The invention provides a method for inducing adult liver stem cells for transforming to high metastatic
liver cancer cells and the stable
liver cancer cells screened by the method. The method comprises the following steps: S1: performing amplification, culture and preservation of mice adult liver stem cells; S2: employing a
gene transfection technology for transferring a lentiviral vector containing the Notch1
gene into the mice adult liver stem cells to obtain the mice adult liver stem cells for stable over-expression of the Notch1; S3: using the mice adult liver stem cells for stable over-expression of Notch1, employing a selective condition culture method for culture, performing in-vitro further differentiation, screening the
liver cancer cells having strong and stable tumorigenic capability; and S4: employing the screened liver
cancer cells, through an in-situ
liver transplantation tumor model having same species, liver
cancer is formed on liver, double-
lung metastasis tumor is spontaneously formed, so that the high metastatic liver
cancer cells can be obtained. The screened stable liver cancer cells by the method of the invention have the advantages of short
tumor formation time and good tumorigenicity, and have
lung metastasis capability.