89 results about "Bio transformation" patented technology

The invention relates to processes that efficiently convert carbon-containing materials, such as biomass, into products in such a manner that the energy, carbon, and mass content of the materials are efficiently transferred into such products. Such methods include converting the materials into at least one intermediate by a biological conversion process and at least one intermediate by a thermochemical conversion process and reacting the intermediates to form the product. Such methods have a chemical energy efficiency to produce the product that is greater than the chemical energy efficiency of a solely biological conversion process to produce the product and that is greater than the chemical energy efficiency of a process in which all of the material is initially subjected to a thermochemical conversion step as part of the process to produce the product.

The invention discloses a gene segment provided with coded and highly-active trans-4-hydroxyl-L-prolyl hydroxylase and an application thereof. Gene codons of trans-4-hydroxyl-L-prolyl hydroxylase (GenBank ID: BAA 20094.1) in Dactylosporangiumsp. RH1 are optimized to obtain a p4hyd gene; the optimized p4hyd gene is truncated to obtain a P4Hyd1-257 gene; recombinant plasmids including pET-M-3C-P4Hyd and pET-M-3C-P4Hyd1-257 are built respectively, prokaryotic expression is performed through escherichia coli, and trans-4-hydroxyl-L-proline is produced in a conversion manner by utilizing thalli as an enzyme source. An experimental result shows that L-proline is taken as a substrate, compared with pET-M-3C-P4Hyd, the highest conversion rate of trans-4-hydroxyl-L-proline generated by pET-M-3C-P4Hyd1-257 in the conversion manner is increased from 91.4% to 97.4%, the conversion time is shortened from 80 hours to 60 hours, so that the conversion rate is improved remarkably, and the conversion time is shortened remarkably. The gene segment provided with coded and highly-active trans-4-hydroxyl-L-prolyl hydroxylase and the application thereof can be used for producing L-proline by a bioconversion method, and have better industrial application prospect.

The invention pertains to the field of biological transformation technology, which relates to a preparation method and the usage of fermentation liquid and fermentation liquid extract of ginkgo biloba leaf transformed by hericium erinaceus. The invention inoculates the hericium erinaceus in a wheat bran solid culture medium to be cultured respectively for 3 to 7 days, after that, the mixture is firstly trans-inoculated in a seed culture medium to be cultured for 1 to 2 days and is finally trans-inoculated in a fermentation culture medium to be cultured, wherein, the seed culture medium composition is: 10 to 40g / L of glucose, 1 to 5g / L of wheat bran, 2 to 10g / L of peptone and 5 to 20g / L of corn flour; the fermentation culture medium is: 10 to 40g / L of glucose, 1 to 5g / L of wheat bran, 2 to 10g / L of peptone, 5 to 20g / L of corn flour, 0 to 1g / L of MgSO4.7H2O, 0.1 to 2g / L of KH2PO4, 1 to 10g / L of CaCO3 and 0.2 to 5 percent EGB. The method of the invention has low cost, simple process, convenient management and can save manpower and material resources; furthermore, the tests for rats with diabetes prove that the prepared fermentation liquid can be used in the preparation of the drugs for lowering blood glucose.

The invention relates to processes that efficiently convert carbon-containing materials, such as biomass, into products in such a manner that the energy, carbon, and mass content of the materials are efficiently transferred into such products. Such methods include converting the materials into at least one intermediate by a biological conversion process and at least one intermediate by a thermochemical conversion process and reacting the intermediates to form the product. Such methods have a chemical energy efficiency to produce the product that is greater than the chemical energy efficiency of a solely biological conversion process to produce the product and that is greater than the chemical energy efficiency of a process in which all of the material is initially subjected to a thermochemical conversion step as part of the process to produce the product.

