Kalium ion diagnosis/measuring reagent kit and kalium ion concentration determination method
A kit and ion technology, applied in the field of determination of potassium concentration, potassium diagnosis/measurement kit, can solve the problem of high electrode cost
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Embodiment 1
[0047] The potassium (ion) diagnosis / measurement reagent of the present embodiment is a single reagent, comprising:
[0048] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L
[0049] Stabilizer 500mmol / L
[0050] Reduced coenzyme 0.25mmol / L
[0051] Tryptophanase 6000U / L
[0052]Pyruvate dehydrogenase 8000U / L
[0053] Formate dehydrogenase 10000U / L
[0054] Tryptophan 10mmol / L
[0055] Ferricytochrome b1 3mmol / L
[0056] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.
[0057] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the tested potassium (ion) sample to the reagent is 1 / 25, the reaction direction is negative reaction (downward reaction), the delay time is about 2 ...
Embodiment 2
[0060] The potassium (ion) diagnosis / measurement reagent of the present embodiment is a double reagent, comprising:
[0061] Reagent 1
[0062] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L
[0063] Stabilizer 50mmol / L
[0064] Reduced coenzyme 0.25mmol / L
[0065] Tryptophan 10mmol / L
[0066] Ferricytochrome b1 3mmol / L
[0067] Reagent 2
[0068] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L
[0069] Stabilizer 500mmol / L
[0070] Tryptophanase 6000U / L
[0071] Pyruvate dehydrogenase 8000U / L
[0072] Formate dehydrogenase 10000U / L
[0073] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.
[0074] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test subwavelength 405nm, tested potassium (ion) sample and reagent 1, reagent 2 The volume ratio is...
Embodiment 3
[0077] The potassium (ion) diagnosis / measurement reagent of the present embodiment is three reagents, comprising:
[0078] Reagent 1
[0079] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L
[0080] Stabilizer 50mmol / L
[0081] Reduced coenzyme 0.25mmol / L
[0082] Tryptophan 10mmol / L
[0083] Ferricytochrome b1 3mmol / L
[0084] Reagent 2
[0085] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L
[0086] Stabilizer 500mmol / L
[0087] Pyruvate dehydrogenase 8000U / L
[0088] Formate dehydrogenase 10000U / L
[0089] Reagent 3
[0090] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L
[0091] Stabilizer 500mmol / L
[0092] Tryptophanase 6000U / L
[0093] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.
[0094] When measuring potassium (ion) concentration, set on the automatic biochemical analyzer: temperature 37°C, reaction tim...
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