Glycine diagnosis/measuring reagent kit and glycine concentration determination method
A technology of glycine and kits, which is applied in the field of medical/food inspection and determination, and can solve problems affecting health, nutritional imbalance, etc.
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Embodiment 1
[0044] The glycine diagnosis / measurement reagent of this embodiment is a single reagent, including:
[0045] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L
[0046] Stabilizer 500mmol / L
[0047] Coenzyme 3mmol / L
[0048] Hydrogen cyanide synthase 10000U / L
[0049] Formate dehydrogenase 12000U / L
[0050] NAD-dependent formate dehydrogenase 12000U / L
[0051] Phenazine methyl sulfate 0.4mmol / L
[0052] Ferrocytochrome B1 3mmol / L
[0053] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.
[0054] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the tested glycine sample to the reagent is 1 / 25, the reaction The direction is positive reaction (rising reaction), the delay time is abo...
Embodiment 2
[0057] The glycine diagnosis / measurement reagent of this embodiment is a double reagent, comprising:
[0058] Reagent 1
[0059] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L
[0060] Stabilizer 50mmol / L
[0061] Coenzyme 3mmol / L
[0062] Phenazine methyl sulfate 0.4mmol / L
[0063] Ferrocytochrome B1 3mmol / L
[0064] Reagent 2
[0065] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L
[0066] Stabilizer 500mmol / L
[0067] Hydrogen cyanide synthase 10000U / L
[0068] Formate dehydrogenase 12000U / L
[0069] NAD-dependent formate dehydrogenase 12000U / L
[0070] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.
[0071] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the tested glycine sample to reagent...
Embodiment 3
[0074] The glycine diagnosis / measurement reagent of this embodiment is three reagents, comprising:
[0075] Reagent 1
[0076] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L
[0077] Stabilizer 50mmol / L
[0078] Coenzyme 3mmol / L
[0079] Phenazine methyl sulfate 0.4mmol / L
[0080] Ferrocytochrome B1 3mmol / L
[0081] Reagent 2
[0082] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L
[0083] Stabilizer 500mmol / L
[0084] Formate dehydrogenase 12000U / L
[0085] NAD-dependent formate dehydrogenase 12000U / L
[0086] Reagent 3
[0087] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L
[0088] Stabilizer 500mmol / L
[0089] Hydrogen cyanide synthase 10000U / L
[0090] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.
[0091] When measuring the concentration of glycine, set on the automatic biochemical analyzer: temperature 37°...
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