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55results about How to "Meet the requirements of the experiment" patented technology
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Stabilized-pressure oil supply system of high-pressure direct-injection injector and control method thereof
InactiveCN101963120AOil pressure fluctuation is smallMeet the requirements of the experimentElectrical controlEngine testingFuel tankHigh pressure
The invention provides a stabilized-pressure oil supply system of a high-pressure direct-injection injector and a control method thereof. The stabilized-pressure oil supply system mainly comprises a high-pressure nitrogen cylinder, a pressure reducer, a step motor, a pressure sensor A, a two-position three-way electromagnetic valve A, an accumulator, a two-position three-way electromagnetic valve B, a pressure sensor B, an injector, a check valve, a fuel pump, an oil tank, a controller and a display screen. The stabilized-pressure oil supply system can automatically regulate the injection pressure of the injector of the stabilized-pressure oil supply system to a set injection pressure through the controller of the stabilized-pressure oil supply system according to the pressure parameter set on the display screen and can reduce the oil pressure fluctuation in the injection of the injector. The stabilized-pressure oil supply system has an accumulator oil supply path, so the fuel supply to the accumulator can be realized without detaching the pipe.
Owner:BEIHANG UNIV
Multifunctional adjustable monkey chair
InactiveCN101249026AFacilitate surgeryEasy to operateAnimal fetteringEngineeringScientific experiment
The invention discloses a multifunctional adjustable monkey chair, comprising a chair space surrounded by a lateral plate, a front plate and a back plate and allowing an experimental animal entering inside, and an upper plate and a head fixing plate installed above the chair space, wherein a plurality of fixing grooves are installed on the inner side of the lateral plate, and a groove is opened on the inner side of the fixing groove for installing matched plug-in units; and a rotary fixed element is installed on the outer side of the lateral plate and connected with a positioning rack for adjusting the position of the positioning rack. The monkey chair can be adjusted according to experimental conditions; and is suitable for scientific experiments on primates with different ages and shapes. The inventive monkey chair has the advantages of good commonality and convenient installation.
Owner:INSITUTE OF BIOPHYSICS CHINESE ACADEMY OF SCIENCES +1
Whole body exposure system for animals
InactiveCN104367398AAccurate concentrationRealize automatic controlVeterinary instrumentsAutomatic controlInhalation
The invention provides a whole body exposure system for animals. The whole body exposure system comprises a whole body exposure chamber, an aerosol generation system, a sampling system, a waste gas recycling and treating system and a control system; the whole body exposure chamber is used for placement of animals for toxicity experiment; the aerosol generation system is communicated with the whole body exposure chamber and used for generating aerosol for inhalation toxicity experiment; the sampling system is used for collecting concentration of the aerosol in the whole body exposure chamber; the waste gas recycling and treating system is communicated with the whole body exposure chamber and used for recycling and treating waste gas generated by the animals and excessive aerosol; the control system is used for regulating the concentration of the aerosol in the whole body exposure chamber in the experiment process. The concentration of the aerosol in the whole body exposure chamber is detected through the control system and the sampling system, automatic control of the whole body exposure system is realized, accurate control on the concentration of the aerosol in the whole body exposure chamber is realized, and requirements of experiments are met. The whole body exposure system with the automatic control system is high in automation degree and experiment accuracy.
Owner:BEIJING HUIRONGHE TECH
Device for measuring flow of flap gap based on PIV method
ActiveCN107748052AMeet the requirements of the experimentGuaranteed accuracyAerodynamic testingBeam splittingOptoelectronics
The invention belongs to the technical field of wind tunnel tests, and in particular relates to a device and method for measuring the flow of a flap gap based on a PIV method. The method comprises thesteps of emitting a laser sheet from an emitting end of a laser, and dividing the laser sheet into two laser sheets through a light path device; coarsely tuning reflectors in the light path device and making the two laser sheets radiating respectively from two directions into a flap gap; and finely tuning the reflectors and making the two laser sheets overlap, that is, completing the constructionof a light path. The device includes a beam splitting cube, reflectors, and an optical clamping member. The device also includes a PIV unit. The device is simple and practical in structural principle, well solves the problem of light path limitation on the PIV shooting of a flow field like a flap gap environment, and greatly improves the efficiency of PIV shooting.
