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Method used for separating and purifying lincomycin with molecular imprinting polymers

A technology of lincomycin and molecular imprinting, which is applied in the direction of preparation of sugar derivatives, sugar derivatives, sugar derivatives, etc., can solve the problems of high yield of lincomycin, complex treatment process of lincomycin aqueous solution, extraction, Solving problems such as cumbersome stripping process to achieve good separation effect

Inactive Publication Date: 2015-11-11
WUHAN INSTITUTE OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The lincomycin B component that this method obtains is less than 1%, and the purity is high, and the disadvantage is that the lincomycin aqueous solution treatment process is comparatively complicated
This method has a high yield of lincomycin, which can reach more than 96%, and can effectively reduce the COD of production wastewater. The disadvantage is that the filtration operation needs to repeatedly adjust the pH value, and the extraction and back-extraction processes are relatively cumbersome.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0019] The preparation process of the lincomycin molecularly imprinted polymer in the scheme is as follows:

[0020] 1) Mix styrene monomer with absolute ethanol, and add polyvinylpyrrolidone and azobisisobutyronitrile, wherein the volume ratio of styrene and ethanol is controlled to be 1:(1~1.4), azobisisobutyronitrile The mass ratio to polyvinylpyrrolidone is 1:(5~7.5); under the condition of isolating air, the reaction is stirred at 60~70℃ for 20~24h, and the finished polystyrene microspheres are obtained by separation and drying;

[0021] 2) Take step 1) polystyrene microspheres and sodium dodecyl sulfate and evenly disperse them in ultrapure water, stir and swell, wherein the mass ratio of polystyrene microspheres to sodium dodecyl sulfate is (4-5 ): 1; add azobisisobutyronitrile, dibutyl phthalate and toluene with stirring and swelling for 20 to 24h, wherein the mass ratio of azobisisobutyronitrile, dibutyl phthalate and toluene is 1 :(5.2~6.3):(8.7~9.6);

[0022] 3) T...

Embodiment 1

[0032] Take a certain amount of lincomycin fermentation broth, adjust its pH value to 10.2 with an aqueous sodium hydroxide solution, and repeatedly extract twice with the same volume of chloroform to obtain the extract to be adsorbed;

[0033] Mix styrene monomer with absolute ethanol, add dispersant polyvinylpyrrolidone and initiator azobisisobutyronitrile, ultrasonically disperse on ultrasonic cell disintegrator, make it evenly mixed, add into four-necked flask, heat for polymerization , the obtained polymer is centrifuged, washed and dried to finally obtain finished polystyrene microspheres. Take the dried polystyrene microspheres and dispersant sodium dodecyl sulfate, add them into ultrapure water, ultrasonically disperse them on an ultrasonic cell disintegrator to make them evenly mixed and then add them into a four-necked flask; take the initiator azo Diisobutyronitrile, swelling agent dibutyl phthalate and porogen toluene were added to ultrapure water, and ultrasonical...

Embodiment 2

[0038] Take a certain amount of lincomycin fermentation broth, adjust its pH value to 10.5 with an aqueous sodium hydroxide solution, and repeatedly extract it with the same volume of chloroform for 3 times to obtain the extract to be adsorbed;

[0039]Mix styrene monomer with absolute ethanol, add dispersant polyvinylpyrrolidone and initiator azobisisobutyronitrile, ultrasonically disperse on ultrasonic cell disintegrator, make it evenly mixed, add into four-necked flask, heat for polymerization , the obtained polymer is centrifuged, washed and dried to finally obtain finished polystyrene microspheres. Take the dried polystyrene microspheres and dispersant sodium dodecyl sulfate, add them into ultrapure water, ultrasonically disperse them on an ultrasonic cell disintegrator to make them evenly mixed and then add them into a four-necked flask; take the initiator azo Diisobutyronitrile, swelling agent dibutyl phthalate and porogen toluene were added to ultrapure water, and ultr...

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Abstract

The invention discloses a method used for separating and purifying lincomycin with molecular imprinting polymers. The method comprises following steps: a lincomycin fermentation liquor is filtered, pH value is adjusted to 10 to 11 with a sodium hydroxide aqueous solution, and an extract liquor is obtained via 2 to 3 times of extraction with chloroform; the extract liquor is pumped into an adsorption column filled with the lincomycin molecular imprinted polymers, and adsorption separation is carried out until absorption column saturation is achieved; the absorption column is subjected to elution with a methyl alcohol-acetic acid mixed solution at 25 to 40 DEG C at a flow rate ranging from 3 to 5ml / min; an obtained eluent is subjected to vacuum concentration at 60 to 70 DEG C until the volume of the eluent is reduced to be one third to one half of an original volume, pH value is adjusted to be 0.5 to 2.0, and an obtained product is cooled to 0 to 10 DEG C, and is subjected to crystallizing and drying so as to obtain lincomycin. Purity of obtained lincomycin is 94.6%, yield is 81.93%, separation effect is excellent, and the method is suitable for industrial separation and extraction of lincomycin.

Description

technical field [0001] The invention relates to the field of drug extraction and separation, in particular to a process method for separating and purifying lincomycin by using molecularly imprinted polymers. Background technique [0002] The chemical name of lincomycin is 6-(1-methyl-trans-propyl-L-2-pyrrolidinecarboxamido)-1-thio-6,8-dideoxy-D-erythro-a-D-half Lactocin pyranoside is a class of antibiotics obtained from the culture medium of Streptomyces sp. It can act on the 50S subunit of the ribosome of sensitive bacteria to prevent the formation of peptide bonds, thereby inhibiting the protein synthesis of bacteria and achieving the purpose of antibacterial. It is suitable for the treatment of various infections caused by Gram-positive bacteria, such as pneumonia caused by Streptococcus pneumoniae, Staphylococcus aureus, empyema, sepsis, skin and soft tissue infections, abdominal infections, bone and joint infections, gynecological infections, etc. Lincomycin has the c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/16C07H1/06C08F212/08C08F220/06C08F222/14C08F2/44C08J9/26
Inventor 张佑红钟季良李卫朋
Owner WUHAN INSTITUTE OF TECHNOLOGY
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