83 results about "Phenylpyruvic acid" patented technology

The object of the present invention is to provide a novel dehydrogenase having a property which is different from that of known dehydrogenases. The present invention provides a dehydrogenase having the following physicochemical properties: (1) effect: to produce N-alkyl-L-alanine from pyruvic acid and alkylamine or dialkylamine using NADPH and / or NADH as coenzyme; (2) substrate specificity: to show activity to alkylamine or dialkylamine but not to ammonium; (3) optimal pH when using phenylpyruvic acid and methylamine as substrates is around 10; and (4) when treated at 30° C. for 30 minutes, the enzyme is stable at around pH 5 to 10.5.

Process for enhancing the flavour of a cheese or of a cheese-flavoured food product whose preparation comprises a maturation step in the presence of lactic acid bacteria, characterized in that a preparation additive comprising at least one keto acid chosen from the group consisting of alpha-ketoglutaric acid, alpha-ketoisocaproic acid ketoisovaleric acid and phenylpyruvic acid is used to increase the catabolism of the amino acids in the cheese or food product by the said bacteria.

Disclosed is a method for producing phenyllactic acid by controlling pH value and fed-batch fermentation, which belongs to the food biotechnology field. The method uses lactobacillus as a strain which is inoculated in a fermentation tank with the volume of liquid ranging from 1 to 10L, the agitation speed is between 50 and 150 rpm, 6 to 10mol / L of sodium hydroxide is added after 6 to 12h, and the pH value is controlled at between 5.5 and 6.5; and glucose and substrate phenylpyruvic acid are supplemented to the fermentation tank in batch at the period between 12 to 54h in the fermentation process, and a fermentation broth containing phenyllactic acid is obtained after the cultivation for 70 to 90h. The method has the advantages that: the high-concentration substrate inhibition is eliminated and nutritive materials are also supplemented through controlling the pH value in the fermentation environment and supplementing a carbon source and the substrate, so the thalli can grow in the suitable environment, the content of phenyllactic acid reaches 6 to 20g / L after the fermentation, the process is simple, the environmental pollution is less, the operation is convenient, and the output of phenyllactic acid is high.

The invention relates to a hydrocarbon oil demetalization agent and a method for hydrocarbon oil demetalization. The hydrocarbon oil demetalization agent is a keto acid, wherein the keto acid is any of alpha-keto acid, beta-keto acid and gamma-keto acid, and the alpha-keto acid is one of pyruvic acid, 2-ketobutyric acid, phenylpyruvic acid and benzoyl formic acid; the beta-keto acid is acetoacetic acid; and the gamma-keto acid is 4-ketovaleric acid. The method comprises the steps of carrying out mixed contact on hydrocarbon oil, water, the keto acid, which serves as the demetalization agent, and a demulsifier, and then, carrying out electric desalting, so as to achieve oil-water separation and remove metals from the hydrocarbon oil. A molecule of the demetalization agent disclosed by the invention contains a difunctional group, can be in chelation with the metals and can also be in complexation with the metals, so that the demetalization agent has a high removal ratio to the metals in the hydrocarbon oil; according to the demetalization agent, the acidity is between the acidity of organic acids and the acidity of inorganic acids, the corrosiveness is lower than that of the inorganic acids, and the demetalization rate is higher than that of the organic acids; and meanwhile, the demetalization agent is free of elements such as phosphorus, sulfur and nitrogen and thus cannot cause adverse effects on subsequent processing.

The invention relates to metabolism of enteric microorganisms and phenylalanine and phenylpyruvic acid of the enteric microorganisms, and provides a composition containing phenyllactic acid or salts of phenyllactic acid and phenylpyruvic acid or salts of phenylpyruvic acid and used for preventing or / and treating insulin resistance and related diseases, use of the composition in preparation of a medicine or food for improving, preventing or treating insulin resistance and related diseases, and a method for preventing or treating insulin resistance and related diseases. The related diseases include intestinal dysbacteriosis, metabolic syndrome, obesity, diabetes, podagra and tumors.

The invention discloses a microbial cell cryogel for conversion synthesis of phenyllactic acid, a preparation method and application thereof. The microbial cell cryogel is an integral porous material,the matrix skeleton is a cross-linked Lactobacillus casei microbial cell, the pore size ranges from 1 to 50 micrometers, the porosity is 36.9-95.2%, and the water permeability is 1*10<-15>-3*10<-13>m<2>. Serving as a biocatalyst, the cryogel can achieve conversion synthesis of a phenylpyruvic acid key substrate into phenyllactic acid, and the maximum conversion yield can reach 75%. The microbialcell integral cryogel provided by the invention has the advantages of large pore size, high porosity, fast substrate and product mass transfer, good bio-safety, reusability, simple preparation method, and low cost, and has broad application prospects in the biochemical synthesis field.

The present invention refers to method for producing a transgenic plant with increased herbicide tolerance or resistance as compared to a corresponding non-transformed wild type plant, comprising transforming a plant cell or a plant cell nucleus or a plant tissue with a nucleic acid molecule encoding a HPPD polypeptide, as well as to the nucleic acid, and plants with increased HPPD-inhibiting herbicide tolerance or resistance comprising the nucleic acid of the invention.