Owner:NANJING UNIV OF AERONAUTICS & ASTRONAUTICS
Method for separating and culturing mouse synovial cells
InactiveCN107475179AHigh purityA large amountCell dissociation methodsArtificial cell constructsBALB/cSynovial Cell
The invention provides a method for isolating and cultivating mouse cells, which includes the following steps: (1) sterilizing tissue processing equipment; (2) selecting male BALB / c mice aged 1 to 2 months, killing them by breaking their necks, and using surgical scissors. Separate the synovial layer and fibrous layer of the joint capsule, then remove the synovial layer tissue, put the tissue into pre-cooled sterile PBS solution containing double antibody and wash it several times to remove blood stains and fatty tissue; (3) Cut the tissue into pieces, and use Digest with preheated type II collagenase and trypsin respectively; (4) Stop digestion, centrifuge, and discard the supernatant; (5) Inoculate into the coated culture flask by manual adhesion method; (6) After incubation for 1 to 2 hours, Cultivate in a 37°C, 5% CO2 environment; (7) Cell morphology observation; (8) Cell subculture; (9) Cell identification. The method for isolating and culturing mouse synovial cells provided by the invention is simple to operate, has a large number of cells obtained, and has a high survival rate. It is an ideal primary method for isolating and cultivating mouse synovial cells and provides reliable results for experiments. Cell resources.
Owner:JIANGYIN CHI SCI
Multi-concentration animal oral-nasal inhalation dynamic exposure equipment
ActiveCN104374928AImprove control accuracy and automationPrevent poisonous gas leakagePreparing sample for investigationEngineeringStreamflow
The invention discloses a multi-concentration animal oral-nasal inhalation dynamic exposure equipment which comprises an air compressor, an aerosol generation system, an oral-nasal exposure unit and a waste gas recovery treatment system, wherein the oral-nasal exposure unit comprises an experiment group exposure chamber; the aerosol generation system is connected with an aerosol inlet of the experiment group exposure chamber; a waste gas outlet of the experiment group exposure chamber is connected with the waste gas recovery treatment system; the equipment also comprises a multi-concentration dilution system; the experiment group exposure chamber comprises multiple sections of experiment exposure chambers; and the multi-concentration dilution system comprises a second aerosol diluter arranged among the sections of experiment exposure chambers and a third air flow controller for controlling the air inflow in the second aerosol diluter. The second aerosol diluter is arranged among the sections of experiment exposure chambers and used for diluting the aerosol entering the second section of experiment exposure chamber from the first section of experiment exposure chamber so as to meet the experiment requirements and perform experiment comparison in different concentration conditions.
Owner:BEIJING HUIRONGHE TECH
Simulation device and simulation method for simplified calculation of ship-ice collision in water medium
ActiveCN108750002ATo achieve the purpose of decouplingExact load inputVessel designingBody shapeImpact velocity
The invention discloses a simulation device for simplified calculation of ship-ice collision in a water medium which comprises a water tank, wherein a lifting support frame is arranged in the water tank; an ice body model is arranged on the support frame; a ship model is arranged in the water tank; a guide rail is arranged on the water tank; a towing device is arranged on the guide rail; the towing device is connected with the ship model through a towing truss, a pressure sensor is arranged at the top end of the ship, and the pressure sensor is sequentially connected with a charge amplifier, amulti-channel signal collector and a computer; the ship collides with the ice body model through the driving of the towing device so as to acquire the collision data through the pressure sensor. On the premise of not using ice material, the device can carry out high-pressure loading with different ice body shapes, ice body masses, impact speeds, impact positions, ship model floating states and impact angles, the real situation of the collision between the ship and the ice can be reflected well, the cost is low, the operation is easy, and the risk is small.
Owner:JIANGSU YANGZI XINFU SHIPBUILDING CO LTD
Experiment platform and construction method thereof
InactiveCN101996502ASmooth connectionIncrease contrastEducational modelsMechanical appliancesEngineeringComputer experiment
The invention discloses an experiment platform and a construction method thereof, relating to teaching experiment technique, method and product, in particular to an experiment platform taking culturing student comprehensiveness, designability, innovation and practical manipulative ability as the target. The experiment platform comprises an experiment hanging board (1) and an experiment hanging piece (2), wherein the experiment hanging piece (2) is articulated with the experiment hanging board (1) in an articulation mode. The experiment platform also can comprise a waste and old material basic experiment condition base (3), a mated experiment condition base (4), an operating floor (5), a computer experiment application system (6) and a typical experiment platform (8). By using such experiment platform, students can choose experiment articles for use like going to the supermarket, construct the expected experiment platform like writing and drawing on the blackboard and approach the real whole flow practice training like researching, designing and manufacturing new products in the scientific research and production practice, which is not only beneficial to improving the experiment effect of students and saving the experiment funds but also is beneficial to promoting the effective utilization of waste and old electromechanical equipment, and therefore, the goals of saving the resources and protecting the environment are achieved.
