91 results about "Pancrelipase" patented technology

The invention provides compositions and methods for treating pancreatic enzyme insufficiency, such as the pancreatic enzyme insufficiency associated with cystic fibrosis. The invention also provides compositions comprising lipase from Candida cylindracea, alone or in combination with amylase or amyloglucosidase, protease and / or lactase. Furthermore, the invention discloses methods for treating pancreatic enzyme insufficiency comprising administering compositions to patients in need thereof.

Compositions of the present invention, comprising at least one digestive enzyme (e.g., pancrelipase) are useful for treating or preventing disorders associated with digestive enzyme deficiencies. The compositions of the present invention can comprise a plurality of coated particles, each of which is comprised of a core coated with an enteric coating comprising at least one enteric polymer and 4-10% of at least one alkalinizing agent, or have moisture contents of about 9% or less or 3% or less, water activities of about 0.6 or less, or exhibit a loss of activity of no more than about 25%, about 20%, about 15% or about 10% after six months of accelerated stability testing and the titer level of a viral contaminant present in the pancreatin is at least about 1000 times less than the titer level of the viral contaminant present in a preparation from which the pancreatin is obtained.

Compositions of the present invention, comprising at least one digestive enzyme (e.g., pancrelipase) are useful for treating or preventing disorders associated with digestive enzyme deficiencies. The compositions of the present invention can comprise a plurality of coated particles, each of which is comprised of a core coated with an enteric coating comprising at least one enteric polymer and 4-10% of at least one alkalinizing agent, or have moisture contents of about 3% or less, water activities of about 0.6 or less, or exhibit a loss of activity of no more than about 15% after six months of accelerated stability testing.

The present invention provides methods for detecting viral infectivity and content in an enzyme preparation. In certain embodiments, the invention relates to methods for producing a pharmaceutical pancreatic enzyme composition. In additional embodiments, the invention relates to detecting infectious porcine parvovirus (PPV) and determining PPV content in pancreatic enzyme preparations (PEPs), including pancrelipase preparations.

A method for treating steatorrhea in infants in need thereof by administering to the infant an amount of pancrelipase of from about 300 to about 2,500 USP units lipase / kg / meal or from about 1,500 to about 7,500 USP units lipase / kg / day.

The invention relates to a method using pancreatin-hydrolyzed swine blood corpuscle protein activated by swine fresh pancreas to produce swine blood protein peptide and haemoglobin, belonging to the field of feed additive. The invention takes fresh healthy swine blood corpuscle liquid as a raw material, pancreatin activated by swine fresh pancreas is hydrolyzed and filtered, the filtered supernatant is decolorized, then, the obtained protein liquid is dried to obtain the golden swine blood protein peptide, and the filter cake is dried to obtain the haemoglobin. The technical method has the following innovation points: 1, swine pancreas is taken as the raw material which is fresh and available, and the quality is controllable; 2, pancreatin activation method is simple, enzyme systems are rich, the activated enzyme has high activity, and the hydrolysis effect is good; 3, the swine fresh pancreatin has low cost, which is easy to carry out expanded production; 4, the swine blood corpuscle protein is hydrolyzed by pancreatin which can efficiently separate the haemoglobin thereof and can further prepare haemoglobin by drying the filter cake as the hydrolysate product, thus greatly improving the rate of multipurpose utilization of the swine blood corpuscle protein and avoiding resource waste.

The invention provides microcapsule fatty powder capable of increasing content of omega3-enriched meat egg milk and a preparation method of the microcapsule fatty powder. The microcapsule fatty powder capable of increasing the content of the omega3-enriched meat egg milk comprises a water soluble wall material and an omega3-containing fat core material embedded in the wall material, wherein the water soluble wall material is at least one of dextrin, water soluble syrup, whey powder and chitosan, and then is mixed with starch paste, and thus the microcapsule fatty powder is obtained; the omega3-containing fat core material is at least one of linseed oil, deep sea fish oil and algal oil and is prepared by mixing a compound emulsifier with sodium stearoyl lactate. The microcapsule fatty powder capable of increasing the content of the omega3-enriched meat egg milk can be combined at small intestines and pancrelipase easily, the digestion absorption utilization rate of fat containing omega3 is greatly increased, and the fatty powder capable of increasing the content of the omega3-enriched meat egg milk is resistant to oxidation and easy to store and can not be oxidized or become acid, and thus the content of omega3 is guaranteed.

