Trivalent inactivated vaccine of porcine reproductive and respiratory syndrome virus, porcine circovirus type 2, and porcine pseudorabies virus and preparation method thereof

A technology for respiratory syndrome and porcine circovirus, applied in antiviral agents, viral antigen components, pharmaceutical formulations, etc., can solve problems such as shortages, improve safety, improve safety and immune efficacy, and reduce epidemic prevention costs Effect

Inactive Publication Date: 2013-03-20
PU LIKE BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, there has been a lack of multivalent vaccines for PRRSV, PRV and PCV2 on the market.

Method used

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  • Trivalent inactivated vaccine of porcine reproductive and respiratory syndrome virus, porcine circovirus type 2, and porcine pseudorabies virus and preparation method thereof
  • Trivalent inactivated vaccine of porcine reproductive and respiratory syndrome virus, porcine circovirus type 2, and porcine pseudorabies virus and preparation method thereof
  • Trivalent inactivated vaccine of porcine reproductive and respiratory syndrome virus, porcine circovirus type 2, and porcine pseudorabies virus and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1: Preparation and inspection of porcine reproductive and respiratory syndrome-porcine circovirus disease type 2-porcine pseudorabies triple inactivated vaccine

[0032] 1 Materials and methods:

[0033] 1.1 Source of virus strain:

[0034] Selected strain is porcine reproductive and respiratory syndrome virus NVDC-JXA1 strain (referring to Chinese patent CN100439494C), porcine circovirus type 2 is SH strain (referring to Chinese patent CN101240264A), pseudorabies strain is Fa strain (purchased from Chinese Veterinary Medicine Drug Control Office),

[0035] 1.2 Preparation and inspection of virus liquid for seedling production

[0036] (1) Preparation and inspection of PRRSV virus liquid

[0037] Inoculate Marc-145 into a culture solution with a pH value of 7.0-7.4 and a polyester fiber carrier tank, mix Marc-145 cells with polyester fiber evenly, start the attachment procedure for 4 hours, and make Marc-145 cells adhere to the on polyester fiber. Switch...

Embodiment 2

[0113] Embodiment 2, porcine reproductive and respiratory syndrome-porcine circovirus disease type 2-porcine pseudorabies triple inactivated vaccine and single vaccine (porcine reproductive and respiratory syndrome inactivated vaccine or porcine circovirus type 2 inactivated vaccine or Comparison of immune effects of porcine pseudorabies inactivated vaccine).

[0114] 1. Materials

[0115] Porcine reproductive and respiratory syndrome-porcine circovirus disease type 2-porcine pseudorabies triple inactivated vaccine, the laboratory product in Example 1, batch number: 1101; porcine reproductive and respiratory syndrome inactivated vaccine (NVDC-JXA1 strain ), source: Luoyang Pulaike Bioengineering Co., Ltd., batch number: 110213; porcine circovirus type 2 inactivated vaccine (SH strain), source: Luoyang Pulaike Biological Engineering Co., Ltd., batch number: 110115; porcine pseudorabies Live vaccine (Ea strain), source: Wuhan Keqian Biological Products Co., Ltd., batch number: ...

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Abstract

The present invention provides a trivalent inactivated vaccine of porcine reproductive and respiratory syndrome virus, porcine circovirus type 2, and porcine pseudorabies virus. Before inactivation, the contents of the three viruses are respectively greater than 10<8.5>TCID[50]/ml 10<6.0>TCID[50] /ml and 10<8.0>TCID[50]/ml; And after the inactivation, the volume ratio of the three antigens is 1:1:1. According to the present invention, via a large number of detailed tests, the contents and ratio of the three viral antigens are selected; and immune effects are measured in a large number of experimental animals and swine themselves, to ensure that the phenomenon of immune interference does not occur among the various immune components in the multivalent vaccine. Compared with the existing three individual vaccines of the same three virus, wherein three injections are need to prevent the three diseases caused by the three virus by using the three individual vaccines, the trivalent inactivated vaccine of the present invention is economical and practical, and simplifies the immunization procedure, and reduces the cost of epidemic prevention. The present invention realizes preparation and application of multivalent inactivated vaccine of porcine reproductive and respiratory syndrome virus, porcine circovirus type 2, and porcine pseudorabies virus, which has not been achieved for all the time in the field.

Description

technical field [0001] The invention relates to a polyvalent inactivated vaccine for animals and a preparation method thereof, in particular to a triple inactivated vaccine of porcine reproductive and respiratory syndrome, porcine circovirus disease and porcine pseudorabies and a preparation method thereof. Background technique [0002] Porcine reproductive and respiratory syndrome (PRRS) is caused by porcine reproductive and respiratory syndrome virus, which is characterized by reproductive disorders such as abortion, stillbirth, mummified fetus, and weak piglets in pregnant sows, as well as respiratory symptoms in piglets. Highly contagious infectious disease characterized by high death rate. In 1996, PRRSV was isolated from PRRS seropositive pigs in my country, which confirmed the prevalence of this disease in my country, and it was also listed as a second-class infectious disease in China. Because PRRS is highly contagious and can spread both vertically and horizontally...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/295A61P31/14A61P31/20A61P31/22A61K39/12A61K39/245
Inventor 张许科孙进忠白朝勇
Owner PU LIKE BIO ENG
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