The invention relates to a method for improving the transformation efficiency of (S)-phenyl glycol by coupling glucose-6-phosphate dehydrogenase and (S)-carbonyl reductase, belonging to the technical field of biological catalytic asymmetric transformation. The invention provides the method for preparing the (S)-phenyl glycol by recombinant Pichia pastoris with CGMCCNo.4198 and 2-hydroxyacetophenone catalyzed by the recombinant Pichia pastoris with CGMCCNo.4198 in multiple batches. In the invention, the Saccharomyces cerevisiae glucose-6-phosphate dehydrogenase gene and the Candida parapsilosis (S)-carbonyl reductase gene are simultaneously integrated into the Pichia pastoris genome, under the participation of 5% (w / v) glucose, the whole cells are reused for 10 batches, and the optical purity and yield of the (S)-phenyl glycol prepared by asymmetric transformation are maintained at 100% and higher than 85% all the time. The recombinant strain improves the batch stability of full cell transformation of the (S)-phenyl glycol by polygene coexpression, which well relieves the limit of coenzyme regenerative cycle in a biological transformation reaction; and simultaneously an efficient approach for preparing the (S)-phenyl glycol industrially in multiple batches at low cost is provided.

The invention belongs to the technical field of bio-pharmaceuticals, and in particular relates to a method for producing a dexamethasone epoxide hydrolysate through one-step microbial fermentation and transformation. According to the method, with 16a-methyl-17[alpha],21-dihydroxypregna-4-ene-9,11-epoxy-3,20-dione-21 acetate as a substrate, the dexamethasone epoxide hydrolysate is directly produced mainly by conducting mixed fermentation by virtue of arthrobacter simplex and bacillus megatherium. In comparison with the prior art, the method provided by the invention, on the basis of a synergistic effect between mixed strains, integrally combines two steps of reactions, namely dehydrogenation and hydrolysis, so that the finished dexamethasone epoxide hydrolysate is directly obtained; and the product has the advantages of being short in production cycle, high in bio-transformation rate, high in yield, environment-friendly and low in cost.

The invention relates to a method for preparing natural 4-vinyl guaiacol by biological transformation. According to the method disclosed by the invention, Bacillussp. HY3CCTCCM2012247 is taken as a biocatalyst, natural ferulic acid is taken as a substrate, and the natural 4-vinyl guaiacol is prepared by microbial transformation. The production process comprises the following steps of: (1) performing slant culture; (2) preparing a seed culture solution; (3) culturing transformation cells; and (4) transforming. The method disclosed by the invention belongs to the technical field of biology, thenatural 4-vinyl guaiacol can be produced with high transformation rate by adopting the method, and the method can be used in the fields of tobacco, wine, foods and the like and further has great application prospects.

The invention discloses a corynebacterium glutamicum engineering bacterium for anaerobic conversion to produce succinic acid, and a building method and application thereof, and belongs to the field of genetic engineering. A pyruvate carboxylase gene of the corynebacterium glutamicum and phosphoenolpyruvate carboxylase from escherichia coli are cloned to corynebacterium glutamicum (ATCC13032); a lactate dehydrogenase gene of the corynebacterium glutamicum is knocked out in a homologous recombination manner. By adopting the lactic dehydrogenase-defective corynebacterium glutamicum for coexpression of a carboxylase gene, anaerobic production of succinic acid is carried out in a cell reutilization manner, so that the yield of succinic acid can be greatly improved; the yield can be up to 75g / L; the conversion rate of saccharic acid is 75%; the corynebacterium glutamicum engineering bacterium has a good application prospect; a fermentation model, especially a fermentation model for cell reutilization is built according to the optimum condition for biological transformation of succinic acid; the acid-production performance in repeated batch transformation process of cells can be basically kept stable.

The present invention relates to a biocatalyst displayed on spore or virus surface and a method of bioconversion using the same, in particular to a method of bioconversion using a biocatalyst, which comprises the steps of: (a) preparing a vector for spore surface display comprising a gene construct containing a gene encoding a display motif and a gene encoding the biocatalyst, wherein, when expressed, the gene construct expresses the display motif and the biocatalyst in a fusion form and the biocatalyst is displayed on a spore surface; (b) transforming a host cell with the vector for spore surface display; (c) displaying the biocatalyst on the spore surface of the host cell; (d) recovering the spore displaying on its surface the biocatalyst; and (e) performing the bioconversion reaction using the spore displaying on its surface the biocatalyst, and a biocatalyst.