The invention provides a preparation method of ginsenoside, which is suitable for industrialized production. The ginsenoside is used as a main component to prepare weight-loss health foods or pharmaceutical compositions. Simultaneously, the ginsenoside has functions of restraining pancreatic lipase activity and reducing body fat.

The invention belongs to the technical field of medicine, and relates to a prenylflavonoid compound separated from artocarpus hypargyreus hance and having a structure of a formula (I) and application thereof. Pancrelipase inhibiting activity experiment results show that the compound has obvious pancrelipase inhibiting activity. The compound can be used as a pilot compound for preparing new medicaments for preventing or treating obesity and related metabolic diseases, and can also be used for preparing medicaments for preventing or treating clinically common obesity and related metabolic diseases.

The invention belongs to the technical field of medicaments, and in particular relates to application of isopentene group flavonoid compound used as a pancreatic lipase inhibitor. The flavonoid compound has the following structural formula and has a chemical name of: (E)-2-(2,4-dihydroxy phenyl)-5,7-dihydroxy-3-(3-methyl-2-butenyl)-6-(3-methy-l-butenyl)-4H-chromene-4-ketone, and can be extracted from artocarpus nitidus. Pancreatic lipase activity tests and experiments of weight loss action in animals prove that the compound has obvious pancreatic lipase inhibitory activity and weight loss action, can be taken as the lead compound for developing novel medicaments for preventing or curing obesity and related metabolic diseases, and also can be used for preparing the medicaments for preventing and curing clinically common multiple obesity and the related metabolic disease.

The invention provides a peptide with a pancreatic lipase inhibitor effect. The sequence of the peptide is selected from SEQ ID NO.1-5; the invention also provides application of the peptide in preparation of medicines, foods and health-care products for treating hyperlipemia. The peptide is a plant-derived, non-toxic and harmless natural substance, and experiments prove that the peptide has remarkable pancreatic lipase inhibitory activity and lipid-lowering effect; the compound is clear in action mechanism and definite in target spot, is expected to become a substitute medicine for preventing and treating hyperlipemia, and has a good market prospect in the industries of medicine and functional food.

The invention discloses a selective method for selecting the pancrelipase inhibitor from Chinese traditional medicine. The method is to enclose the pancrelipase on the Millipore enzyme standard board; by adding the activator, the pancrelipase can be transferred into the active open structure; after washing, the Chinese traditional medicine extraction is added to culture to remove the uncombined or weakly combined things; after washing, the substrate is added to react and detect the fluorescence intensity of product and compute the inhibiting percent of sample. The method can be used to select the Chinese traditional medicine extraction and decrease the false masculine result made by the surfactant and nonspecific thing in sample to improve the selective hit rate.

The invention relates to a laurane type sesquiterpene compound or a derivative thereof extracted and separated from a sea plant Laurencia okamurai in the East China Sea, and a preparation method and application thereof. The structural formula of the laurane type sesquiterpene compound or the derivative thereof is shown as above: when R1, R2 and R3 are hydrogen at the same time, the sesquiterpene compound or the derivative thereof is laurinferol, and when R4 is hydrogen, the sesquiterpene compound or the derivative thereof is aplysin. In vitro tests show that the sesquiterpene compound or the derivative thereof has obvious pancreatic lipase inhibition activity and is expected to be applied to preparing medicaments for preventing or treating decompensation diseases including adiposis, diabetes, hyperlipoidemia, fatty liver and the like. The sesquiterpene compound or the derivative thereof can also provide a lead compound for developing the new medicaments for preventing and treating the decompensation diseases, and is significant for developing and utilizing marine biological resources of China.

