The invention discloses a method for improving conversion rate of
chiral amine. The method comprises the following steps: synthesizing and amplifying an optimized ArR-
omega TA
nucleotide sequence containing
enzyme cutting sites, constructing p2A4-ArR-
omega TA, p2A4-FA and p2A4-ldh, wherein FA is a
gene for expressing
formic acid acetyl
transferase, and correspondingly introducing
escherichia colito obtain recombinant
bacteria 1, FA and 6; fermenting to obtain
fermentation liquor of the recombinant
bacteria 1, FA and 6; centrifuging to obtain
fermentation liquor precipitates of the recombinantbacteria 1, FA and 6; and correspondingly preparing crude FA
enzyme liquid and crude ldh
enzyme liquid; resuspending the
fermentation liquor precipitate of the recombinant
bacteria 1 by using PBS, adding PLP and NAD< +>, and reacting; and adding the crude FA enzyme liquid, the crude ldh enzyme liquid,
formate dehydrogenase, D-
alanine, a substrate
ketone and CoA, adding a cosolvent, reacting, terminating, extracting and
drying. According to the method disclosed by the invention, a byproduct
pyruvic acid is removed, and the conversion rate of the
chiral amine is improved. The
formate dehydrogenase is added to recover a
cofactor reduced
coenzyme I.