Human primary hepatocyte separation and culture method
A technology of primary hepatocytes and a culture method, which is applied in the field of separation and culture of human primary hepatocytes, can solve the problems of inability to meet the needs of human hepatocytes, large volume requirements of liver tissue blocks, and inapplicability, and achieves the goal of achieving cell growth. The effect of good growth condition, low cost and easier preservation
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[0020] The terms used in the present invention, unless otherwise specified, generally have the meanings commonly understood by those skilled in the art.
[0021] The present invention will be described in detail below in conjunction with the accompanying drawings and examples, and the protection content of the present invention is not limited to the following examples.
[0022] In the following examples, various procedures and methods not described in detail are conventional methods well known in the art.
[0023] The experimental instruments and reagents used in this experiment are as follows:
[0024] A set of surgical instruments, CCK-8 cell counting box (Sigma, USA), inverted microscope (XDS-1A, Shanghai), fluorescence microscope (Leica, USA), cryogenic centrifuge (TD24B-WS, Shanghai), ultra-low temperature refrigerator ( Zhongke Meiling), pipette gun (Eppendorf, USA), electronic analytical balance (Sartorius, USA), ultra-clean bench (HJ-CJ-1D, Shanghai), CO2 cell incubat...
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