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Ammonia ion diagnosis/measuring reagent kit and ammonia ion concentration determination method

A kit and ion technology, which is applied in the field of ammonia diagnosis/determination kits and ammonia concentration determination, can solve the problem of undiscovered blood ammonia determination methods and the like

Inactive Publication Date: 2009-06-24
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Searching Chinese patents, only found 87105593.7 patent application which discloses a quick-frozen cup for measuring blood ammonia, but did not find an ideal method for measuring blood ammonia

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The ammonia (ion) diagnosis / measurement reagent of the present embodiment is a single reagent, comprising:

[0044] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0045] Stabilizer 500mmol / L

[0046] Reduced coenzyme 0.25mmol / L

[0047] Glutamine Synthetase 6000U / L

[0048] Phosphoenolpyruvate Carboxylase 8000U / L

[0049] Formate dehydrogenase 10000U / L

[0050] Glutamic acid 10mmol / L

[0051] Adenosine triphosphate 3mmol / L

[0052] Oxaloacetate 5mmol / L

[0053] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0054] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the ammonia (ion) sample to the reagent is 1 / 25, the reaction direction is negative reaction (down...

Embodiment 2

[0057] The ammonia (ion) diagnosis / measurement reagent of the present embodiment is a double reagent, comprising:

[0058] Reagent 1

[0059] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0060] Stabilizer 50mmol / L

[0061] Reduced coenzyme 0.25mmol / L

[0062] Glutamic acid 10mmol / L

[0063] Adenosine triphosphate 3mmol / L

[0064] Oxaloacetate 5mmol / L

[0065] Reagent 2

[0066] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0067] Stabilizer 500mmol / L

[0068] Glutamine Synthetase 6000U / L

[0069] Phosphoenolpyruvate Carboxylase 8000U / L

[0070] Formate dehydrogenase 10000U / L

[0071] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0072] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test subwavelength 405nm, tested ammonia (ion) samp...

Embodiment 3

[0075] The ammonia (ion) diagnosis / measurement reagent of the present embodiment is three reagents, comprising:

[0076] Reagent 1

[0077] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0078] Stabilizer 50mmol / L

[0079] Reduced coenzyme 0.25mmol / L

[0080] Glutamic acid 10mmol / L

[0081] Adenosine triphosphate 3mmol / L

[0082] Oxaloacetate 5mmol / L

[0083] Reagent 2

[0084] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0085] Stabilizer 500mmol / L

[0086] Phosphoenolpyruvate Carboxylase 8000U / L

[0087] Formate dehydrogenase 10000U / L

[0088] Reagent 3

[0089] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0090] Stabilizer 500mmol / L

[0091] Glutamine Synthetase 6000U / L

[0092] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.

[0093] When measuring the ammonia (ion) concentration, set it on the autom...

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Abstract

The invention relates to a kit for diagnosing / measuring ammonia (ions) by utilizing the technologies of the enzymic colorimetry and the enzyme linked immunosorbent assay. The invention further relates to a method, a principle and the composition and the components of a reagent for measuring the concentration of ammonia (ions), and belongs to the technical field of medical / food / environmental inspection and measurement. The main components of the kit include a buffer solution, reduced coenzyme, glutamic acid, adenyl pyrophosphate, oxaloacetic acid, glutamine synthetase, phosphoric acid enol pyruvate carboxylase, formic dehydrogenase and a stabilizer. Through mixing a sample and the reagent by a certain volume ratio, a series of enzymatic reactions occur, then the reactant is placed under an ultraviolet / visible light analyzer, and the degree / velocity of the decrease in absorbance at 340 nm of the dominant wavelength is detected, thereby measuring the concentration of ammonia (ions).

Description

technical field [0001] The invention relates to an ammonia (ion) diagnosis / measurement kit, and at the same time, the invention also relates to a method for measuring ammonia (ion) concentration, which belongs to the technical field of medicine / food / environmental inspection and measurement. Background technique [0002] Ammonia determination methods include micro-diffusion method, ion exchange method, enzymatic method and ammonia electrode method. The most widely used methods are enzymatic method and blood ammonia analyzer analysis method based on ion selective electrode. [0003] The diffusion method is that after the sample is alkalized, NH is released 3 , Titrate the released ammonia with acid, or use the Nessler reaction to form brown-yellow mercuric ammonium iodide for colorimetry. These methods need to be alkalized, and the endogenous ammonia formation will affect the accuracy and precision, so they are rarely used at present; the ion exchange method is more accurate...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N33/84C12Q1/00
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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