Bifunctional stent with anti-tumor and tissue defect repair effects and preparation method

A repairing, dual-function technology, applied in the field of biomedical materials, can solve the problems of difficult complete resection of cancer cells, easy recurrence, and difficulty in self-regeneration of bone defect sites, avoiding secondary surgery and tumor recurrence, and promoting osteoblasts. Differentiation and the effect of promoting tumor ablation

Active Publication Date: 2021-06-29
SOUTHERN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the local tumor ablation of bone metastases is mainly surgical resection, but it is difficult to completely remove the cancer cells, easy to relapse and inevitably accompanied by bone defects
In addition, the bone tissue of patients with bone metastases is often eroded by metastatic tumor cells, and osteoblast differentiation disorder makes it difficult for bone defects to regenerate themselves, which is a major clinical problem that has not yet been resolved
The clinical treatment of bone regeneration mainly includes bone grafts and material-guided tissue regeneration, but there are usually risks of secondary surgery and accompanying immune inflammation

Method used

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  • Bifunctional stent with anti-tumor and tissue defect repair effects and preparation method
  • Bifunctional stent with anti-tumor and tissue defect repair effects and preparation method
  • Bifunctional stent with anti-tumor and tissue defect repair effects and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A bifunctional scaffold FeMg-SC is prepared by the following method:

[0031] (1) Take 6.2mg FeCl 3 ·6H 2 O, 142.5mg MgCl 2 and 45mg of dopamine hydrochloride, stirred in 130mL of pure water for 1h, quickly injected 20mL of Tris solution (22.5mg / ml), continued to stir for 3h, centrifuged at 10000rpm for 20min, retained the precipitate, washed 3 times with water, and obtained FeMg-NPs (complexed polydopamine nanoparticles of iron and magnesium ions).

[0032] (2) Dissolve 3 g of polylactic-glycolic acid in 10 mL of dichloroethane to obtain a PLAG / DCM solution.

[0033] (3) Add 3 g of β-TCP into the PLAG / DCM solution, and ultrasonically treat it in an ice bath for 30 minutes to form a uniformly dispersed β-TCP / PLAG / DCM composite solution.

[0034] (4) Take 0.3mg FeMg-NPs, 50μL Tween 20 and 1mL ultrapure water, add it to the β-TCP / PLAG / DCM composite solution, and ultrasonicate for 5min to form a uniform FeMg-PDA / DI water / TCP / PLGA / DCM Compound emulsified paste.

[003...

experiment example 1

[0058] Compressive strength and modulus of elasticity tests.

[0059] The brackets of Example 1 and Comparative Examples 1-3 were subjected to compression tests, and the mechanical strength of different brackets was tested using a universal material testing machine, and the corresponding compressive strength and elastic modulus were calculated according to the stress-strain curve obtained from the test.

[0060] Test results such as image 3 shown, from image 3 It can be seen that the compressive strength and elastic modulus of the scaffold Blank-SC, Mg-SC, Fe-SC and FeMg-SC are equivalent, the compressive strength is about 2.4±0.4MPa, and the elastic modulus is about 12.1±1.3MPa .

experiment example 2

[0062] In vivo tumor ablation experiments.

[0063] The subcutaneous model of 4T1 mouse breast cancer was established by using the existing conventional method. After the model was successfully established, the scaffolds of Example 1 and Comparative Examples 1-3 were orthotopically implanted into the tumor respectively. Continuously illuminated for 3 days in the same environment, the light wavelength is 808nm, and the power density is 1W / cm 2 , each light treatment time is 4min, and then continue to raise normally until the 14th day. The tumor volume and mouse weight were measured regularly every day to evaluate the anti-tumor effect of the printed scaffold. At the same time, set up two control groups without the above-mentioned light, that is, inject or implant PBS and the scaffold of Example 1 into the tumor respectively, and raise them normally for 14 days under the same environmental conditions, and measure the weight and volume of the tumor every day size.

[0064] The...

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Abstract

The invention provides a bifunctional stent with anti-tumor and tissue defect repair effects and a preparation method thereof, and relates to the technical field of biomedical materials. The bifunctional stent is prepared by the following steps that nano-particles are prepared, specifically, iron salt, magnesium salt, dopamine and / or dopamine hydrochloride are uniformly mixed to obtain nano-particles of polydopamine complexed iron ions and magnesium ions; emulsion paste is prepared, specifically, polylactic acid-glycolic acid, an organic solvent, tricalcium phosphate, a surfactant and the nanoparticles are uniformly mixed to obtain composite emulsion paste; and printing and forming are carried out, specifically, the composite emulsion paste is used as printing ink, and printing is carried out to form a stent. The bifunctional stent with the anti-tumor and tissue defect repair effects has the effects of killing tumor cells and promoting osteoblast differentiation in cooperation with CDT / PTT, and can be applied to resisting postoperative recurrence of bone metastatic tumors and tissue defects.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a dual-functional scaffold with anti-tumor and tissue defect repair functions and a preparation method. Background technique [0002] Bone is the most common metastatic site of many advanced cancers, such as lung, breast, and prostate cancers. When cancer cells metastasize to bone tissue, they interact with osteoclasts and osteoblasts in the bone microenvironment, resulting in an imbalance between bone resorption and bone formation, eventually causing bone-related events such as pathological fractures and pain, which seriously affect patients quality of life. [0003] At present, the local tumor ablation of bone metastases is mainly surgical resection, but it is difficult to completely remove the cancer cells, easy to relapse and inevitably accompanied by bone defects. In addition, the bone tissue of patients with bone metastases is often eroded by metastatic tumor...

Claims

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Application Information

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IPC IPC(8): A61L27/46A61L27/18A61L27/30A61L27/54A61L27/50
CPCA61L27/18A61L27/306A61L27/54A61L27/46A61L27/50A61L2300/102A61L2300/606A61L2300/416A61L2300/216A61L2300/412A61L2430/02C08L67/04
Inventor 于梦林慧敏王翀李翔史善伟李政诗
Owner SOUTHERN MEDICAL UNIVERSITY
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