171 results about "Adenosine Phosphates" patented technology

The invention is directed to compositions and methods which permit the oral use of adenosine and adenosine phosphates for cardiovascular applications such as pulmonary artery hypertension, cardiac failure and other diseases. Certain compositions in accordance with the invention have enhanced AMP gastrointestinal bioavailability and thus efficacy. The invention prolongs the activity of adenosine and adenosine phosphates when administered intravenously. In particular, the invention contemplates methods of treating several human (as well as animal) cardiovascular and neurological medical conditions that could be improved by an effective amount of adenosine, ATP or AMP combined with dialkylaminoalcohols and their salts.

The invention discloses a creatine jubase MB isozyme activity detection reagent which comprises sulfide-oxidizing coenzyme, 6-phosphaogluconate dehydrogenase, sulfhydryl reagent, magnesium salt, glucokinase or hexoxinase, anti-CK-M antibody, phosphocreatine, adenosine diphosphate, glucose and glucose-6-phosphate dehydrogenase. The preparation method of the reagent is as follows: dissolving all components in a buffer solution with pH of 5.0 to 9.5. The creatine jubase MB isozyme activity detection reagent can enlarge detection sensitivity several times; and the determination result has high precision and degree of accuracy.

The invention relates to a cell strain for measuring the bioactivity of GLP-1 and functional analogue thereof and an application of the cell strain and belongs to the technical field of measurement of drug bioactivity. The GLP-1R / HEK293 cell strain is preserved in the common microorganism center of China microorganism culture preservation management committee and the preservation number is CGMCCNo.5909. The invention relates to a measuring method for establishing express glucagon peptide-1 receptor (GLP-1R) genetic engineering cell strain GLP-1R / HEK293 and measuring the bioactivity of GLP-1 and analogue thereof in external stimulation on the basis of the change in the cAMP level in the cell after the GLP-1R / HEK293 cell strain is stimulated by the glucagon peptide-1 receptor (GLP-1R) and the functional analogue thereof. The measuring method is easily standardized, is excellent in repeatability and has the characteristics of low cost, convenience and accuracy, thereby being excellent in application prospect.

The invention relates to a freshness detecting method used for royal jelly. In the specific detecting method, the contents of adenosine triphosphate (ATP), adenosine diphosphate (ADP), single phosphoric acid adenosine (AMP), inosinic acid (IMP), hypoxanthine riboside (HxR), hypoxanthine (Hx), adenine and adenosine of the royal jelly are quantitatively determined through methods of liquid chromatography or ultraviolet spectrometry, and the like; the ratio (F value) between the accumulation amount of adenine, adenosine, HxR and Hx and the sum of the qualities of the eight substances (degradation products of ATP and nucleic acid)is calculated; the F value of the royal jelly is used for detecting the freshness of the royal jelly and judging the quality of the royal jelly. Compared with a present royal jelly quality detecting method, the freshness detecting method can judge the quality and the freshness of the royal jelly more accurately and sensitively, can improve the quality standard of the present royal jelly and effectively monitor the quality of the royal jelly.

The invention provides adenylate cyclase, and a coding gene, a vector, a bacterial strain and application thereof. A DNA (Deoxyribose Nucleic Acid) sequence for coding the adenylate cyclase provided by the invention has a base sequence represented by SEQ ID NO.:1. Furthermore, the invention further provides the adenylate cyclase coded by the DAN sequence, a recombinant vector including the DAN sequence, a host cell including the recombinant vector, and applications of all in production of the adenylate cyclase. The genetic engineering strain of colibacillus, disclosed by the invention, can beused for expressing the adenylate cyclase with high efficiency. The inducible enzyme activation thereof can achieve 7 U / mg or 10 U / mg. The bacterial strain is used for producing cyclic adenosine monophosphate and has the advantages of simple process, moderate condition, short cycle, few by-products and the like.