The invention discloses an application of porcine pancreatic lipase as a catalyst of asymmetric aldol reaction of heterocyclic ketone and aromatic aldehyde. The aromatic nucleus of the aromatic aldehyde is connected with an electron withdrawing substituent, and the aromatic aldehyde has the advantages of good catalytic activity, high enantiomeric selectivity, environmental friendliness, good economical performance and the like. The invention also provides a method of using the porcine pancreatic lipase to catalyze the asymmetric aldol reaction of the heterocyclic ketone and the aromatic aldehyde, the yield is high, and the ee (enantiomeric excess) value is high. The scope of application of the porcine pancreatic lipase is broadened, a potentially excellent catalyst is provided for the asymmetric aldol reaction, and the porcine pancreatic lipase has broad application prospects in the field of asymmetric synthesis.

The invention discloses an application of porcine pancreas lipase as a catalyst for a Michael addition reaction of 4-hydroxycoumarin and alpha, beta-nonsaturated ketone, the catalytic activity is good; the invention also provides a method for synthesizing an anticoagulation agent warfarin and its derivative by using porcine pancreas lipase for catalyzing the Michael addition reaction, the yield is high, the application scope of the porcine pancreas lipase is widened, a potential excellent catalyst is provided for the Michael addition reaction, and a method with the characteristics of high efficiency, environmental protection and economy is provided for the synthesis of the warfarin and its derivative, and possesses wide application prospect in the organic synthesis field.

The invention provides a method for preparing biodiesel from immobilized lipase catalyzed pistacia chinensis bunge. The method is characterized in that the biodiesel is prepared by transesterification reaction of immobilized lipase catalyzed pistacia chinensis bunge which takes natural fiber-ramie as a vector and methanol. According to the invention, immobilized lipase can be recovered after esterification reaction and then continuously and repeatedly used thirteen times, and the activity maintaining rate of lipase is larger than 80.0%, thereby improving the utilization of lipase and reducingthe product cost resulting from lipase.

The invention discloses a fermentation method for a natural perfume gamma-decalactone, Namely, under the conditions that ((i)saccharomyces cerevisiae ( / i)) CGMCCNo.2.630 is used as a strain, and castor oil is used as a substrate, porcine pancreatic lipase is added, a natural perfume gamma-decalactone is prepared through a batch fermentation or fed-batch fermentation method in an aerobic environment. The fermentation method for a natural perfume gamma-decalactone disclosed by the invention reduces the cycle of transforming castor oil into natural gamma-decalactone and improves the ratio of transforming castor oil into natural gamma-decalactone and the yield of final products gamma-decalactone, wherein the biological transformation cycle of castor oil is 12-30h, the transformation rate of castor oil can reach 95%, and the biosynthesis efficiency of gamma-decalactone can reach 94.9%, especially in a 5L fermentation tank, the final yield of gamma-decalactone prepared through batch fermentation can reach 4.81g / L, and the final yield of gamma-decalactone prepared through fed-batch fermentation can reach 6.50g / L.

The invention relates to a method for resolving DL-menthol through the catalysis of lipase, and belongs to the technical field of biological catalysis, solving the technical problems of very difficult acylating agent separation and cumbersome step of the traditional method for resolving the DL-menthol. The method comprises the following steps of 1, mixing the LD-menthol, porcine pancreatic lipase and succinic anhydride with ether, then shaking at the rotation speed of a shaking table, carrying out centrifugal filtration to remove the porcine pancreatic lipase, diluting by using diethyl ether, adding an NaCO3 solution, then extracting for three times by using the diethyl ether, then drying by using MgSO4, and then carrying out decompressing rotary evaporation to remove the diethyl ether to obtain D-menthol; 2, adding an NaOH solution to a water phase for stirring and hydrolysis, then extracting for three times by using the diethyl ether, drying by using MgSO4, and carrying out the decompressing rotary evaporation to remove the diethyl ether to obtain L-menthol. The method disclosed by the invention is used for obtaining the D-menthol and the L-menthol.