Owner:苏承慧
Pond field experimentation device and using method thereof
InactiveCN103210873AGuaranteed independencePrevent escapePisciculture and aquariaEcologic studyAeration
The invention discloses a pond field experimentation device and a using method thereof and relates to a device for experimental study of pond water area ecology and a using method thereof. The problems that the water oxygenation effect is bad, the water inlet and drainage are inconvenient, fishes are difficult to escape and the requirements of experimental study of pond water area ecology cannot be met in the conventional field experimentation enclosure ecosystem are solved. The pond field experimentation device comprises waterproof cloth, a U-shaped pipe, an oxygenation aeration device, an anti-escape net, two filtering nets, two straight pipes, four upright rods and four transverse rods, wherein the four upright rods are arranged in an array; a cross rod is connected between two upright rods; and the four upright rods and four transverse rods form a square frame. The field enclosure experimentation method mainly comprises the following steps: 1, inserting the upright rods on the square frame into the bottom of the pond; 2, injecting water into the square frame with the waterproof cloth; 3, oxygenating and aerating in water; and 4, draining water in the square frame. The pond field experimentation device is used for research of pond water area ecology.
Owner:HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
Method for separating and culturing primary human esophageal epithelial cells
InactiveCN108018258AHigh purityA large amountCell dissociation methodsEpidermal cells/skin cellsBottlePre cooling
The invention provides a method for separating and culturing primary human esophageal epithelial cells. The method comprises the following steps: (1) aseptically taking out 2 cm of a normal oesophagusexcised in the operation of an esophageal cancer patient; (2) longitudinally cutting open the normal oesophagus along the long axis, flattening the cut normal oesophagus, trimming the flattened normal oesophagus to remove subcutaneous connective tissues and muscles, and repeatedly flushing the trimmed normal oesophagus with a pre-cooled PBS buffer solution containing double antibodies; (3) addinga neutral protease to perform digestion; (4) peeling the epithelial layer and the basal layer; (5) cutting epithelial tissues into pieces, and adding collagenase to digest the epithelial tissue pieces; (6) carrying out digestion termination, filtration, centrifugation and washing, re-suspending the obtained material in a conditioned medium, and inoculating a culture bottle with the obtained solution; (7) placing the inoculation bottle in a 37 DEG C and 5% CO2 incubator, and performing culture; (8) carrying out cell passage and purification; and (9) observing and identifying the morphology ofcells. The method for separating and culturing the human esophageal epithelial cells is simple, stable and efficient, and provides a good experimental material for further researches on esophageal mucosal epithelium differentiation, esophageal pathophysiology, toxicology and the cancerogenesis mechanism.
Owner:JIANGYIN CHI SCI
Separating and culturing method for olfactory ensheathing cells of rats
InactiveCN107603952AEffective destinationAchieve purificationNervous system cellsTissue ProcessorDigestion
The invention provides a separating and culturing method for olfactory ensheathing cells of rats. The separating and culturing method comprises the following steps: (1) disinfecting a tissue processing instrument; (2) selecting male SD rats of 2-3 months old, killing the male SD rats after anesthesia, separating the olfactory mucosa tissue by adopting surgical scissors, placing the tissue in PBSsolution which is precooled, is sterile and contains double antibodies, and washing the tissue for a plurality of times, so that blood stains and outer membranes are removed; (3) cutting up the tissue, and carrying out digestion by adopting preheated neutral enzyme and trypsin respectively; (4) terminating the digestion, carrying out centrifugation, and discarding the supernatant; (5) inoculatingthe obtained product into an enveloped culture flask by adopting an artificial wall sticking method; (6) carrying out incubation for 1-2 h, and carrying out culturing in the environment being at 37 DEG C containing 5% CO2; (7) carrying out cell purification; (8) carrying out cell morphological observation; (9) carrying out cell subculturing; (10) carrying out cell counting and survival rate testing; and (11) carrying out immunological identification. For the separating and culturing method for olfactory ensheathing cells of rats, the operation is simple, a large number of cells are obtained, the survival rate is high, the method belong to an ideal primary separating and culturing method for the olfactory ensheathing cells of rats, and a reliable cell resource is provided for the experiment.