The invention belongs to the field of insecticides, and relates to an insecticidal and acaricidal composition containing an ATP synthetase inhibitor acaricide. The composition contains an active component A and an active component B, a weight part ratio of the component A to the component B is 1:99-99:1, the active component A is a compound I represented by a structural formula shown in the invention, and the active component B is selected from a mitochondrial adenosine triphosphate (ATP) synthetase inhibitor acaricide. The composition has the advantages of obvious synergy and resistance delaying, and can be used for controlling various insects.

Compositions comprising the nucleotides adenosine diphosphate and adenosine monophosphate, and their use for neutralizing effects of excess incident energy on the human body, particularly effects of ionizing radiation from nuclear or radiological energy sources, are disclosed and claimed.

The invention discloses preparation and application of an Mxene-Mn3(PO4)2 composite electrode material. The preparation is characterized in that two-dimensional Ti3C2 nanosheets are synthesized from aTi3AlC2 ceramic material as a raw material, and ATP (adenosine triphosphate) is used as a template to induce synthesis of Mn3(PO4)2 nanoparticles. The Ti3C2 / Mn3(PO4)2 nanocomposite synthesized with the method integrates good conductivity of Ti3C2 and biological mimetic enzyme activity of Mn3(PO4)2, and the composite material has excellent conductivity and catalytic performance by complementary advantages of the two raw materials, and is the excellent electrode material for preparing superoxide dismutase. The composite material can be used for constructing biosensors and has excellent application prospects in the fields of medical diagnosis, medical research and the like.

The invention relates to a kit and a method for determining triglyceride. The kit comprises a reagent group 1 and a reagent group 2, wherein the reagent group 1 comprises magnesium salts, triphosadenine, glycerokinase, glycerol lipase oxidase, peroxidase and chromogen; the reagent group 2 comprises lipoprotein lipases and 4-amino-antipyrine, wherein the chromogen is N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethoxyaniline (DAOS) or comprises DAOS. The kit and the method provided by the invention have the advantage that the interference caused by calcium dobesilate and / or etamsylate existing in the sample during the triglyceride detection can be reduced.

The invention relates to a method for extracting red date cyclic adenosine monophosphate through an ultrasonic composite membrane technology. The method solves the problem that the existing extraction technology has the defects of complex processes, low extraction rate, high industrialization difficulty and low product purity. The method comprises the following steps of removing date pits of cleaned red dates, crushing the red dates obtained by the previous step, adding an appropriate amount of an ethanol solvent into the crushed red dates, carrying out ultrasonic reinforcement extraction, repeatedly carrying out ultrasonic extraction of extracted filter residues, merging the extract obtained by the previous step, removing macromolecules of bacteria, proteins, pectin, polysaccharides and starch, and monosaccharides of glucose and fructose respectively through ultrafiltration and nanofiltration, intercepting cyclic adenosine monophosphate-rich concentrate, and carrying out pressure reduction condensation and spray drying to obtain 30% of a powdery cyclic adenosine monophosphate crude product. The method provided by the invention is easy for operation, is convenient for extraction, is fast, has short extraction time and a high cyclic adenosine monophosphate extraction rate, and is suitable for industrialized production. A product obtained by the method has high purity, is purely natural and does not produce chemical pollution. Therefore, the method provides an effective approach for comprehensive development of red date further processing.

The invention discloses a method for preparing low molecular weight heparin with high activity, which is characterized in that the prior low molecular weight heparin with low anticoagulant activity is used as a substrate, a PAPS (3'-phosphoadenosine-5'-phosphoric acid sulfuric acid) regeneration system is adopted, and a heparin biosynthetic enzyme 3-O-sulfate-transferase is used for modifying the substrate to obtain the low molecular weight heparin with high anticoagulant activity; and the PAPS regeneration system can use extremely cheap PNPS (p-nitryl potassium phenolsulfonate) as an enzyme-modified reactive sulfate donor to further save the cost and make the industrialization possible. The method adopts the 3-O-sulfate-transferase with high enzyme activity to combine with the PAPS regeneration system, performs selective modification on the low molecular weight heparin, and increases the number of anticoagulant activity centers so as to greatly improve the anti-thrombosis activity of the heparin. The method provides a path for the enzymatic industrial production of the low molecular weight heparin with high activity.

