The invention relates to a method for detecting lincomycin and a special test paper thereof. Background technique [0002] Lincomycin has antibacterial and anti-inflammatory effects. Criminals often add it to acne-removing cosmetics without authorization, so as to achieve the purpose of quickly and effectively eliminating acne, acne, and acne. However, long-term use will irritate the skin and cause kidney dysfunction and leather Increased drug resistance of Lambert-positive bacteria has caused serious harm to human health. [0003] At present, the testing methods for illegally adding lincomycin in acne-removing cosmetics are mostly instrumental analysis methods, which are costly and time-consuming, and require a variety of chemical reagents and professional testing personnel, which are not suitable for rapid testing under simple conditions. detection. Therefore, it is necessary to establish a simple and easy detection method for lincomycin in respons...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/577
CPCG01N33/558G01N33/577
Inventor 何方洋万宇平罗晓琴孙震冯静田甜何丽霞余厚美徐念琴
Owner BEIJING KWINBON BIOTECH
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