Owner:JIANGYIN CHI SCI
Method and equipment for pressure-reduction freshness-retaining storage of wild edible mushrooms
InactiveCN103843878APrevent immersionGuaranteed appearanceFruits/vegetable preservation by freezing/coolingProcess engineeringEdible mushroom
The invention relates to a method and equipment for pressure-reduction freshness-retaining storage of wild edible mushrooms, and specifically a storage method and equipment for achieving optimal storage conditions by controlling pressure, temperature and humidity. In order to ensure a low-pressure, high-temperature and low-temperature environment of gas in a storage room, a gas pre-treating chamber is additionally arranged on conventional pressure-reduction equipment; low-pressure, high-humidity and low-temperature needs of the storage room on the gas are achieved by utilizing a vacuum pump, an air humidifier and a refrigerating device to carry out pre-treatment on the gas entering the storage room; meanwhile, the storage room is connected with the vacuum pump and the refrigerating device, so that the device is in fresh moist gas flow with constant low pressure and low temperature all the time.
Owner:云南云菌科技(集团)有限公司
Method for separating and culturing pituitary gland cells of rat
InactiveCN107267438AEasy to makeHigh purityArtificial cell constructsCell culture supports/coatingPituitary glandPre cooling
The invention provides a method for separating and culturing pituitary gland cells of a rat. The method comprises steps as follows: (1), an 8d male SD rat is selected and subjected to anesthesia execution; (2), the anesthetized rat is fixed on a board with the ventral side facing upwards, the pituitary gland of the rat is taken out under the sterile condition, put in a pre-cooled PBS (poly(butylene succinate)) liquid and washed repeatedly, bloodstain is removed, and pituitary gland envelope is removed; (3), the pituitary gland is cut into 1 mm*1 mm fragments by ophthalmic scissors in the pre-cooled PBS liquid under the sterile condition; (4), pre-heated III type collagenase is combined with neutral enzyme for mixed digestion; (5), the digestion is stopped, filtering is performed, a filtrate is collected and centrifuged, a supernatant is abandoned, a culture medium is added for resuspension, and the operation is repeated three times; (6), a 24-hole culture plate is inoculated with cells for culture; (7), the cells cultured for 7d are subjected to trypsin digestion and then are purified by a screen again, a supernatant is abandoned after centrifugation, the cells are resuspended, and the treated culture plate is inoculated with the cells; (8), the cells are subjected to cell immunofluorescence identification. The method for separating and culturing the pituitary gland cells of the rat is simple to operate, the obtained pituitary gland cells are high in survival rate and good in activity, can be used for establishing a cell experimental model in vitro and meet the requirement of pituitary gland cell experiments.
Owner:JIANGYIN CHI SCI
Method of extracting high-quality DNA (Deoxyribonucleic Acid) of fin product
The invention relates to a method of extracting high-quality DNA (Deoxyribonucleic Acid) of a fin product and belongs to the field of molecular biology associated research. The method comprises the following steps: (1) pre-treating a fin product sample, namely softening and smashing fins in a weak base solution; (2) splitting the pre-treated fin product sample by a CTAB (Cetyltrimethyl Ammonium Bromide) splitting solution; (3) extracting the DNA by balanced phenol and chloroform-isoamylol in steps; (4) adding a high-saline solution of isopropanol doubled in volume into an aqueous phase containing the DNA to obtain a DNA precipitate; washing the DNA precipitate by an ethanol solution with the volume fraction of 75%, fully drying, and then, dissolving the DNA precipitate and storing. The method of extracting the DNA of the fin product provided by the invention can be used for conveniently and efficiently obtaining the high-quality DNA of a fin tissue so as to avoid pollution by polysaccharide, collagen and other impurities.
Owner:YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
Separation and culture method of rat Schwann cells
InactiveCN107338221AHigh purityA large amountCell dissociation methodsCulture processEnzyme digestionTissue Processor
The invention provides a method for isolating and culturing rat Schwann cells, comprising the following steps: (1) disinfecting tissue processing instruments; (2) selecting 2-3 week old male SD rats, killing them by neck dislocation, and using ophthalmic scissors Cut off the hair on the legs of rats, cut off the sciatic nerve, put the tissue into pre-cooled sterile PBS solution containing double antibodies and wash several times to remove blood stains and adventitia; (3) Mince the tissue, and use preheated type II collagen (4) Terminate the digestion, centrifuge, discard the supernatant, add culture medium and resuspend; (5) Inoculate the tissue block into a 6-well culture plate for culture by adherent method; (6) Incubate for 1-2 hours, Placed in a 37°C, 5% CO2 environment for culture; (7) Cell subculture and purification; (8) Cell morphology observation and identification. The method for separating and culturing rat Schwann cells provided by the present invention is simple and easy to operate, stable and efficient, and the obtained Schwann cells have high vigor, which can be used to establish model cells for in vitro experiments and meet the requirements of rat Schwann cell experiments .