The invention relates to control of biofilm development. Specifically, some embodiments of the present invention relate to control of bacterial biofilm formation through addition or breakdown of signal(s) that induce biofilm formation. More specifically, some embodiments of the present invention relate to control (e.g., promotion, prevention) of biofilm development by application or hydrolysis of adenosine triphospate (ATP), deoxyadenosine triphosphate (dATP), or derivatives or analogs thereof (e.g., through application or administration of an agent that hydrolyzes ATP, dATP, or derivatives or analogs thereof (e.g., apyrase)).

The invention provides a stable compound coenzyme preparation and a preparation method thereof. The main compositions and proportions of the stable compound coenzyme preparation are as follows: 90-120 U of coenzyme A (CoA), 0.1-0.3 mg / ml coenzyme I (NAD), 1-5 mg / ml glutathione (GSH), 1-2.5 mg / ml adenosine triphosphate (ATP), 1.8-12 mu g / ml flavin mononucleotide (FMN), 3-13 mu g / ml flavin adenine dinucleotide (FAD), 0.1-0.4 mg / ml adenosine diphosphate (ADP), 0.2-0.4 mg / ml adenosine monophosphate (AMP), 1.2-15 mu g / ml of adenosine methionine (SAM), 1-5 mg / ml calcium gluconate, 0.5-1.5 mg / ml cysteine hydrochloride, and 0.6-5 mg / ml mannitol. The preparation method overcomes the defects that high-speed centrifugalization is adopted, freeze-drying conditions are not clear and the product stability is poor in the existing method, and the stable compound coenzyme preparation is widely applied to the practices of preparing drugs for treating cardia-cerebrovascular diseases and digestive system diseases.

The invention discloses a nucleotide probiotic capsule for delaying senescence, and belongs to the technical field of medicine and health care. The nucleotide probiotic capsule comprises a capsule shell and a content, wherein the content comprises four exogenous nucleotides and five probiotics; the exogenous nucleotides comprise 5'-cytidine monophosphate (5'-CMP), 5'-adenosine monophosphate (5'-AMP), 5'-disodium uridine monophosphate (5'-UMPNa2) and 5'-disodium guanylate (5'-GMPNa2); the probiotics comprise lactobacillus acidophilus, lactobacillus plantarum, lactobacillus casei and the like; and the content of the probiotics is greater than 1 * 10 <7>CFU / g. The nucleotide probiotic capsule for delaying senescence can remove free radicals in a body, inhibit oxidative damage of the free radicals to the body and prolong the cell life, has multiple health care effects of delaying senescence, prolonging life, improving immunity, regulating intestinal flora and the like, is convenient to carry and take, and is a health care food suitable for middle-aged and elderly people.

The solid disodium adenosine triphosphate composition for injection consists of disodium adenosine triphosphate 25.0-50.0 wt%, magnesium chloride 8.0-16.0 wt%, sugar alcohol as stabilizer 0.5-50.0 wt% and amino acid as buffering agent 0.5-50.0 wt%, with the weight ratio between disodium adenosine triphosphate and magnesium chloride being 100 to 32. the solid composition has high stability, high solubility, convenient clinical application, and raised clinical application safety of disodium adenosine triphosphate-magnesium chloride injection.

The invention provides a milk powder of an immune formula. Each 100g of milk powder comprises the following components: 20-50g of a raw milk extract, 30-55g of demineralized whey powder, 5-20g of vegetable oil, 0.5-6g of probiotics, 20-100mg of inositol, 10-60mg of taurine, 8-50mg of L-carnitine and / or salt of L-carnitine, 50-150mg of choline matters and 15-45mg of nucleotide, wherein a weight ratio of five nucleotides including guanosine 5'-monophosphate disodium salt, cytidine 5'-monophosphate disodium salt, uridine-5'-monophosphate disodium salt, adenosine 5'-monophosphate disodium salt and guanosine 5'-monophosphate disodium salt is 1 to (0.1-10) to (1-5) to (0.1-5) to (0.1-3). The milk powder is relatively reasonable in formula ratio, and provides relatively complete nutrients and good absorption for infants, and the immunity of the infants is completely improved; meanwhile, components of maltodextrin, saccharose or white granulated sugar, essence, a pigment, an emulsifying agent and the like are not added, and thus the health of the infants is relatively facilitated.