A flow wood processing extracting lignin from woody plant material and converting delignified cellulosic residue to crude bio-oils, that removes toxic preservative chemicals from waste timber, and provides conversion to useful or nontoxic forms. Wood is chipped and fed into a lignin extractor using ethanol at high temperatures to dissolve the lignin with counter current material contactors, heat exchangers, and a computer control system. Most of preservative chemicals likely precipitate out at this stage as a heavy sludge that can be removed. Ethanol containing dissolved lignin is removed from the lignin extractor, the dissolved lignin recovered, the ethanol recycled into the lignin extractor, and residual heat returned to the process. Delignified cellulosic pulp is removed from the lignin extractor and subjected to a milling to convert into a smooth sludge for entry to a bio-convertor by a super critical water process. The residue is prepared as a high phosphate fertilizer.

The present invention belongs to the technical field of perfume for tobacco, and particularly relates to endophyte bio-transformed raisin extract and preparation and application in flavoring thereof. The method is implemented as follows: (1) separating and purifying endophyte; (2) culturing endophyte; (3) extracting and refining raisin; and (4) bio-transforming raisin extract with the endophyte to obtain bio-transformed raisin extract. The method of the present invention has simple technological process and low cost, and the obtained bio-transformed raisin extract can be directly used for theflavoring and casing of cigarettes. The perfume of bio-transformed raisin extract has a unique fresh and sweet smell, and can lower stimulation after being added to cigarettes. The perfume of bio-transformed raisin extract has a strong fresh and sweet smell as well as a strong toasting smell.

The invention relates to biofermentation field, especially relates to the submerged fermentation craft of the drumstick quern which appended balsam pear, and the fermented product of the craft. The characteristic is: take drumstick mushroom as start strain, do liquid shaking culture, liquid shaking enlargement culture, stair seed culture and the ferment culture which is added with balsam pear. The invention solves the problem of the drumstick mushroom's cosmically fluid culture, greatly improves the fermentation liquor activity because the traditional medicine balsam pear in the fermentation culture medium, together with the drumstick mushroom have done biological transformation, it has important sense to the drumstick mushroom's industry exploitation. The fermentation liquor is tremendous or brown liquid, and the mycelium is tremendous. The dry weight of the fermentation liquor mycelium is 7 to 10 gram per liter, and the restrain rate to extraneous non-enzyme glycosylation of the fermentation liquor is up to 93 to 98 percent.

The invention relates to Lactobacillussp.W2. The preservation number of the Lactobacillussp.W2 is CCTCCM209318. The invention also relates to application of the Lactobacillussp.W2 in biologically transforming phenylpyruvic acid substrate and extracting phenyllactic acid from fermentation solution by using ethyl acetate. The Lactobacillussp.W2 is from the traditional fermented food, belongs to an acknowledged safe strain and can be applied to food; the strain grows well on an MRS culture medium and is easily cultured and stored; and the content of the phenyllactic acid obtained by fermentation can reach 1.038 grams per liter, and the phenyllactic acid has strong capability of inhibiting putrefactive bacteria in the food.

The invention relates to a glutamate decarboxylase (GAD) thermally-stable variant G56P gene. Site-specific mutagenesis occurs at the 56th site of the nucleotide sequence of the GAD thermally-stable variant G56P gene. According to researches, the thermal stability of GAD coded through the variant gene obtained after mutagenesis at the site is enhanced. Through the site-specific mutagenesis on the basis of wild GAD gene coding, the thermal stability of the corresponding variant enzyme at 60-70DEG C is improved. With the improvement of the thermal stability of the enzyme, higher operating temperature can be adopted during industrial production to improve reaction speed and increase substrate solubility, and the production of GABA (gamma-aminobutyric acid) through biological transformation by using the enzyme is facilitated.

