46 results about "Osseous metastasis" patented technology

The present invention describes methods of treating cancer, cancer metastasis, and drug resistant cancers using miRNA inhibitors; for example, inhibitors of miR-409-5p. Also described are methods of using the miRNA as biomarkers; for example, to predict responsiveness to a cancer drug, to detect a disease state of cancer.

The invention provides a fluorescence labeled human triple-negative breast cancer osseous metastasis cell line. The fluorescence labeled human triple-negative breast cancer osseous metastasis cell line is prepared with a method as follows: a human triple-negative breast cancer cell MDA-MB-231 is cultured; a lentivirus carrier containing RFP (red fluorescence protein) is amplified, and an RFP labelled MDA-MB-231 cell is prepared; the RFP-MDA-MB-231 cell is transplanted to the fourth pair breast fat pad position of a nude mouse and grows into tumor; shin bone of a hind limb is taken after 4-6 weeks, bone marrow is flushed with a buffer solution and digested with collagenase, a single cell from the bone marrow is obtained, in-vitro culture amplification is performed, and red fluorescence cells are sorted with a flow cytometry; through tumor formation screening in a body of the nude mouse, the red fluorescence labelled MDA-MB-231 human triple-negative breast cancer osseous metastasis cellline is obtained. The human triple-negative breast cancer osseous metastasis cell line has the characteristics of osseous metastasis, is labelled by fluorescent protein and realizes visualization andtraceability.

The invention discloses an isotopic tracing method for monitoring cancer cell osseous metastasis. The method refers to a measurement method by taking 41Ca as a tracer agent and taking accelerator mass spectrometry (AMS) as a tracer agent and is used for monitoring the occurrence, development and resolution processes of the cancer cell osseous metastasis, and belongs to the technical field of nuclear technologies. The method disclosed by the invention comprises the following steps: (1) generating a 41Ca marked tracer agent; (2) marking an experimental nude mouse by using the 41Ca marked tracer agent; (3) collecting a urine sample of the nude mouse in the step (2), and separating the element Ca; (4) measuring the 41Ca / 40Ca isotope abundance ratio in the calcium fluoride powder obtained in the step (3); and (5) drawing a standard curve; and (6) analyzing the difference, so as to obtain the cancer cell osseous metastasis degree. According to the method disclosed by the invention, the problems that the sensitivity of a conventional monitoring means is relatively low and the early monitoring of the cancer cell osseous metastasis is difficultly realized are solved, and the method has high clinical application values and social benefits.

The invention discloses an application of miR-133b in diagnosing, forecasting and treating prostate cancer with osseous metastasis. The invention researches and finds that the miR-133b has low expression in prostate cancer and has lower expression in osseous metastasis of prostate cancer; a further research proves that the low expression of miR-133b is related to the osseous metastasis of prostate cancer and indicates shorter osseous metastasis-free survival period; besides, over-expression miR-133b is capable of in vitro restraining migration and invasion of prostate cancer and in vivo restraining osseous metastasis of prostate cancer; the miR-133b mimics also can in vitro restrain the osseous metastasis of prostate cancer; the above results prove that miR-133b can be used for preparing diagnostic reagent, forecasting reagent and osseous metastasis restraining drug for the prostate cancer with osseous metastasis.

The invention discloses an application of TSPAN1 in diagnosis, prognosis and treatment of breast cancer metastasis. The invention finds the high expression of TSPAN1 in tissues and cells of breast cancer metastasis and the high expression indicates shorter survival time without osseous metastasis; in vivo and in vitro experiments prove that the down-regulated expression of TSPAN1 genes can restrain the breast cancer metastasis and invasion so as to restrain the osseous metastasis of breast cancer; on the basis of the finding, the invention provides application of a reagent for detecting the expression level of the TSPAN1 gene in preparing a kit for detecting if breast cancer metastasis occurs and / or a prognosis kit for forecasting the breast cancer metastasis trend; a TSPAN1 inhibiting reagent is used for preparing the drug for preventing and / or treating breast cancer metastasis, and a new thought is supplied for diagnosis, prognosis and treatment of breast cancer metastasis.