The invention discloses recombinant escherichia coli for high yield of cytidine monophosphate and application of the recombinant escherichia coli. According to the invention, cytidine kinase gene is obtained by cloning from a cytidine monophosphate production strain; crude enzyme liquid obtained by crushing recombinant bacteria has good catalytic activity and stability; cytidine, adenosine triphosphate (ATP) and Mg < 2 + > are added, the reaction system is simple, the conditions are mild, the period is short, byproducts are fewew, the method is clean and free of pollution, the application is asimple, rapid and efficient production way, and the conversion rate of substrate cytidine and ATP reaches 85% or above.

The invention encompasses compositions and methods for effectively treating and / or preventing diabetes and / or obesity. This is accomplished by totally addressing the multiple mechanisms that lead to such conditions. The invention includes compositions comprising a combination of agents that effectively suppress, regulate or interfere with the various biochemical processes and mechanisms that lead to diabetes and obesity. The inventive compositions used for administration to human and other mammalian subjects comprise (1) at least one agent capable of modulating expression and / or activity of one or more of peroxisome activated protein receptor gamma (PPAR-γ), CAAT / enhancer binding protein-α (C / EBPα) and Sterol Regulatory Element-Binding Protein (SREBP-1); (2) at least one agent capable of activating Wnt / β-catenin pathway; (3) at least one agent capable of activating the adenosine monophosphate-activated protein kinase (AMPK) signaling pathway; (4) at least one agent that inhibits the activity of pro-oxidants including reactive nitrogen species and reactive oxygen species (ROS); (5) at least one agent that suppresses one or more of inflammatory mediators including interleukins IL-1α, IL-1β, IL-6, NF-κB, TNF-α, matrix metalloproteinases (MMPs) and prostaglandin E2 (PGE2); (6) at least one agent capable of enhancing glucose transporter (GLUT4) and / or inhibiting glucose transporter GLUT2; (7) at least one agent that induces the expression of and / or activates adiponectin and (8) at least one agent that induces the expression of and / or activates sirtuin (SIRT1). The active agents for use herein are natural materials such as phytonutrients, vitamins and minerals. Compositions with combinations of such natural agents have the ability to prevent, reduce or treat diabetes and obesity by (a) clearing glucose and fatty acids from blood, (b) reducing the number of adipose cells and fat storage, (c) interfering with fat, glucose, and cholesterol biosynthesis, and (d) promoting fat and glucose oxidation.Since the present compositions are aimed toward normalizing metabolism and energy expenditure and managing oxidative stress and inflammation, they are also beneficial in relation to physical activity, in particular performance, endurance, fatigue and recovery during intensive and continuous exercise / exertion.

The invention discloses a specific marking method for 5-hydroxymethyluracil on DNA (deoxyribonucleic acid) and belongs to the technical field of molecular biology. The specific marking method comprises the following steps: transferring gamma-thiophosphate to 5-hmU from 5-O-thiotriphosadenine by using 5-hmU separated from bacteriophage M6 of pseudomonas aeruginosa so as to generate 5-thiophosphoricacid methyluracil, representing the generated 5-thiophosphoric acid methyluracil by using a liquid chromatogram-tandem mass spectrometry method, and marking sulfydryl through crosslinking chemistry,so as to achieve specific marking on 5-hydroxymethyluracil on DNA. By adopting the method disclosed by the invention, defects that a great amount of detection samples are needed, chemical reaction conditions are harsh, the reaction efficiency is low, and the like, can be effectively overcome.