Owner:JIANGYIN CHI SCI
Environment-friendly chopsticks and preparation method thereof
The invention provides a pair of environment-friendly chopsticks, which is prepared by uniformly mixing and drying reed haulm powder, xanthan gum solution and guar gum solution. The invention further provides a preparation method for the pair of environment-friendly chopsticks. The invention provides the pair of reed chopsticks which is brand-new and environment-friendly, the use is safe and convenient, environment pollution is reduced, and ecological balance is kept.
Owner:SHANGHAI OCEAN UNIV
Separation and culture method of human retina Muller cells
InactiveCN110387351AHigh purityHigh activityCell dissociation methodsNervous system cellsBottleDigestion
The invention provides a separation and culture method of primary human retina Muller cells. The separation and culture method comprises the following steps: (1) a normal eyeball surgically excised from a corneal donor is taken out aseptically; (2) under a dissecting microscope, retina tissue is detached aseptically; (3) mixed collagenase is added for digestion; (4) digestion is terminated, filtering, centrifuging and washing are conducted, re-suspending is conducted through a complete medium, and the complete medium is inoculated into a culture bottle; (5) the culture bottle is placed into a5% CO2 incubator at 37 DEG C to be cultured; (6) cell passage and purification are conducted; and (7) the cell morphology is observed and identified. According to the separation and culture method ofthe human retina Muller cells, operation is easy, stability and high efficiency are achieved, and a good experimental material is provided for further studying a retinopathy damage generation mechanism.
Owner:JIANGYIN CHI SCI
Preparation method of carbon quantum dots
ActiveCN110228802AEliminate pollutionMeet the requirements of the experimentNanoopticsNano-carbonDistillationFreeze-drying
The invention discloses a preparation method of carbon quantum dots and belongs to the field of carbon quantum dots processing. The preparation method comprises the following steps: using one of forestry and agricultural residues, kitchen garbage, food and domestic fungus or a mixture of several substances thereof, and waste materials at the bottom of a methyl methacrylate distillation reaction kettle as raw materials, and reacting through a hydrothermal method; then adjusting pH value to 1-5 by adding acid, or adjusting pH value to 8-9 by adding alkali or an oxidizing agent; reacting at 20-180 DEG C, and freeze-drying to obtain carbon quantum dots. The process of the method is simple, harmless and environmentally-friendly, and the environmental pollution problem of the waste materials atthe bottom of the methyl methacrylate distillation reaction kettle is also eliminated.
Owner:NORTHEAST FORESTRY UNIVERSITY
Volume force gradient loading method based on 3D (Three Dimensional) printing technology
ActiveCN108489784AEasy to operateLow costAdditive manufacturing apparatusPreparing sample for investigationThree dimensional modelApplied mechanics
The invention discloses a volume force gradient loading method based on a 3D (Three Dimensional) printing technology and belongs to the technical field of geotechnical engineering mechanics experiments. The volume force gradient loading method comprises the following steps: calculating ground stress of different structural planes in actual geotechnical engineering, shrinking a geotechnical specimen according to the proportion, simulating magnetic field intensity by numerical simulation software ANSYS, and obtaining the effect of a magnetic field by a magnetic substance in an electromagnetic field, thus generating one volume force; calculating out the quality of magnetic materials at all structural planes according to an electromagnetic principle; constructing a three-dimensional model of ato-be-prepared test piece, and inputting the three-dimensional model into a 3D printer. When the test piece is produced, one spray head prints a geotechnical material and the other spray head printsa magnetic substance; after the test piece is manufactured, the test piece is putted into the magnetic field; the magnetic substances are attracted by an electromagnet to generate one gradient volumeforce, and the aim of simulating an actual environment is achieved. According to the volume force gradient loading method disclosed by the invention, volume force loading can be manually controlled and the defect of volume loading non-uniformity of a traditional centrifuge is overcome.