A compound of formula (I), or a pharmaceutically acceptable salt, hydrate, complex or pro-drug thereof,wherein: one of R1 and R2 is H, and the other is selected from OR6, SR6, NR6R7, N3, Me, Et, CF3, SOR8 and SO2R8; orR1 and R2 are both H;one of R3 and R4 is H, and the other is selected from tert-butylmethyl, iso-propylmethyl, sec-butyl, tert-butyl, cyclopentyl and cyclohexyl; orR3 and R4 are joined together with the adjacent backbone carbon atom to form a spiro-C5-C6 cycloalkyl group;R6 and R7 are each independently selected from H, C1-8-alkyl and C3-8-cycloalkyl; orR6 and R7 are linked to form a cyclic group together with the nitrogen to which they are attached;R8 is C1-8-alkyl or C3-8-cycloalkyl;R9 is a para-substituted 6-membered monocyclic aryl or heteroaryl ring which includes up to five heteroatoms.The invention further relates to pharmaceutical compositions comprising compounds of formula (I), and the use of such compounds in the treatment of a disease selected from osteoporosis, Paget's disease, Chagas's disease, malaria, gingival diseases, hypercalaemia, metabolic bone disease, diseases involving matrix or cartilage degradation, and bone cancer disorders such as bone metastases and associated pain.

The present invention describes methods of treating cancer, cancer metastasis, and drug resistant cancers using miRNA inhibitors; for example, inhibitors of miR-409-5p, miR-409-3p, miR-154*. Also described are methods of using the miRNA as biomarkers; for example, to predict responsiveness to a cancer drug, to detect a disease state of cancer.

The invention discloses the application of an inhibitor GSK-J4 of histone demethylase to preparation of a therapeutic drug for osteosarcoma. That histone deemthylase KDM5B is associated with the treatment of the osteosarcoma is proved for the first time. GSK-J4 acts on the histone demethylase KDM5B to significantly inhibit proliferation of osteosarcoma cells, and therefore, can be used as a new breakthrough for preparing a drug for treating the osteosarcoma. Besides, the invention also discloses the ability of the GSK-J4 for inhibiting transfer of the osteosarcoma cells by targeting the KDM5Bfor the first time, and provides a novel idea for researching and developing a drug for treating lung metastasis and osseous metastasis of the osteosarcoma.

The invention discloses an antitumor combined medicine. The combined medicine comprises a diphosphonate compound serving as an active ingredient and CIK cells, wherein the diphosphonate compound is at least one of clinical medication etidronate disodium, clodronate disodium, pamidronate disodium, tiludronate disodium, alendronate sodium, neridronate sodium, olpadronate sodium, risedronate sodium, sodium ibandronate, incadronate disodium and zoledronic acid. The method for greatly improving the tumor cell killing capability of CIK cells by utilizing combination of anti-tumor-osseous-metastasis diphosphonate medicines and the CIK cells, so that the number of CIK cells for achieving the treatment effect the same as a conventional method is greatly reduced; and compared with biological treatment of single CIK cells, the combined medicine has the advantages that the relatively high safety of CIK cells can be maintained, and the treatment effect can be remarkably improved.

The invention provides an external-use drug for treating osseous metastasis cancer pain and a preparation method of the external-use drug. The external-use drug comprises scutellaria barbata, black nightshade, cantharides, rhizoma corydalis, curcuma zedoary, pericarpium citri reticulatae viride, long-noded pit vipers, centipedes, scorpions, rhizoma sparganii, borneol and asarum. The contents of the scutellaria barbata, the black nightshade, the cantharides, the rhizoma sparganii and the curcuma zedoary are the same and are highest. The contents of the pericarpium citri reticulatae viride and the rhizoma sparganii are the same. The contents of the borneol and the asarum are the same and are lowest. The external-use drug has the advantages that multiple proper amounts of Chinese herbs are matched mutually and used jointly for treatment coordination, and accordingly, the curative effect of obviously relieving the osseous metastasis cancer pain can be achieved; the preparation method is simple, the external-use drug can be made into liquid, the liquid can be also made into a plaster for external application, the dosage form of the drug can be flexible and changeable, and accordingly, needs of different patients can be met.