The invention relates to a disodium adenosine triphosphate troche medical composition, and in particular relates to use of calcium salt in preparation of stable triphosadenine or a solid medical composition of officinal salt thereof. The medical composition provided by the invention further stably comprises triphosadenine or the solid medical composition of officinal salt thereof. More specifically, the invention relates to the solid medical composition containing triphosadenine or the officinal salt thereof, wherein even if the medical composition is stored for a long time, the content of the effective components is still highly maintained to ensure the excellent stability. After the solid medical composition provided by the invention is placed at 20+ / -2 DEG C for 2 years, the residual ratio of active components, i.e., triphosadenine or officinal salt thereof in the composition is over 80%.

The invention relates to preparation and application of ATP (adenosine triphosphate) near-infrared nanometer fluorescence probe. The fluorescence probe is structurally formed by nano-level zeolite imidazole frameworks (ZIF-90) and rhodamine-based near-infrared fluorophores coated inside the nano-level zeolite imidazole frameworks. The invention provides a preparation method for synthesizing the fluorescence probe by using 2-(4-diethylamino-2-hydroxybenzoyl) benzoic acid, trimethyl-2-indoline, zinc acetate dehydrate, imidazole-2-formaldehyde and the like as raw materials. The fluorescence probeis an ATP near-infrared fluorescence probe based on ZIF-90. Firstly, the fluorescence probe is simple in synthesis method and can generate 72 times of fluorescence enhancement for ATP; secondly, thefluorescence probe has relatively ideal selectivity for the ATP and cannot be obviously interfered by common ions in other nucleotides and living bodies; moreover, the fluorescence probe is rapid to react with the ATP, and response time is within 400s; and in addition, the fluorescence probe has long near-infrared emission, and is applied to detection on ATP content in living cells.

The invention discloses a tablet candy taking red date cyclic adenosine monophosphate as a functional component. The tablet candy comprises the following raw material components: 10-80 parts of cyclic adenosine monophosphate concentrated powder, 10-50 parts of glucose, 20-30 parts of lactose, 20-40 parts of skim milk powder, 1-15 parts of citric acid and 0.1-2 parts of polyethylene glycol 6000; the tablet candy is prepared by the steps of respectively grinding all the raw materials, screening the raw materials by a 80-mesh sieve, evenly mixing the raw materials, making soft material, pelleting, drying, finishing granules and tabletting to obtain the tablet candy taking the red date cyclic adenosine monophosphate as the functional component; the content of the cyclic adenosine monophosphate in the candy is 1-15mg; the functional component in the product takes part in substance metabolism in cells, and has the functions of inhibiting the formation and the proliferation of cancer cells, protecting the cardiac function, improving the sleep, improving the memory, improving anemia, relieving the climacteric syndrome, increasing immunity, etc.

Provided is a method for quantifying a target substance typified by amino acid. This method includes: a step in which an enzyme capable of converting the target substance and also of generating a pyrophosphate using an adenosine tri-phosphate (ATP) as a base material therefor is caused to act on the target substance, and pyrophosphate is generated; a step in which a pyruvate phosphate dikinase (PPDK) is caused to act on the generated pyrophosphate, in the presence of adenosine monophosphate (AMP) and phosphoenolpyruvic acid (PEP), and ATP, phosphoric acid, and pyruvic acid is generated; and a step in which the generated pyruvic acid is quantified. The amount of target substance is then determined on the basis of the obtained pyruvic acid amount. As a result of this invention, amino acid in a sample derived from an organism including a large quantity of a variety of impurities such as inorganic phosphoric acid and urea can be readily and quickly quantified without being affected by the impurities.

The present invention relates to the field of fuel additives, in particular, to an additive for hydrocarbon fuels to enhance efficiency and / or reduce pollution. The fuel additive of the invention includes an adenosine phosphate. The use of the adenosine phosphate results in enhanced combustion when combined with hydrocarbon fuel and combusted.