Owner:BEIJING UNIV OF TECH
Human primary gastric cancer cell separation and culture method
InactiveCN108070560AHigh purityA large amountCell dissociation methodsCell culture supports/coatingTissue ProcessorDigestion
The invention provides a human primary gastric cancer cell separation and culture method. The method comprises the following steps of (1) disinfecting a tissue processing device; (2) using a pair of operating scissors for separating a gastric cancer tissue, placing the tissue into a precooled sterile PBS liquid containing double antibodies, and washing for multiple times to remove bloodstains andnecrotic tissues; (3) cutting up the tissues, and using preheated IV type collagenase and trypsin for digesting respectively; (4) terminating digestion, centrifuging, and removing a supernatant; (5) after carrying out cell counting, inoculating into a coated culture flask; (6) culturing at 37 DEG C in an environment with 5 percent of CO2; (7) purifying the cells; (8) subculturing the tissues; (9)observing cell morphology; (10) determining cell survival rate; (11) carrying out immunological identification. The invention provides the human primary gastric cancer cell separation and culture method which is simple to operate, the number of the obtained cells is large, the survival rate is high, the method is the ideal human gastric cancer cell primary separation and culture method, and a reliable cell resource is provided for experiments.
Owner:JIANGYIN CHI SCI
Adjustable symmetric multi-channel diversion erosion corrosion test system and method
PendingCN110132715AAvoid errorsSimple structureWeather/light/corrosion resistanceInvestigating abrasion/wear resistanceCooling coilCentrifugal pump
The invention discloses an adjustable symmetric multi-channel diversion erosion corrosion test system and method. The adjustable symmetric multi-channel diversion erosion corrosion test system comprises a gas bottle, a solution tank, a centrifugal pump, a scour runner and an electrochemical workstation, wherein the solution tank is internally provided with a cooling coil; a scour fluid inlet and ascour fluid outlet are formed in the scour runner; the scour runner is internally provided with a movable deflector; two sample mounting ports which are symmetric to each other relative to the centerof an axis of the deflector are formed in the runner wall of the scour runner; a gas outlet of the gas bottle is communicated with the solution tank through a connecting pipeline; the scour fluid outlet of the scour runner is connected with the solution tank through a return pipeline; an input port of the centrifugal pump is connected with the solution tank through a connecting pipeline; an output port of the centrifugal pump is connected with each of the scour fluid inlet of the scour runner and the return pipeline through connecting pipelines; and two electrodes mounted on samples in the sample mounting ports are respectively connected with corresponding electrode wires on the electrochemical workstation through the electrode leads.
Owner:CHINA UNIV OF PETROLEUM (EAST CHINA)
Science popularization device for sound wave visualization
InactiveCN107481594AGuaranteed flexibilityMeet the requirements of the experimentEducational modelsThreaded pipeElectric machinery
The invention relates to the science popularization field for standing wave tests, and more particularly, to a science popularization device for sound wave visualization. The device comprises a base, a horizontally arranged rolling cylinder, a driving motor to drive the rolling cylinder to rotate, an elastic string group, and a lifting and lowering support to drive the elastic string group to rise and fall. The elastic string group comprises a plurality of elastic strings arranged horizontally right above the rolling cylinder; and the elastic strings are perpendicular to the axis of the rolling cylinder. The lifting and lowering support comprises a first lifting and lowering frame and a second lifting and lowering frame arranged at two ends of the elastic string group. The cylindrical face of the rolling cylinder is provided with strips which are arranged alternately and whose colors are black and white. According to the invention, based on the height of an observer and through the rotation of a first rotating rod and a second rotating rod to adjust the distance from the elastic strings to the rolling cylinder as well as for the strips under the rotation of the rolling cylinder, it is possible to notice the wave fluctuations clearly. The rotation of a second threaded pipe makes the second threaded pipe generate certain displacement away from a first mounting plate with regard to a first threaded pipe so that the elastic strings are stretched to some length to ensure that each elastic string is tight and that the elasticity of the elastic strings meets the testing requirement.
Owner:ANHUI ORIGINAL KEPU SCI & TECH
Method for preparing carbon quantum dots by taking activated sludge as raw material
PendingCN112209363ASimple preparation processNo pollution in the processMaterial nanotechnologyNanoopticsActivated sludgeFreeze-drying
The invention discloses a method for preparing carbon quantum dots by taking activated sludge as a raw material, and belongs to the field of preparation of carbon quantum dots. The method comprises the following steps: by taking the activated sludge as a raw material, adding acid into the raw material to adjust the pH value to 1-5, or adding alkali and an oxidant to adjust the pH value to 8-9; andreacting at 20-180 DEG C, and carrying out freeze drying to obtain the carbon quantum dots. The activated sludge can be pretreated by a hydrothermal method; one or a mixture of several of forestry and agricultural residues, kitchen garbage, grains and edible mushrooms can also be added into the activated sludge. The method is simple in process, environmentally friendly and harmless, and meanwhilethe problem of activated sludge environmental pollution is solved.
