Measurement method for regulating osteoclast activation mechanism in osseous metastasis

Abstract

The invention belongs to the technical field of osteoclast activation mechanism measurement, and discloses a measurement method for regulating an osteoclast activation mechanism in osseous metastasis.The method includes the steps of collecting data information of the lungs by a lung detection instrument; regulating osteoclast activation in osseous metastasis by RANK-RANKL; extracting myelomoncytes and differentiating the myelomoncytes to osteoclasts; judging whether a patient suffers from osseous metastasis or not by screening. The method can realize identification of osseous metastasis and healthy osseous metastasis bone tissue samples directly without sample pretreatment or normal temperature and pressure conditions, and has the advantages of simple operation, high precision and the like; myelomoncytes and mesenchymal stem cells are separated out at the same time, and primary myelomoncytes obtained by separation and purification can be successfully changed into osteoclasts by induced differentiation; meanwhile, data obtained after pretreatment and feature extraction is subjected to classification training by screening, and whether the patient suffers from osseous metastasis or not can be more conveniently and quickly judged.

Description

technical field [0001] The invention belongs to the technical field of measuring the activation mechanism of osteoclasts, in particular to a measuring method for regulating the activation mechanism of osteoclasts in bone metastasis. Background technique [0002] Bone metastasis is one of the malignant tumors with the fastest increasing morbidity and mortality and the greatest threat to the health and life of the population. Bone metastasis is one of the terminal events in the advanced stage of bone metastasis, which can cause pathological fractures, intractable bone pain, osteoporosis, hypercalcemia, spinal cord compression and other nerve compression syndromes and other adverse events. Bone metastases are mainly osteolytic bone metastases, which are characterized by dissolution, destruction and resorption of bone tissue. The activation of osteoclasts is the key link in the process of bone destruction and resorption. Tumor cells can activate the RANK / RANKL / OPG pathway to st...

AI Technical Summary

Problems solved by technology

[0004] (1) The current understanding of the upstream pathway of RANK-RANKL in bone metastasis is limited, and the analysis speed of information in traditional examinations is slow, and the classification is not clear, which has a certain impact on the diagnosis of the disease

[0005] (2) Osteoclast is a kind of terminal cell, and there is no mature osteoclast strain, so it is difficult to obtain; the traditional control system needs to traverse all node parameters when assigning a task, the number of nodes is large, and the control is complicated, which is difficult for subsequent Delays in the determination of the measurement not only cause a waste of time, but also cause large errors in the measurement results

[0006] (3) The chromaticity and brightness displayed by the traditional display system are low, the accuracy of the primary color coordinates is low, the display effect is poor, and clear observation cannot be performed

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