Human big cell lung cancer cell strain with high osseous metastasis potency

A technology for human large cell lung cancer and bone metastasis, applied in the fields of biotechnology and microbial animal cell lines, can solve the problems of high metastasis, high mortality rate of lung cancer, and unclear molecular mechanism of lung cancer metastasis.

Inactive Publication Date: 2013-06-05
SHANGHAI SIXTH PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Benefits of technology

This patented technology allows researchers from different laboratories to create small tumors called primary lymphoid tissue (PLT) which contains many types of blood vessels made up mostly of tiny tubes filled with fluid containing proteins like albumin or other substances found inside them. These tumor vasculature plays important roles during normal physiological processes such as circulation, oxygen transport, nutrients absorption, signal transduction pathways, muscle contractions, and even brain function. PCT describes how these rare diseases affects millions worldwide each year causing deteriorating healthcare costs due to pain associated therewith.

Problems solved by technology

This patented technical problem addressed in this patents relates to identifying which parts or tissues within an individual's body may contain certain types of substances called metabolites during their growth process. These compounds can help predict how well they will grow next without causing harm if left untreated.

Method used

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  • Human big cell lung cancer cell strain with high osseous metastasis potency
  • Human big cell lung cancer cell strain with high osseous metastasis potency
  • Human big cell lung cancer cell strain with high osseous metastasis potency

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Human large cell lung cancer cell line (NCI-H460, purchased from Shanghai Institute of Cellular Science, Chinese Academy of Sciences) cells were used as parental cells to plant in the left ventricle of nude mice to cause bone metastasis. 99m Bone metastases were detected by Tc-MDP micro pinhole bone scintigraphy; bone metastases were taken out and cultured in vitro, expanded and planted in the left ventricle of nude mice, and screened in vivo and in vitro by immunodeficient mice (BALB / c-nu-nu) Six The second time, the human large cell lung cancer cell line H460BM with bone metastases was successfully screened and cultured, which was deposited on October 28, 2011, with the deposit number: CGMCC No.5406, and the classification name: human large cell lung cancer cell line with bone metastases NCI-H460BM.

[0022] The culture medium is RPMI1640 or DMEM medium containing 10% calf or fetal bovine serum, pH=7, culture temperature is 37°C, 5% CO 2 .

[0023] The cells showed a...

Embodiment 2

[0025] One million H460BM cells were implanted in the left ventricle of immunodeficient mice. After two weeks, the weight of the mice dropped sharply, and dropped to about 15 grams in the third week. In the fourth week, the weight dropped to 12-13 grams. Bone scintigraphy (eg, Image 6 micro SPECT and micro pinhole bone scintigraphy), the results showed obvious bone metastasis, but the X-ray was always negative; pathological results confirmed that bone metastasis reached 100%, including spine, humerus, and mandible metastasis 100%; scapula, rib metastasis 75%, confirming that the cell H460BM is a lung cancer cell line with high potential for bone metastasis, and can be used as a good cell model for bone metastasis of human large cell lung cancer.

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Abstract

The invention belongs to the field of biotechnology and microorganism animal cell line and particularly relates to a human big cell lung cancer cell strain with high osseous metastasis potency and a purpose of the human big cell lung cancer cell strain with high osseous metastasis potency. A human big cell lung cancer parental generation cell NCI-H460 is utilized as a source cell for screening an osseous metastasis cell, the human big cell lung cancer cell line H460BM with high osseous metastasis potency is screened, cultivated and built by screening six times inside and outside an immunodeficient mice. A preservation number is CGMCC No. 5406. The cell line can be used as an ideal cell model of directional osseous metastasis research of human lung cancer and for transformation mechanism research and anti-metastasis drug screening of human cancer.

Description

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Claims

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Application Information

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Owner SHANGHAI SIXTH PEOPLES HOSPITAL
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