Owner:NORTHEAST FORESTRY UNIVERSITY
Universal positioning apparatus for ultrasonic data acquisition method in laboratory and acquisition method thereof
ActiveCN100507553CHigh degree of simulationEnsure reliabilityMaterial analysis using sonic/ultrasonic/infrasonic wavesSeismic signal receiversExperimental researchMarine engineering
The invention belongs to a laboratory ultrasonic detection device, in particular to a device capable of setting the collection angle in all directions during the ultrasonic data collection process. The universal positioning device is arranged on the three-dimensional positioning device; the universal positioning device is connected with the host through a communication port; the three-dimensional positioning device includes a universal positioning device and a transducer; the universal positioning device is used to control the transducer Rotate for continuous data acquisition. The invention overcomes the energy inconsistency and arrangement limitation caused by the directivity of the transducer in the existing experimental system, improves the simulation degree of the seismic physics experiment system, expands the experimental research content, improves the productivity, and ensures the accuracy of the experimental data. Reliability and practicability meet the experimental requirements of oil and gas exploration and development. The purpose of high efficiency, quickness, flexibility, precision, reliability and strong practicability is achieved.
Owner:CHINA PETROLEUM & CHEM CORP +2
High-speed WFOV (wide field of view) CARS (coherent anti-stokes raman scattering) microscope system and method
ActiveCN102116929BMolecular structure influenceHigh imaging sensitivityMicroscopesNon-linear opticsFluorescenceCcd camera
Owner:BEIJING LUSTER LIGHTTECH
Visual horizontal annulus rock debris migration simulation method
InactiveCN112227989AGuaranteed structural strengthEasy to observeSurveyEducational modelsHorizontal wellsWell drilling
The invention discloses a visual horizontal annulus rock debris migration simulation method, and belongs to the technical field of petroleum drilling simulation experiments. The visual horizontal annulus rock debris migration simulation method is characterized by comprising the following steps of a, injecting liquid into an annulus of a simulation wellbore to simulate drilling fluid in a horizontal annulus of a horizontal well; b, regulating and controlling the rock debris injection amount of a rock debris tank through a feeding control valve, and analyzing the influence of different rock debris amounts on rock debris migration; c, adjusting the displacement of the drilling fluid, and analyzing the influence of different drilling fluid return speeds on rock debris migration; d, adjusting arotary eccentric device of a drill rod, and analyzing the influence of different eccentricities on rock debris migration; e, analyzing the influence of different rotating speeds of the drill rod on rock debris migration by adjusting the rotating speed of the drill rod; and f, analyzing the influence of the rock debris with different physical properties on the migration of the rock debris by injecting the rock debris with different physical properties. According to the visual horizontal annulus rock debris migration simulation method, the principle is reliable, operation is easy and convenient, and the migration situation of rock debris under various working conditions of the large-displacement horizontal annulus section of the horizontal well can be simulated more truly.
Owner:BC P INC CHINA NAT PETROLEUM CORP +1
Visual horizontal annulus rock debris migration simulation equipment
PendingCN112227988AGuaranteed structural strengthEasy to observeSurveyFlushingFrequency changerHorizontal wells
The invention discloses visual horizontal annulus rock debris migration simulation equipment, and belongs to the field of petroleum drilling simulation experiments. The visual horizontal annulus rockdebris migration simulation equipment comprises a drilling fluid device, and is characterized by further comprising a rock debris feeding device, a simulation wellbore device, a drilling rod rotatingeccentric device, a rock debris separator and a computer, the simulation wellbore device comprises a simulation wellbore and a fixed seat, the drilling rod rotating eccentric device comprises an eccentric device, a drilling rod connecting section, a drilling rod, a torque sensor, a motor and a second frequency converter, a first frequency converter, the second frequency converter, a liquid flow meter and the torque sensor are connected with the computer, and the simulation wellbore comprises an upper half wellbore body and a lower half wellbore body, the upper half wellbore body is a transparent outer wellbore, the lower half wellbore body is a rough simulation well wall, and a metal outer wellbore is arranged outside the simulation well wall. The simulation equipment is simpler in structure and lower in manufacturing cost, the migration situation of rock debris in the large-displacement horizontal annulus section of a horizontal well can be simulated more truly, and the requirements of experiments under various working conditions are met.
