Efficient hydrogen production functional gene carrier pet32a-fdhf and its construction and application

Abstract

The invention provides an efficient hydrogen-production functional gene vector pET32a-fdhF as well as construction and application thereof and relates to the technical field of genetic engineering. The gene vector pET32a-fdhF is a pET32a plasmid containing formate dehydrogenase fdhF genes of Bacillus cereus. The construction of the gene vector comprises the steps of amplifying segments of the formate dehydrogenase fdhF genes in Bacillus cereus by virtue of a design primer, linking the segments to a pET32a segment containing a corresponding linking terminal by virtue of recombinase, carrying out gene sequencing, and verifying the integrity of the genes. The pET32a-fdhF plasmid is converted into bacteria, and the bacteria are cultured in a hydrogen-production culture medium, so as to produce hydrogen. The pET32a-fdhF can be applied to different bacteria, so that the bacteria with no hydrogen-production capacity can produce hydrogen, the hydrogen-production of the bacteria capable of producing hydrogen is obviously improved, and more hydrogen can be rapidly produced by virtue of the same substrate concentration. According to the pET32a-fdhF, existing escherichia coli for industrial hydrogen production can be improved.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to a high-efficiency hydrogen production functional gene carrier pET32a-fdhF and its construction and application. Background technique [0002] With the depletion of fossil energy reserves such as coal and oil, it is urgent to develop new energy sources, and hydrogen energy has attracted much attention as a clean and high-heat energy source. Although hydrogen energy can be obtained through chemical methods, it is controversial because of its high cost and possible environmental pollution. Biological hydrogen production can solve this problem. There are many microorganisms for biohydrogen production, among which Escherichia coli hydrogen production is one of the best choices in terms of cost and efficiency. At present, wild Escherichia coli does not produce hydrogen or produces little hydrogen, which cannot meet human needs for energy. Therefore, genetic engineering or ...

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Problems solved by technology

At present, wild Escherichia coli does not produce hydrogen or produces little hydrogen, which cannot meet human needs for energy. Therefore, genetic engineering or metabolic engineering methods are used to transform Escherichia coli to greatly increase its hydrogen production performance

The existing hydrogen-producing Escherichia coli consumes a lot of substrates, but produces relatively little hydrogen, and the efficiency is relatively low. It is urgent to introduce exogenous genes through transformation to greatly improve the hydrogen production efficiency, and the hydrogen production efficiency of different exogenous genes also different

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