The invention belongs to the technical field of application of visual recognition of small biological molecules, and particularly relates to preparation of a novel pincer-like Zn(II) metal organic complex and application of the pincer-like Zn(II) metal organic complex to naked-eye visual recognition of triphosadenine. The expression of the pincer-like Zn(II) metal organic complex is NNN-Zn-(NO3)2, wherein NNN represents a terpyridyl ligand coordinated with Zn. Under a simple operation (heating / cooling) condition, when the pincer-like Zn(II) metal organic complex 1 is mixed with 0.25 molar equivalent of triphosadenine in water, a kind of two-component metal hydrogel can be formed through the conventional get preparation operations of heating and cooling. While when the pincer-like Zn(II) metal organic complex 1 is mixed with 0.25 molar equivalent of adenosine diphosphate and adenosine monophosphate in water, no metal hydrogel but sediments can be generated through the conventional gel preparation operations such as heating and cooling. The invention develops a fast, simple and convenient new method for naked-eye visual recognition of triphosadenine for the first time.

The invention discloses Bishutong nasal drops for children. The Bishutong nasal drops are prepared by combining a liquid A with a liquid B which have equal volume, wherein the liquid A comprises raw materials in percentage by mass as follows: 3%-7% of houttuynia cordata, 0.5%-1.5% of bupleurum chinense, 0.5%-1.5% of biond magnolia flowers, 0.005%-0.008% of naphazoline, 0.8%-1% of sodium chloride, 0.8%-1.2% of methylparaben and 87.792%-94.395% of deionized water; the liquid B comprises raw materials in percentage by mass as follows: 3%-7% of houttuynia cordata, 0.04%-0.06% of chlorpheniramine, 0.008%-0.012% of dexamethasone, 0.08%-0.12% of adenosine triphosphate, 0.08%-0.13% of lysozyme, 0.8%-1% of sodium chloride, 0.8%-1.2% of methylparaben and 90.478%-95.192% of deionized water. The Bishutong nasal drops for children has reliable efficacy when treating rhinitis and sinusitis of children and is convenient to use, and the clinical total effective rate is 100%.

The invention discloses biomarker compositions, and provides biomarker compositions for diagnosing cervicitis and a cervical cancer. The biomarker compositions are characterized by at least comprising one or a combination of uridine-5'-monophosphate disodium, histamine, 3'-O-methyl-guanosine and phenyllactic acid. The biomarker composition for diagnosing the cervicitis is characterized by comprising one or a combination of uridine-5'-monophosphate disodium, L-cysteine, isocitric acid, uridine diphosphate glucose, adenosine monophosphate, inosine 5'-monophosphate, L-pipecolic acid, N-acetyl putrescine and saccharose. The biomarker composition for diagnosing the cervical cancer is characterized by comprising one or a combination of histamine, phenyllactic acid, citraconic acid, L-2-aminoadipic acid, 3'-O-methyl-guanosine, beta-D-glucosamine, glycerin and cholesterol sulfate.

The invention discloses a natural medicine composition for treating involutional depression and non-classical depression and use of the natural medicine composition, and belongs to the technical field of research of natural medicine formulas in compatibility dosage effect and use of the natural medicine. The composition consists of the components in percentage by mass: 70%-97.5% of hippophae rhamnoides fruit oil and 2.5%-30% of pseudo-ginseng stem and leaf total saponins. The natural medicine composition disclosed by the invention is reasonable in compatibility and safe in dosage, is free from any side effects on human bodies, can solve the problems that a conventional medicine has side effects and poor treatment effects when being used for treating climacterium and involutional depression, and has a significant regulation effect on serum marker indexes, namely adenylate cyclase-cyclic adenosine monophosphate and cyclic guanosine monophosphate, and reproductive hormonesm and target acceptors of various impact factors, such as follicular generation promoting hormones, pitocin, dihydrotestosterone hormones, female hormones, adrenocorticotropin hormones, cytokine interleukin-6, interleukin-8 cytokine and interleukin-2, so as to achieve a treatment effect on treatment of the involutional depression and the non-classical depression diseases.