The invention belongs to the field of biotechnology and microorganism animal cell line and particularly relates to a human big cell lung cancer cell strain with high osseous metastasis potency and a purpose of the human big cell lung cancer cell strain with high osseous metastasis potency. A human big cell lung cancer parental generation cell NCI-H460 is utilized as a source cell for screening an osseous metastasis cell, the human big cell lung cancer cell line H460BM with high osseous metastasis potency is screened, cultivated and built by screening six times inside and outside an immunodeficient mice. A preservation number is CGMCC No. 5406. The cell line can be used as an ideal cell model of directional osseous metastasis research of human lung cancer and for transformation mechanism research and anti-metastasis drug screening of human cancer.

The invention discloses a metastatic bone tumor special disease database construction method and system. The method comprises the following steps: respectively establishing a first set, a second set and a third set; retrieving the first part of the medical record according to a preset field; retrieving a second part, a third part and a fourth part of the medical record in the second set according to a preset field; traversing the full-text texts of the medical records in the first set, and intercepting evidence fragments; and judging whether the medical record is positive or not according to the evidence fragment, and if so, storing the medical record into a positive database. By the adoption of the metastatic bone tumor special disease database construction method and system, clinical data of patients with metastatic bone tumors can be automatically collected in a full-period and full-dimension mode, and structured processing and storage of the clinical data of the patients with metastatic bone tumors are achieved.

The invention provides a traditional Chinese medicinal composition with an effect of relieving ostealgia of a tumor patient, and an application of the traditional Chinese medicinal composition in pain-easing. The traditional Chinese medicinal composition with the effect of relieving ostealgia of the tumor patient is prepared from the following raw materials in parts by weight: 15-30 parts of rhizoma drynariae, 2-4 parts of processed scolopendra, 15-30 parts of herba epimedii, 3-18 parts of pyritum, 3-18 parts of processed radix aconite, and 3-18 parts of processed radix aconiti kusnezoffii. The traditional Chinese medicinal composition with the effect of relieving ostealgia of the tumor patient has an effective pain-easing effect in treatment of osseous metastasis of cancer, a synergy curative effect on osseous metastasis, and effects of reliving ostealgia, remarkably lowering the side effect of diphosphonate and greatly improving quality of life and immunity of the patient.

Provided is an anticancer compound including a cleavable linker specifically activated in a tumor microenvironment, and use thereof. The anticancer compound is represented by the following formula, wherein, R1 is a normal functional group or a protection group; R2 is Ala, Thr, Val or Ile; R3 is Ala, Val or Asn; R4 is a drug group linked via a hydroxyl group or an amino group; and the general formula of the drug is R4H. The anticancer compound is only activated at a local portion of a tumor, thus avoiding the defect of immune system damage of a traditional chemotherapeutic drug, and promoting tumor immunization by removing a tumor immunosuppression cell. The anticancer compound or pharmaceutical composition thereof is jointly used with immunotherapy, thus improving the effect of treating the tumor, and effectively inhibiting tumor metastasis and osseous metastasis.

The invention discloses an application of miR-339-5p in retraining prostate cancer with osseous metastasis and in a TGF-beta signal channel. The invention researches and finds that the miR-339-5p has low expression in prostate cancer with osseous metastasis and a further research proves that the miR-339-5p is capable of restraining the activity of the TGF-beta signal channel through SMAD2, SMAD3 and TGFBRI in a targeting TGF-beta signal channel so as to restrain the migration and invasion of the cells of prostate cancer with osseous metastasis, so that the miR-339-5p is expected to be used for preparing a diagnostic reagent for prostate cancer with osseous metastasis, a drug for restraining osseous metastasis, a TGF-beta / smad inhibitor, an SMAD2 inhibitor, an SMAD3 inhibitor and a TGFBRI inhibitor.