The invention relates to a detection card for rapidly detecting
canine distemper virus antigens and a preparation method of the detection card. The method includes the following specific steps of: (1)the isolation, culture and purification of
canine distemper viruses; (2) the preparation of paired
monoclonal antibodies against the
canine distemper viruses, wherein the preparation involves animmunization procedures,
cell fusion, hybridoma screening and
cloning,
antibody preparation and purification; and (3) the preparation method of the
colloidal gold detection card for rapidly detecting the canine distemper viruses, wherein the preparation method involves sample pad treatment, film spraying, and C, T line determination, to-be-tested
sample processing and
test card performance measurement.With the preparation method of the detection card of the invention adopted, canine distemper viruses in China can be quickly, sensitively and accurately detected. According to the prior art, paired
monoclonal antibodies for developing
colloidal gold detection cards for detecting canine distemper viruses in China are made of imported raw materials; the imported raw materials of the
monoclonal antibodies are prepared from current viruses isolated by a certain country. With the preparation method of the invention adopted, the conditions in the prior art can be avoided, and a phenomenon that missed detection caused by
virus variation due to long distances between areas during a detection process can be avoided.