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Allicin-entrapped cell microparticle drug delivery system as well as preparation method and application thereof

A drug-carrying system and micro-particle technology, applied in the field of medicine, can solve the problems of inability to achieve precise targeted transportation, volatile instability, poor physical and chemical properties of allicin, etc., and achieve high application value and potential, excellent therapeutic effect, significant The effect of anticancer activity

Active Publication Date: 2020-06-12
JIANGSU PROVINCE INST OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because the physical and chemical properties of allicin are generally poor, volatile and unstable, although the existing drug delivery system partially solves the above problems, it still cannot achieve precise targeted delivery to metastases and better play its anti-tumor effect. role of transfer

Method used

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  • Allicin-entrapped cell microparticle drug delivery system as well as preparation method and application thereof
  • Allicin-entrapped cell microparticle drug delivery system as well as preparation method and application thereof
  • Allicin-entrapped cell microparticle drug delivery system as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1D

[0026] Example 1 Preparation, characterization and drug loading detection of DATS-drug-containing microparticles

[0027] Take 4×10 B16BL6 cells in the logarithmic growth phase 7 cells / mL, irradiated by ultraviolet light for 1 h, added DATS with a concentration of 60 μM, incubated in the incubator for 10 h, collected the cell culture supernatant, centrifuged at 14000 g, 4 °C for 2 min, discarded the precipitate, and took The supernatant was centrifuged at 14,000 g at 4°C for 70 min to obtain DATS-containing microparticle precipitates, namely DATS-drug-containing microparticles. The prepared DATS-drug-containing microparticles were white precipitates at the bottom of the centrifuge tube.

[0028] Take 10 μL of DATS-medicine-containing microparticles, dilute it to 1 mL with pure water, and slowly inject it into the sample pool of the Malvern particle size analyzer, and measure the average particle size, polydispersity index (PDI) and potential of DATS-microparticles . The mea...

Embodiment 2

[0035] Example 2 Uptake of DATS-drug-containing cell microparticles by cells and tissue distribution in vivo

[0036] 1. Study on the uptake of DATS-microparticle drug delivery system by B16BL6 and TC-1 cells

[0037] Blank microparticle extraction: take 4×10 B16BL6 cells in the logarithmic growth phase 7 cells / mL, irradiated with ultraviolet light for 1 h, continued to incubate in the incubator for 10 h, collected the cell culture supernatant, centrifuged at 14000 g, 4 °C for 2 min, discarded the precipitate, and took the supernatant, 14000 g, 4 Centrifuge for 70 min at ℃ to obtain Blank MPs. Preparation of PKH26-DATS-microparticles: Take DATS-microparticles, dilute them to a total protein concentration of 800 ug / mL, take 0.1 mL of DATS-MPs, resuspend in 0.5 mL of DiluentC, record as A, take 2 Add μL of PKH26 to 0.5 mL of DiluentC, denoted as B, then mix A and B, incubate at 25°C for 3 min, then add an equal volume of serum to terminate the reaction, and then centrifuge the...

Embodiment 3

[0043] Example 3 Evaluation of the therapeutic effect of DATS-microparticles on the lung metastasis of mouse melanoma and the intervention of inflammatory microenvironment

[0044] C57BL6 black mice with a body weight of about 18 g were fed adaptively for 7 days and injected with 5×10 5 / mL of B16BL6 melanoma cells, after the mice were inoculated with tumor cells, the mice in each group were randomly divided into model group, blank microparticle group, DATS-microparticle and DATS bare drug group, with 5 mice in each group. Weigh the body weight of the small black mice every day, observe and record the state and death of the animals, start the administration after 23 days, intraperitoneal injection, the administration concentration is 1.8 mg / kg, and administer the administration every other day, after 14 days, the eyeballs are removed to take blood, and the cervical spine is removed The black mice were sacrificed, dissected, and the lung tissues of the mice were taken, and the ...

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Abstract

The invention relates to an allicin-entrapped cell microparticle drug delivery system as well as a preparation method and application thereof, traditional Chinese medicine garlic active ingredient organic sulfide is loaded into tumor cell microparticles, and the drug delivery microparticles have biocompatibility, higher metastatic focus organ targeting and better tumor metastasis resistance. The invention also relates to a preparation method of the drug delivery system and application of the drug delivery system in preparation of anti-tumor metastasis drugs. The organ-targeted allicin drug-containing cell microparticles obtained according to the preparation method provided by the invention have a remarkable inhibition effect on melanoma lung metastasis, Lewis lung cancer lung metastasis and other tumor metastasis, and the allicin-entrapped cell microparticle drug delivery system is a traditional Chinese medicine anti-tumor metastasis drug delivery system with biocompatibility for accurately targeting metastatic lesion organs.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a drug-carrying system of cell microparticles containing allicin, its preparation method and its application in anti-tumor lung metastasis. Background technique [0002] Tumor metastasis is an important cause of clinical death, and about 90% of malignant tumors die from distant tumor metastasis. Tumor cells infiltrate into surrounding tissues, spread to other tissues and organs of the body through the blood system and lymphatic system to form metastasis, and eventually lead to the death of the patient. The lung is the main metastatic target organ of all malignant tumors, and about 30%-54% of malignant tumors metastasize to the lung in the natural course of disease. According to the autopsy report, almost 1 / 3 of the patients who died of cancer were accompanied by lung metastasis. Existing anti-metastasis treatment strategies are mostly limited to in situ resection p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/50A61K47/46A61K31/255A61P35/04
CPCA61K9/5068A61K31/255A61P35/04
Inventor 刘玉萍陈彦瞿鼎刘聪燕
Owner JIANGSU PROVINCE INST OF TRADITIONAL CHINESE MEDICINE
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