The invention relates to a 1, 3-propanediol oxidoreductase isozyme mutated gene and application thereof. By utilizing error-prone PCR technique-mediated random mutation, the1, 3-propanediol oxidoreductase isozyme mutated gene is obtained by screening. The codon GAC of the No. 99 aspartic acid in the gene is mutated as the codon CAA of glutamine; the codon AAC of the No. 147 asparaginate is mutated as histidine CAC; a nucleotide sequence of the mutated gene is SEQ ID No.1, and is capable of being encoded as 1, 3-propanediol oxidoreductase mutated isozyme. The invention creates 1, 3-propanedioloxidoreductase mutated isozyme that has relatively high catalytic activity to 3-Hydroxypropionaldehyde and can catalyze the 3-hydroxypropionaldehyde to produce chemical raw material 1, 3-propanediol.Compared with the 1, 3-propanediol reducase isozyme, the activity of one of the tested cases is increased by 4 to 4.6 times, which provides broad application prospect for bio-transformed production chemical material 1, 3-propanediol.

Improvements in biological conversion processes and associated apparatuses are disclosed for the generation of useful end products such as ethanol, through metabolic pathways of C1-fixing bacteria that utilize, as a nutrient, a C1-carbon source from a C1-containing substrate such as an industrial waste gas. Particular aspects of the disclosure relate to the downstream recovery of ethanol and / or isopropanol from bleed and permeate streams and more particularly to performing such recovery with improved efficiency that can advantageously reduce capital (e.g., equipment) and / or operating (e.g., utility) costs.

The invention discloses a fermentation method for a natural perfume gamma-decalactone, Namely, under the conditions that ((i)saccharomyces cerevisiae ( / i)) CGMCCNo.2.630 is used as a strain, and castor oil is used as a substrate, porcine pancreatic lipase is added, a natural perfume gamma-decalactone is prepared through a batch fermentation or fed-batch fermentation method in an aerobic environment. The fermentation method for a natural perfume gamma-decalactone disclosed by the invention reduces the cycle of transforming castor oil into natural gamma-decalactone and improves the ratio of transforming castor oil into natural gamma-decalactone and the yield of final products gamma-decalactone, wherein the biological transformation cycle of castor oil is 12-30h, the transformation rate of castor oil can reach 95%, and the biosynthesis efficiency of gamma-decalactone can reach 94.9%, especially in a 5L fermentation tank, the final yield of gamma-decalactone prepared through batch fermentation can reach 4.81g / L, and the final yield of gamma-decalactone prepared through fed-batch fermentation can reach 6.50g / L.

The invention discloses a biological transformation and synthesis method of rubescensin. The synthesis method comprises the following steps of: firstly, preparing a solid culture medium and a liquid culture medium; then, sterilizing and then inoculating the leaves, leaf buds or rhizome shucks of rabdosia rubescens into the prepared solid culture medium for culturing; transferring the callus obtained by culturing into the liquid culture medium for culturing; extracting the callus cultured in the liquid culture medium by adopting an organic solvent; and removing the organic solvent after extracting to obtain a rubescensin product (kaurene type diterpenoid compound). In the invention, the technical scheme for culturing and preparing rubescensin is free from the restriction of natural resources and is favorable for protecting the ecological environment. The technical scheme disclosed by the invention is easy to realize industrialized production, has the advantages of shorter culture time and higher product yield, lowers the production cost, and has obvious economic and social benefits.

The invention belongs to the application field of biology engineering technology, and particularly relates to a method of immobilizating gibberella CA3-1(Gibberella intermedia) with a preserved number of CGMCC No.4903 and using the gibberella for biological transformation to prepare nicotinic acid. The method of immobilizing the gibberella and using the gibberella for the biological transformation to prepare the nicotinic acid includes: immobilizing the gibberella CA3-1, adding a proper amount of 3-cyanopyridine, and choosing an optimum reaction condition for immobilizing thalluses to obtain a product which is the nicotinic acid. The method of immobilizing the gibberella and using the gibberella for the biological transformation to prepare the nicotinic acid strengthens tolerance of the thalluses to substrates after the thalluses are immobilized, improves production capacity of unit thalluses, simplifies separation processes of products greatly, increases reutilization times obviously, and provides a foundation for achieving technology of performing a biological transformation method by using the gibberella to prepare the nicotinic acid.

