The invention belongs to the technical field of sealants, and particularly relates to a silane-blocked polyether sealant and a preparation method and application thereof. The sealant is prepared from,by mass, 10-35% of silane-blocked polyether, 10-30% of a plasticizer, 45-60% of solid powder, 1.0-2.5% of a water removal agent, 0.5-2.0% of a coupling agent, 0.5-3.0% of an accessory additive and 0.05-1.0% of a catalyst. The silane-blocked polyether sealant prepared by using the method is uniformly dispersed, moderate in strength, high in elongation rate, high in elastic recovery rate, excellentin adhesion, environmentally friendly, high in biocompatibility and capable of meeting market requirements in the assembly type construction industry.