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Prepn process of compound degrading enzyme

A compound enzyme and production method technology, applied in the direction of biochemical equipment and methods, enzymes, enzymes, etc., can solve the problems of negligible degradation of pesticides and secondary pollution, and achieve high activity units, stable product quality, and high degradation. Effect

Inactive Publication Date: 2005-04-27
陈玉柱
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The Ministry of Agriculture has formulated 8-10 years to basically realize the pollution-free action of major agricultural products. Now there are many pesticide degradation machines on the market: mainly ordinary detergents and special fruit and vegetable detergents. The main functions of these two types of products are decontamination and sterilization. However, the degradation effect on pesticides is negligible, because the ingredients of these products are chemical substances, not only cannot remove pesticide residues, but also cause secondary pollution if they are not thoroughly rinsed.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] The present invention adopts the following design scheme: select the following raw materials and their weight ratios as components of the inventive production method: lignin peroxidase 60 phytase 8 phenol oxidase 12 formamidase 60 cyanide hydrolase 60 formate dehydration Hydrogenase 60 Lysozyme 10 Tween solution 200 (concentration 50-1000mg / L).

[0016] Then prepare as follows:

[0017] 1. Material selection, select raw materials according to the requirements of the above components, the quality of each single enzyme must be of high quality, the active unit should be 50,000-150,000 units / g, the fineness should be 60-200 mesh, and the Tween concentration should be At 50-1000mg / L.

[0018] 2. Mixing and liquefaction The raw materials selected according to the important ratio are put into the closed low-temperature stirring liquefier for stirring and liquefaction. The solubility of the enzyme should be 96-98%.

[0019] 3. Cultivation: Pour the liquefied solution into the...

Embodiment 2

[0023] The present invention adopts the following design schemes: select the following raw materials and their weight ratios as components of the inventive production method: lignin peroxidase 60 phytase 8 phenol oxidase 12 formamidase 30 cyanide hydrolase 30 formate dehydration Hydrogenase 30 Lysozyme 8 Tween solution 100 (concentration 50-1000mg / L).

[0024] Then prepare as follows:

[0025] 1. Material selection, select raw materials according to the requirements of the above components, the quality of each single enzyme must be of high quality, the active unit should be 50,000-150,000 units / g, the fineness should be 60-200 mesh, and the Tween concentration should be At 50-1000mg / L.

[0026] 2. Mixing and liquefaction The raw materials selected according to the important ratio are put into the closed low-temperature stirring liquefier for stirring and liquefaction. The solubility of the enzyme should be 96-98%.

[0027] 3. Cultivation: Pour the liquefied solution into the...

Embodiment 3

[0031] The present invention adopts the following design scheme: select the following raw materials and their weight ratios as the components of the inventive production method: lignin peroxidase 50 phytase 7 phenol oxidase 9 formamidase 50 cyanide hydrolase 50 formate dehydration Hydrogenase 50 Lysozyme 9 Tween solution 150 (concentration 50-1000mg / L).

[0032] Then prepare as follows:

[0033] 1. Material selection, select raw materials according to the requirements of the above components, the quality of each single enzyme must be of high quality, the active unit should be 50,000-150,000 units / g, the fineness should be 60-200 mesh, and the Tween concentration should be At 50-1000mg / L.

[0034] 2. Mixing and liquefaction The raw materials selected according to the important ratio are put into the closed low-temperature stirring liquefier for stirring and liquefaction. The solubility of the enzyme should be 96-98%.

[0035] 3. Cultivation: Pour the liquefied solution into t...

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Abstract

The pesticide degrading compound enzyme is prepared with lignoperoxidase, phytase, phenoloxidase, formamidase, cyanide hydrolase, formic dehydrogenase, lysozyme, and Tween solution, and through mixing, culture, drying and packing. The product has high activity, high efficiency and wide degradation spectrum, and has the functions of killing bacteria, decontaminating, eliminating pollutant heavy metal, degrading pesticide residue, etc.

Description

[0001] Technical field The present invention designs a method for preparing a degrading compound enzyme. Background technique [0002] my country is a big country that uses pesticides, with an annual consumption of 800,000 to 1 million tons. Pesticide residues in fruit and vegetable foods exceed the standard by 47.5%, which poses a great threat to the physical and mental health of the Chinese people. The Chinese government has included the removal of pesticide residues in agricultural products as a " During the Tenth Five-Year Plan period, the national high-tech development plan was included in the <863> project. The Ministry of Agriculture has formulated 8-10 years to basically realize the pollution-free action of major agricultural products. Now there are many pesticide degradation machines on the market: mainly ordinary detergents and special fruit and vegetable detergents. The main functions of these two types of products are decontamination and sterilization. However...

Claims

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Application Information

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IPC IPC(8): A23N12/00A23N15/00C12N9/00
Inventor 陈玉柱
Owner 陈玉柱
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