Preparation method of genetic engineering vaccine for preventing staphylococcus caprae mastitis application

The technology of genetic engineering vaccine and staphylococcus is applied in the field of genetic engineering vaccine preparation for preventing goat staphylococcus mastitis. Convenience, promote mucosal immunity, good effect of cross-protective reaction

Active Publication Date: 2013-01-02
NORTHWEST A & F UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with the development of large-scale intensive farming, artificially attenuated strains have potentials such as homologous recombination and self-virulence reversion, and inactivated vaccines also have shortcomings such as large doses and short immunity periods. The safety and immune protection, it is extremely important to develop efficient and cheap new vaccines

Method used

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  • Preparation method of genetic engineering vaccine for preventing staphylococcus caprae mastitis application
  • Preparation method of genetic engineering vaccine for preventing staphylococcus caprae mastitis application
  • Preparation method of genetic engineering vaccine for preventing staphylococcus caprae mastitis application

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Embodiment Construction

[0054] During the research and development of this vaccine, the selected genes were based on the following fact: ClfA, Hla and Sbi are virulence factors produced by Staphylococcus aureus, and play a virulence role with specific domains in the process of Staphylococcus aureus infecting the host. The A region of ClfA (ClfA-A) is the functional region that binds to provangelin and fibrinogen in the host extracellular matrix; Hla forms a membrane-attacking complex through the interaction between each monomer, and punches holes on the surface of the target cell. Its cleavage; the I and II regions of Sbi specifically bind to the Fc region of the host's IgG, interfering with the antibody-mediated opsonophagocytosis so as to resist the killing effect of the host phagocytes.

[0055] 1. Design primers

[0056] According to the already submitted on Genebank ClfA , Hla and Sbi Gene sequence design primers H1 / H2, CA1 / CA2 and S1 / S2 respectively, and add corresponding enzyme cutting si...

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Abstract

The invention belongs to the technical field of animal genetic engineering, and particularly relates to a preparation method of a genetic engineering vaccine for preventing staphylococcus caprae mastitis application. The preparation method comprises the following steps: 1) amplifying target genes ClfA, ClfA and Sbi; 2) building a Pci-Hla/ClfA-A/Sbi-I-II recombinant eukaryotic expression vector; and 3) establishing an immune procedure, and evaluating the immune result. According to the preparation method provided by the invention, the genetic engineering vaccine is on the basis of rapidly developed molecular biological technique, hereditary and immunology, and comprises a genetic subunit vaccine, a genetic mutation vaccine and a nucleic acid vaccine, wherein the nucleic acid vaccine is a DNA (Deoxyribose Nucleic Acid) vaccine, is an eukaryotic expression vector for to recombining and building one or more antigen-encoding genes, and can be directly injected into an organism to activate an immune system, so as to achieve the purpose of preventing and treating disease.

Description

1. Technical field: [0001] The invention belongs to the technical field of animal genetic engineering, and in particular relates to a preparation method and application of a genetic engineering vaccine for preventing goat staphylococcus mastitis. 2. Background technology: [0002] Mastitis is one of the animal diseases that cause headaches for dairy animal farmers, causing the milk production of female animals and the corresponding quality of dairy products to decline, resulting in significant economic losses. Staphylococcus aureus, Escherichia coli, and streptococcus are the main pathogenic bacteria that cause breast infection and cause clinical and subclinical mastitis. Because Staphylococcus aureus is resistant to the inhibitory effects of multiple antibiotics, non-antibiotic approaches to control mastitis caused by Staphylococcus aureus infection have become very important. [0003] Traditional mastitis vaccines are attenuated live vaccines or inactivated vaccines of th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K48/00A61K39/085A61P31/04
Inventor 陈德坤许君艳姚运亮田婷婷李前瑞罗军曹斌云
Owner NORTHWEST A & F UNIV
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