Improvements in biological conversion processes and associated apparatuses are disclosed for the generation of useful end products such as ethanol, through metabolic pathways of C1-fixing bacteria that utilize, as a nutrient, a C1-carbon source from a C1-containing substrate such as an industrial waste gas. Particular aspects of the disclosure relate to the downstream recovery of ethanol and / or isopropanol from bleed and permeate streams and more particularly to performing such recovery with improved efficiency that can advantageously reduce capital (e.g., equipment) and / or operating (e.g., utility) costs. Particular aspects related to the downstream recovery of ethanol and / or isopropanol using a low pressure separator having an internal divider.

Processes, as well as associated systems and computer program (software) products, are disclosed for the biological conversion of CO into desired end products such as ethanol. The control methodologies used for these processes can advantageously result in a reduced time required for a batch operation or other initial operating period, prior to achieving a continuous operation, which may be demarcated either by the addition of fresh culture medium at a defined flow rate or by another process initiation target. The control methodologies may alternatively, or in combination, improve a process performance parameter, such as productivity of the desired end product or bacterial growth rate, during this batch operation or other initial operating period.

The invention discloses a genetically engineered bacterium for producing protocatechuic acid (3, 4-dihydroxybenzoic acid) by using phenol as raw material and a construction method thereof. The engineering strain contains p-hydroxybenzoic acid decarboxylase gene yclBCD and hydroxylase gene pobA, phenol is used as raw material, phenol is carboxylated to obtain p-hydroxybenzoic acid by biological transformation, and then 3-hydroxybenzoic acid is oxidized to obtain protocatechuic acid (3, 4-dihydroxybenzoic acid). Compared with the traditional methods such as plant extraction and chemical synthesis, this method is convenient and quick, and will not pollute the environment, and can reasonably utilize phenol to save energy and protect the environment. It is of great significance to the production of protocatechuic acid.

The invention discloses a method for extracting ephedrine, which adopts a semi-bionic-biological enzyme extraction method, which overcomes the shortcomings of the semi-bionic method such as high extraction temperature under normal pressure and difficulty in separation and purification. The addition of enzymes is beneficial to the leaching and bio-enzyme extraction of active substances. Transformation can more effectively extract the ephedrine in ephedra without destroying the original ingredients.

The invention provides a polygonatum canaliculatum plant extracting solution, fermented yoghourt and a preparation method thereof, and relates to the technical field of microbial fermentation and biological transformation. The polygonatum canaliculatum plant extracting solution is mainly prepared from polygonatum canaliculatum by a heating water extraction method, and the fermented yoghourt prepared from the polygonatum canaliculatum plant extracting solution, fresh milk, sucrose and a compound fermentation strain has the effects of nourishing yin, reinforcing the spleen, moistening the lung and promoting the production of the body fluid, and has good health care effects on symptoms such as deficiency of body after disease, inappetence and hypoimmunity. The fermented product reserves the nutrient components of the original yoghourt, and simultaneously has partial effects of polygonatum canaliculatum, thereby well combining the health care effects of yoghourt and polygonatum canaliculatum. Due to good color, good mouthfeel and good flavor of the milk curd, the fermented product alleviates the problem that most of the existing health products have poor flavoring effects and poor acceptability of consumers.

The invention relates to a glutamate decarboxylase (GAD) thermally-stable variant G311P gene. Site-specific mutagenesis occurs at the 311th site of the nucleotide sequence of the GAD thermally-stable variant G311P gene. According to researches, the thermal stability of GAD coded through the variant gene obtained after mutagenesis at the site is enhanced. Through the site-specific mutagenesis on the basis of wild GAD gene coding, the thermal stability of the corresponding variant enzyme at 60-70DEG C is improved. With the improvement of the thermal stability of the enzyme, higher operating temperature can be adopted during industrial production to improve reaction speed and increase substrate solubility, and the production of GABA (gamma-aminobutyric acid) through biological transformation by using the enzyme is facilitated.

