The invention discloses a kit for detecting 15
gene mutation sites related to rectal
cancer chemoradiotherapy sensitivity and use thereof. The detection kit is based on a high-
throughput DNA sequencing technology and used for detecting
DNA mutation sites of 15 genes related to the rectal
cancer chemoradiotherapy sensitivity, and comprises
specific primers for detecting the
gene mutation sites. The upstream primers of the
specific primers have
nucleotide sequences shown as SEQ ID NO.1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27 and 29 and the downstream primers of the
specific primers have
nucleotide sequences shown as SEQ ID NO.2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28 and 30. The kit has good stability for detecting
gene DNA mutation sites of a
tumor tissue specimen, high clinical
operability and a simple and convenient detection process. The kit can be used for distinguishing a patient with local advanced rectal
cancer sensitive to neoadjuvant radiotherapy and
chemotherapy, such that the patient with local advanced rectal cancer is remarkably benefited in the neoadjuvant radiotherapy and
chemotherapy.