The invention discloses effect of long-chain inulin in adjusting acute pancreatitis and related tissue damage caused thereby, and belongs to the technical field of prevention and treatment of acute pancreatitis. It is verified herein that long-chain inulin can protect from caerulein-induced acute pancreatitis in mice and may be added, as an effective ingredient, to drugs for preventing and treating acute pancreatitis, thereby reducing occurrence and progress and degree of clinical acute pancreatitis. The long-chain inulin is used to carry out preventive treatment for a caerulein-induced mice acute pancreatitis model, pathomorphologic observation is carried out on pancreatic tissues of mice, the levels of serum pancrelipase, serum amylase and tissue inflammatory cell liberase in mice are measured, anti-inflammatory cytokine IL10 and pro-inflammatory cytokine TNF-Alpha and IL1 Beta in tissues and serum are also measured. By comparing index levels of mice in an insulin treatment group and an inflammatory model group, the indexes of mice in the inulin treatment group are significantly better those of the inflammatory model group.

The subject invention features compounds having the structure: wherein X is O, S, CH2 or NR5; Y is O or S; R1 is H, substituted or unsubstituted C1-C15 alkyl, C1-C8 alkylaryl, —C(O)OR4, —C(O)NR4R5, —CR6R6′OR4, —CR6R6′OC(O)R4, —CR6R6′OC(O)NHR7, —C(O)NR10R11, —C(O)NR8R9NR8R9, —N(R5)C(O)NHR5, or CH2R4; R2 is a substituted or unsubstituted, straight chain C1—C30 alkyl or branched C3-C30 alkyl, aryl, alkylaryl, arylalkyl, heteroarylalkyl or cycloalkyl; R3 is H or substituted or unsubstituted C1-C6 alkyl or C3-C10 cycloalkyl; R4 is H or a substituted or unsubstituted, straight chain or branched, C6-C30 alkyl, aryl, —CH2-aryl, aryl —C1-C15 alkyl, heteroaryl-C1-C15alkyl or C3-C10 cycloalkyl; R5 is H or a substituted or unsubstituted, straight chain or branched, C6-C30 alkyl, aryl C1-C30alkyl, heteroarylalkyl or cycloalkyl; R6 and R6′ are each independently H, substituted or unsubstituted C1-C6 alkyl, dialkyl or C3-C10 cycloalkyl or together form a 3-7 membered ring system; R7 is H or substituted or unsubstituted C1-C12 alkyl or C3-C10 cycloalkyl; R8 and R9 are each independently H, substituted or unsubstituted C1-C6 alkyl, C1-C6 alkoxy, C1-C6 alkylaryl, or NR8R9 together form a substituted piperazine or piperidine ring or a dihydro-1H-isoquinoline ring system, or a specific enantiomer thereof, or a specific tautomer, or a pharmaceutically acceptable salt thereof and a method for treating diabetes or obesity by administering a therapeutically effective amount of the compounds of the invention.

The invention provides meroganocochlearin which is separated from an ethyl acetate layer of Ganoderma cochlear and is novel in structure, a pharmaceutical composition which takes the meroganocochlearin as an active ingredient, a preparation method of the pharmaceutical composition and application thereof in pharmacy. Through in vitro experiment research, the meroganocochlearin can effectively inhibit activity of alpha-glucosidase and pancrelipase, and therefore can be used for treating diabetes and obesity and used as health-care functional food.

The invention provides application of a ginkgo biflavonoid compound in the preparation of slimming medicines and / or sliming medicine compositions. The ginkgo biflavonoid compound has the advantages that the activity of pancreatic lipase can be effectively inhibited, and the IC50 of inhibited pancreatic lipase is 2.90mum to 12.78mum; especially, the oral dose of the ginkgo biflavonoid is 4.5 to 12.5mg, the local exposure amount in the gastrointestinal system can reach 2.27 to 7.67mum, and the inhibiting constant is higher than the inhibiting constant (Ki=0.81mum) of the ginkgo biflavonoid on the pancreatic lipase, which indicates that the in-vitro medicine exposure concentration of the ginkgo biflavonoid can inhibit the pancreatic lipase at high efficiency, and further improve the digestionand absorption of lipid in the human body; the raw materials are easy to obtain, the safety is high, the extracting and preparation technologies are simple, the yield rate is high, and the like; a novel medicine source is provided for the preventing and controlling on the fat, and the good application prospect is realized in the preparation of the slimming medicines.