The invention belongs to the field of biological fermentation and in particular relates to scutellaria baicalensis and lactic acid bacteria yeasts, cosmetics containing yeasts, a preparation method and application. According to the technical problem to be solved, the biological transformation theory is taken as the guidance, a product with the effects of whitening, removing acnes and controlling oil is provided by adopting a liquid fermentation system of scutellaria baicalensis and lactic acid bacteria. The scutellaria baicalensis and lactic acid bacteria yeast refers to a fermented liquid mixture obtained by the step of fermenting a culture medium consisting of scutellaria baicalensis and water with lactic acid bacteria, wherein the culture medium comprises 5-10 weight percent of scutellaria baicalensis based on the weight of a crude drug, and the inoculation amount of the lactic acid bacteria is 1.0*10<4> to 1.0*10<8>cfu / ml.

The invention belongs to a preparation method for platycodin D, and adopts a mixed bacterial strain which includes a Pichia kudriavzevii bacterial strain and a Bacillus velezensis bacterial strain toprepare the platycodin D. According to the preparation method, two types of bacterial strains with different conversion mechanisms are used for the first time to convert platycodin in a mixed fermentation way, and the yield of the platycodin D and deapioplatycodin D can be effectively improved. By use of bacterial strain fermentation characteristics, the platycodin can be subjected to biological transformation, the safe and environmentally-friendly production of the platycodin can be realized, platycodon grandiflorum resources can be fully utilized, and the platycodin which is contained in theplatycodon grandiflorum resources and has low biological activity can be converted into saponin with high biological activity so as to achieve an effect of recycling waste materials.

The invention which belongs to the technical field of biological engineering relates to a process for o-cresol compound conversion with a biological method. The process is characterized in that: a first step biological conversion reaction is carried out to obtain a product 3-methycatechol with conditions that a dry weight of whole cells of phenol hydroxylase gene engineering bacteria PH-IND in a reaction solution is from 20 to 40 g / L, a concentration of o-cresol is from 50 to 700 mg / L, the concentration of glucose is from 5 to 40 mmol / L, a temperature is from 20 to 40 DEG C, a shaker speed iscontrolled from 50 to 200 r / min, and an oscillation reaction period is from 10 to 240 min; and a second step biological conversion reaction is carried out with the conditions that the dry weight of the whole cells of biphenyl dioxygenase gene engineering bacteria BphC-LA-4 which are added in the reaction solution is from 20 to 40 g / L, the shaker speed is controlled from 50 to 200 r / min, and the oscillation reaction period is from 5 to 50 min. The method has the advantages of mild reaction condition, single product and simple extraction step, allows the product to be recycled, and is suitable for industrial production and application.

The invention provides a tremella fuciformis biotransformation composition of maca and Chinese yam and a preparation method of the composition. According to the invention, a unique combination of macapowder, Chinese yam powder and soybean powder is employed for the first time, and a deep fermentation and bio-transformation technology using tremella fuciformis is utilized. Through reasonable matching of tremella fuciformis, maca and Chinese yam, and technologies of adjusting of the acidity and alkalinity step by step and temperature-raising heat-preservation conversion, the tremella fuciformisbiotransformation composition of maca and Chinese yam is manufactured, and the composition has various health-care functions. The invention not only provides a bioconversion extraction method for bioactive substances in Maca and Chinese yam, but also provides a method for improving tremella fuciformis polysaccharides. Animal experiments prove that the tremella fuciformis biotransformation composition of maca and Chinese yam is produced by the method disclosed by the present invention has functions of improving lung fibrosis function, resisting fatigue and the like.

Apparatuses and associated methods are described for the efficient evaluation of C1-containing substrates, and especially for such evaluation conducted locally, or on-site, at a prospective facility for implementation of a biological conversion process for desired end product using a C1 carbon source. The exact composition of a given, industrial C1-containing substrate, as well as the range in composition fluctuations, are generally difficult to reproduce at a remote facility (e.g., a laboratory or a pilot-scale or demonstration-scale process), as required for the accurate prediction / modeling of commercial performance to justify large capital expenditures for commercial scale-up.