Owner:BC P INC CHINA NAT PETROLEUM CORP +1
Kit for detecting cow milk content in goat milk product and application of kit
InactiveCN104711352AMeet the requirements of the experimentThe detection method is accurateMicrobiological testing/measurementCow milkingBiotechnology
The invention discloses a kit for detecting cow milk content in a goat milk product and application of the kit, and belongs to the technical field of food safety. The kit comprises a specific primer and a probe for internal comparison genes as well as a primer and a probe for specific genes of a cow, wherein the specific primer for the internal comparison genes is designed and obtained after comparing the homologous sequences of 12SrRNA of the cow and a goat; the primer for the specific genes of the cow is designed for the partial nucleotide sequence of the 12SrRNA of the cow. The invention further provides a method for detecting the cow milk content in the goat milk product. The method comprises the following steps: extracting the DNA of a sample; utilizing the primer and probe for internal comparison as well as the primer and probe for the specific genes to carry out an FQ-PCR amplification reaction; quantitatively detecting the cow milk content in the goat milk product. The method is accurate, reliable, high in specificity, low in detection limit, high in reproducibility and suitable for rapid and quantitative detecting of the cow milk content in the goat milk product.
Owner:黑龙江省乳品工业技术开发中心
Human primary hepatocyte separation and culture method
InactiveCN108070551AHigh number of cellsLess damaging to cellsCell dissociation methodsArtificial cell constructsRemove bloodChemistry
The invention provides a human primary hepatocyte separation and culture method. The method comprises the following steps of (1) cutting a fresh human hepatocyte under an aseptic condition, sealing at4 DEG C and then carrying back to a lab; (2) placing the tissue into a precooled sterile PBS buffer liquid containing double antibodies, and washing for multiple times to remove blood and connectivetissues; (3) irrigating through a preheated PBS buffer liquid through a needle-punching method; (4) irrigating through a preheated HBSS buffer liquid through a needle-punching method; (5) irrigating through a preheated GBSS mixed enzyme liquid through a needle-punching method; (6) cutting up the tissue, and using the GBSS mixed enzyme liquid for digesting, centrifuging, and removing a supernatant;(7) using trypan-blue for dyeing a cell filter liquor to detect cell activity; (8) after carrying out cell counting, resuspending a complete medium, inoculating into a coated culture flask, and culturing at 37 DEG C in an environment with 5 percent of CO2; (9) identifying cell morphology; (10) detecting through an ELISA (Enzyme-linked Immuno Sorbent Assay) method; (11) detecting through RT-PCR (Reverse Transcription-Polymerase Chain Reaction). The invention provides the human primary hepatocyte separation and culture method which is simple to operate, the number of the obtained cells is large, the survival rate is high, the method is the ideal human primary hepatocyte separation and culture method, and a reliable cell resource is provided for experiments.
Owner:JIANGYIN CHI SCI
Separation and culture method for primary pig liver cells
InactiveCN109022348AA large amountHigh purityCell dissociation methodsVertebrate cellsDigestionCell morphology
The invention provides a separation and culture method for primary pig liver cells. The separation and culture method comprises: (1) selecting a 3-4-week-old male Chinese experimental minipig with thebody weight of 2.5-4 kg, carrying out absolute diet and water deprivation at the 12th h before surgery, and carrying out intraperitoneal injection of sodium pentobarbital and heparin; (2) anesthetizing the experimental pig, fixing on a plate, and cutting the abdominal cavity under aseptic conditions, wherein the abdomen surface is upward; (3) carrying out venous cannula, and perfusing with a pre-heated HBSS buffer liquid by using a constant flow pump; (4) perfusing with a pre-heated GBSS mixed enzyme solution by using the constant flow pump; (5) shearing the tissue, carrying out shaking digestion with a GBSS mixed enzyme solution, filtering with a sieve mesh, carrying out centrifugation, and discarding the supernatant; (6) taking the cell filtrate, and detecting the viability of cells byusing Trypan Blue staining; (7) after cell counting, re-suspending with a complete culture medium, inoculating into a coated culture flask, and culturing in a 37 DEG C 5% CO2 environment; (8) carryingout cell morphology identification; (9) carrying out ELISA detection; and (10) carrying out RT-PCR detection. According to the present invention, with the separation and culture method, the operationis simple, a large number of the cells can be obtained, and the survival rate is high; and the method is the ideal method for separating and culturing primary pig liver cells so as to provide the reliable cell resources for experiments.
Owner:JIANGYIN CHI SCI
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