The invention discloses an anti-obesity peptide and application thereof, the amino acid sequences of five peptides are respectively RGGFLLAMVRAP, QSAFLHVMY, FLLWAAL, WLTAMLEWR and WYFPGR, and the peptide provided by the invention is synthesized by using a polypeptide synthesizer and adopting a solid-phase synthesis method. In-vitro activity detection shows that the 50% inhibition concentration of the five peptides to pancreatic lipase is within the range of 5.36-477.24 mu M. Under the concentration of 200 [mu] M, the content of triglyceride TG in 3T3-L1 cells can be reduced from 1.06 + / -0.06 mmol / gprot to 0.17 + / -0.02 to 0.69 + / -0.01 mmol / gprot by the five peptides. The invention provides five synthetic peptides with potential in-vitro anti-obesity activity, which can be applied to the field of biological pharmacy.

The invention discloses a kit for detecting canine pancreatic lipase based on a homogeneous chemiluminescence immunoassay method. The kit comprises a DNA1-cPL antibody 1 conjugate, a DNA2-cPL antibody2 conjugate, acridinium ester (AE) labeled DNA3 and a graphene oxide (GO) binding antioxidant (AOD). The kit disclosed by the invention is used for detection, and the kit has the advantages of simpleoperation, accurate result, short time consumption, high precision and the realization of quantitative detection.

The invention discloses a preserved meat product preservation method. The method comprises the following steps: (1) screening a culture for fermenting a preserved meat product, and establishing preserved meat fermentation conditions; (2) determining the types and the amount of enzymes and enzymolysis conditions, adding papain, pancrelipase, calpains, pronase and other enzyme preparations into the salting process by taking the texture, flavor, color and the like as indexes, and determining the enzyme addition amount, the enzymolysis time, the enzymolysis temperature and other enzymolysis method conditions; (3) analyzing the acid value and change of peroxide value in the preserved meat production process, and selecting an antioxidant; and (4) preparing an edible film, establishing a coating technology, wherein microbes, the acid value, the peroxide value and the TBA value are used as indexes, different composite edible film materials such as chitosan, modified starch and soy isolate protein are used, and nisin or nisin are added, and performing antiseptic validity treatment on the product by adopting dipping, spraying and other different coating technologies. The method is easy to operate and obvious in effect.

The invention relates to a new family of proteolytic enzymes having the ability to hydrolize at a p H between 3 and 8 gluten olygopeptides which are resistant to cleavage by gastric and pancreatic enzymes and whose presence in the intestinal lumen results in toxic effects. The enzymes have been identified as endopeptidases of the S8 / S53 family and are produced by an Actinoallomurus strain. The object of the invention includes also methods for producing enzymes composition comprising the endopeptidases by cultivation of native Actinoallomurus strains, mutants thereof, or recombinant host cells comprising nucleic acids codifying for the endopeptidases. Said nucleic acids constitute a further object of the invention. The enzyme compositions comprising at least one endopeptidase of the invention are useful for the treatment and / or prevention of celiac sprue, dermatitis herpetiformis and any other disorder associated with gluten intolerance as ingredients of pharmaceutical formulations or as additives of foods and drinks.

The invention relates to the technical field of biology, in particular to an application of lycium ruthenicum seed oil extracts in hypolipidemic medicines. The lycium ruthenicum seed oil extract fattyacid and sterol can be used as effective components to be prepared into hypolipidemic medicinal preparations with any pharmaceutically-acceptable carrier according to a conventional method. The lycium ruthenicum seed oil extract fatty acid and sterol can obviously inhibit the activity of pancrelipase and cholesterol esterase targets related to hyperlipidemia. Besides, zebrafish and mouse experiments prove that the lycium ruthenicum seed oil fatty acid and sterol have obvious blood fat-reducing effect; and the lycium ruthenicum seed oil fatty acid and sterol are used as active components for reducing blood fat, have good effect of reducing blood fat, have obvious effect, clear action mechanism and clear targets, and meet the requirement of development of pharmaceutical preparations.