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Kalium ion diagnosis/measuring reagent kit and kalium ion concentration determination method

A kit and ion technology, applied in the field of determination of potassium concentration, potassium diagnosis/measurement kit, can solve the problem of high electrode cost

Inactive Publication Date: 2009-06-24
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the ion selective electrode method is widely used, but the cost of the electrode is expensive.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] The potassium (ion) diagnosis / measurement reagent of the present embodiment is a single reagent, comprising:

[0047] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0048] Stabilizer 500mmol / L

[0049] Reduced coenzyme 0.25mmol / L

[0050] Tryptophanase 6000U / L

[0051] Pyruvate oxidase 8000U / L

[0052] Formate dehydrogenase 10000U / L

[0053]Tryptophan 10mmol / L

[0054] Coenzyme A 3mmol / L

[0055] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0056] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the tested potassium (ion) sample to the reagent is 1 / 25, the reaction direction is negative reaction (downward reaction), the delay time is about 2 minutes, and the...

Embodiment 2

[0059] The potassium (ion) diagnosis / measurement reagent of the present embodiment is a double reagent, comprising:

[0060] Reagent 1

[0061] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0062] Stabilizer 50mmol / L

[0063] Reduced coenzyme 0.25mmol / L

[0064] Tryptophan 10mmol / L

[0065] Coenzyme A 3mmol / L

[0066] Reagent 2

[0067] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0068] Stabilizer 500mmol / L

[0069] Tryptophanase 6000U / L

[0070] Pyruvate oxidase 8000U / L

[0071] Formate dehydrogenase 10000U / L

[0072] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0073] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test subwavelength 405nm, tested potassium (ion) sample and reagent 1, reagent 2 The volume ratio is 2 / 20 / 5, the r...

Embodiment 3

[0076] The potassium (ion) diagnosis / measurement reagent of the present embodiment is three reagents, comprising:

[0077] Reagent 1

[0078] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0079] Stabilizer 50mmol / L

[0080] Reduced coenzyme 0.25mmol / L

[0081] Tryptophan 10mmol / L

[0082] Coenzyme A 3mmol / L

[0083] Reagent 2

[0084] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0085] Stabilizer 500mmol / L

[0086] Pyruvate oxidase 8000U / L

[0087] Formate dehydrogenase 10000U / L

[0088] Reagent 3

[0089] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0090] Stabilizer 500mmol / L

[0091] Tryptophanase 6000U / L

[0092] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.

[0093] When measuring potassium (ion) concentration, set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, ...

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Abstract

The invention relates to a kit for diagnosing / measuring kalium (ions) by utilizing the technologies of the enzymic colorimetry and the enzyme linked immunosorbent assay. The invention further relates to a method, a principle and the composition and the components of a reagent for measuring the concentration of kalium (ions), and belongs to the technical field of medical / food / environmental inspection and measurement. The main components of the kit include a buffer solution, reduced coenzyme, tryptophan, coenzyme A, tryptophanase, pyruvate oxidase, formic dehydrogenase and a stabilizer. Through mixing a sample and the reagent by a certain volume ratio, a series of enzymatic reactions occur, then the reactant is placed under an ultraviolet / visible light analyzer, and the velocity of the decrease in absorbance at 340 nm of the dominant wavelength is detected, thereby measuring the concentration of kalium (ions).

Description

technical field [0001] The invention relates to a potassium (ion) diagnosis / measurement kit, and at the same time, the invention also relates to a method for measuring potassium (ion) concentration, which belongs to the technical field of medicine / food / environmental inspection and determination. Background technique [0002] Glomerular disease, renal insufficiency, diabetic ketosis, adrenal insufficiency, etc. When the serum potassium is as high as 7.5mmol / L or above, the patient will experience arrhythmia, and appropriate treatment should be considered. When serum potassium exceeds 10mmol / L, ventricular fibrillation can occur, cardiac arrest and death. High potassium stops the heart in diastole. [0003] At present, the commonly used methods for potassium determination are: isotope dilution mass spectrometry, neutron activation method, flame photometer method, chemical determination method, ion selective electrode method, atomic spectrophotometry, enzyme kinetics method. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N33/84C12Q1/00
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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