The present invention discloses a production method of a non-woven fabric for wound dressing. The production method comprises: mixing water, granular water-soluble chitosan and granular calcium alginate at a room temperature, and uniformly stirring to prepare an antibacterial finishing solution, wherein the concentration of the chitosan is 1-3%, and the concentration of the calcium alginate is 0.02-0.05%; placing a non-woven fabric into the blended antibacterial finishing solution, soaking, finishing, dewatering, and drying; and carrying out high temperature shaping on the treated non-woven fabric, and carrying out high temperature compounding on the shaped non-woven fabric and a thermoplastic polyurethane TPU film. According to the present invention, the non-woven fabric is preferably selected, is a non-woven cloth, and has characteristics of softness, air permeability, no fiber tow production, and assurance of fresh and health wound; the finishing solution is adopted to carry out the soaking treatment, such that the original softness of the non-woven fabric can not be damaged, the functions of bacterial killing, bacterial inhibiting, inflammation eliminating and hemostasis are provided for the non-woven fabric, and the wound can be easily and rapidly healed; and the thermoplastic polyurethane TPU film has a good water resistance and moisture permeability performance.