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Biological transformation and synthesis method of rubescensin

A synthesis method and oridonin technology, applied in the field of organic compound synthesis, can solve the problems of restricted development and utilization, complex chemical structure, difficulty in total synthesis, etc., and achieve the advantages of reducing production cost, protecting ecological environment and shortening cultivation time. Effect

Inactive Publication Date: 2011-11-16
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the content of such compounds in natural plants is very low, and the chemical structure is complex, and it is very difficult and uneconomical to use chemical methods for total synthesis.
This severely limits its further development and utilization

Method used

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  • Biological transformation and synthesis method of rubescensin

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1: A kind of biotransformation synthesis method of oridonin

[0050] A biotransformation synthesis method of oridonin of the present invention, the detailed steps of the synthesis method are as follows:

[0051] a. Preparation of solid medium:

[0052] The composition of the solid medium is: magnesium sulfate 360mg, potassium dihydrogen phosphate 170mg, potassium nitrate 1900mg, ammonium nitrate 1650mg, calcium chloride 440mg, thiamine 1.0mg, inositol 100mg, boric acid 6.2mg, manganese sulfate 15.6mg, sulfuric acid Zinc 8.6mg, sodium molybdate 0.025mg, copper sulfate 0.025mg, cobalt chloride 0.025mg, potassium iodide 0.085mg, ferrous sulfate 27.8mg, EDTA sodium salt (EDTA disodium) 37.3mg, 2,4-D 1.0mg, 6-furfurylaminopurine 2.0mg, naphthaleneacetic acid 3.0mg, sucrose 30000mg and agar 12000mg;

[0053] Weigh one by one according to the content of each component of the above-mentioned solid medium, fully dissolve other medium components except sucrose and ag...

Embodiment 2

[0056] Embodiment 2: basically the same as Embodiment 1, the difference is:

[0057] In step a: the composition of the solid medium is: magnesium sulfate 270mg, sodium dihydrogen phosphate 370mg, potassium nitrate 860mg, ammonium nitrate 4650mg, calcium chloride 180mg, thiamine 1.25mg, inositol 25mg, boric acid 25.8mg, manganese sulfate 62.5mg, zinc sulfate 2.1mg, sodium molybdate 0.0125mg, copper sulfate 0.0125mg, cobalt chloride 0.0725mg, potassium iodide 2.65mg, ferrous sulfate 65.5mg, EDTA sodium salt (EDTA disodium) 9.8mg, N 6 - Furanylmethyladenine 10mg, naphthoic acid 2.0mg, indole-3-acetic acid 15mg, sucrose 35000mg and agar 33000mg;

[0058] Weigh one by one according to the content of each component of the above-mentioned solid medium, fully dissolve other medium components except sucrose and agar with distilled water to obtain a solution, then add the weighed sucrose and agar into the prepared solution to dissolve, and Add water to dilute to 1000mL, adjust the pH v...

Embodiment 3

[0061] Embodiment 3: basically the same as Embodiment 1, the difference is:

[0062] In step a: the composition of the solid medium is: magnesium sulfate 160mg, potassium dihydrogen phosphate 70mg, potassium nitrate 4250mg, ammonium nitrate 725mg, calcium chloride 980mg, thiamine 0.05mg, inositol 250mg, boric acid 1.6mg, manganese sulfate 3.9mg, zinc sulfate 32.3mg, sodium molybdate 0.0725mg, copper sulfate 0.0725mg, cobalt chloride 0.0125mg, potassium iodide 0.0215mg, ferrous sulfate 6.5mg, EDTA sodium salt (EDTA disodium) 76.6mg, 6-benzyl Adenine 25mg, 4-amino-3,5,6-triclopyralid 55mg, sucrose 12000mg and agar 4500mg;

[0063] Weigh one by one according to the content of each component of the above-mentioned solid medium, fully dissolve other medium components except sucrose and agar with distilled water to obtain a solution, then add the weighed sucrose and agar into the prepared solution to dissolve, and Add water to dilute to 1000mL, adjust the pH value of the solution t...

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Abstract

The invention discloses a biological transformation and synthesis method of rubescensin. The synthesis method comprises the following steps of: firstly, preparing a solid culture medium and a liquid culture medium; then, sterilizing and then inoculating the leaves, leaf buds or rhizome shucks of rabdosia rubescens into the prepared solid culture medium for culturing; transferring the callus obtained by culturing into the liquid culture medium for culturing; extracting the callus cultured in the liquid culture medium by adopting an organic solvent; and removing the organic solvent after extracting to obtain a rubescensin product (kaurene type diterpenoid compound). In the invention, the technical scheme for culturing and preparing rubescensin is free from the restriction of natural resources and is favorable for protecting the ecological environment. The technical scheme disclosed by the invention is easy to realize industrialized production, has the advantages of shorter culture time and higher product yield, lowers the production cost, and has obvious economic and social benefits.

Description

technical field [0001] The invention relates to a synthesis method of organic compounds, in particular to a biotransformation synthesis method of oridonin-kaurene type diterpenoids. Background technique [0002] The scientific name of Dongling grass is broken rice, and the Latin name is Rabdosia rubsenst (Hamst), also known as Binglingcao, Yanmingcao, Caihuacao, etc. Donglingcao is a perennial herb or subshrub of Lamiaceae Fragrant Tea. It is mainly distributed in Taihang and Wangwu Mountains in Henan Province. Due to indiscriminate mining and excessive digging, its natural resources are nearly exhausted. [0003] The Cordyceps japonicus plant contains monoterpenes, sesquiterpenes, diterpenes, and triterpenes, as well as various trace elements such as Fe, Ca, Zn, Se, and various amino acids such as alanine and aspartic acid. Composition, Rubescensin includes Rubescensin A (rubescenin A=oridonin), Rubescensin B=ponicidin, Rubescensin C (rubescensin C) and Rubescensin (pube...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/18
Inventor 吴鸣建赵天增马川沈国鹏刘冰
Owner ZHENGZHOU UNIV
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