Application of miR-582 in preparing prostatic cancer osseous metastasis diagnosis and prognosis kit and medicine

A technology of mir-582 and pri-mir-582, applied in the field of biomedicine, can solve the problems of incurable prostate cancer and low survival rate of patients

Active Publication Date: 2018-06-12
THE SECOND AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The main cause of death of prostate cancer is distant metastasis of tumor, especially bone metastasis. Although primary prostate cancer can be ef...

Method used

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  • Application of miR-582 in preparing prostatic cancer osseous metastasis diagnosis and prognosis kit and medicine
  • Application of miR-582 in preparing prostatic cancer osseous metastasis diagnosis and prognosis kit and medicine
  • Application of miR-582 in preparing prostatic cancer osseous metastasis diagnosis and prognosis kit and medicine

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Effect test

experiment example 1

[0044] Experimental Example 1. Low expression of miR-582-3p and miR-582-5p in bone metastatic prostate cancer tissue

[0045] In the early stage, the TCGA database was used to analyze the miRNA sequences in prostate cancer patients, and the results were as follows: figure 1 As shown, compared with normal tissues, miR-582-3p and miR-582-5p were highly expressed in prostate cancer tissues (both Pfigure 1 A and figure 1 B).

[0046] Further research unexpectedly found that among 149 prostate cancer tissues, miR-582-3p and miR-582-5p were significantly down-regulated in 63 prostate cancer tissues with bone metastases compared to 94 prostate cancer tissues without bone metastasis (e.g. figure 1 C and figure 1 D), which is about 0.5-0.6 times that of prostate cancer tissue without bone metastasis.

[0047] According to the median expression of miR-582-3p and miR-582-5p in prostate cancer tissues, miR-582-3p and miR-582-5p were stratified into high expression (H) and low expressio...

experiment example 2

[0048] Experimental Example 2. Low expression of miR-582-3p and miR-582-5p in prostate cancer cell lines with bone metastases

[0049] Taking prostate cancer cell lines as the research object, normal human prostate epithelial cells RWPE-1 and human prostate cancer cell lines DU145, LNCaP, 22Rv1, PC-3, VCaP, C4-2B were cultured, and miR-582-3p and miR The expression level of -582-5p, the results are as follows figure 2 As shown, compared with the normal human prostate epithelial cell line RWPE-1, the expression of miR-582-3p and miR-582-5p in bone metastatic prostate cancer cell lines PC-3, C4-2B, and VCaP were all low. demonstrated low expression of miR-582-3p and miR-582-5p in bone-metastatic prostate cancer cell lines.

experiment example 3

[0050] Experimental example 3, the low expression of miR-582-3p and miR-582-5p predicts the low bone metastasis-free survival rate of prostate cancer patients

[0051] Combined with the clinicopathological data of prostate cancer tissues, miR-582-3p and miR-582-5p were stratified into high expression levels ( H) and low expression level (L), Kaplan-Meier survival analysis was performed on cases in each stratification, the results are as follows image 3 showed that the expression levels of miR-582-3p and miR-582-5p were not related to the overall survival rate of prostate cancer patients, however, the low expression of miR-582-3p and miR-582-5p both predicted lower Bone metastasis-free survival.

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Abstract

The invention discloses application of miR-582 in preparing a prostatic cancer osseous metastasis diagnosis and prognosis kit and medicine. It is unexpectedly found that miR-582-3p and miR-582-5p havea low expression in prostatic cancer tissue of osseous metastasis, and the low expression of miR-582-3p or miR-582-5p predicts a lower bone-free metastasis survival rate; besides, an in-vivo experiment and an in-vitro experiment both prove that the overexpression of pri-miR-583 can up-regulate miR-582-3p and miR-582-5p, and through a TGF-beta signal access, prostatic cancer osseous metastasis andmigration and invasion of prostatic cancer osseous metastasis cell lines are inhibited. Thus, based on the findings, miR-582 can be applied to preparing the prostatic cancer osseous metastasis diagnosis and prognosis kit, and the medicine inhibiting prostatic cancer osseous metastasis is expected to be developed.

Description

technical field [0001] The invention belongs to the field of biomedicine, and more specifically relates to the application of miR-582 in the preparation of diagnostic and prognostic kits and medicines for bone metastasis of prostate cancer. Background technique [0002] Worldwide, prostate cancer (Prostate Cancer, PCa) is one of the most common malignant tumors in men and the second leading cause of cancer death, second only to lung cancer. The main cause of death of prostate cancer is distant metastasis of tumor, especially bone metastasis. Although primary prostate cancer can be effectively controlled by surgery, hormone therapy, radiotherapy and other means, prostate cancer with bone metastasis is still incurable. The rate is extremely low. Therefore, the early diagnosis of bone metastases is very critical for the prognosis and diagnosis and treatment of prostate cancer. At the same time, the development of therapeutic drugs for bone metastasis of prostate cancer is also...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886A61K45/00A61P35/04
CPCA61K45/00C12Q1/6886C12Q2600/118C12Q2600/158
Inventor 黄帅唐欲博郭远清瓦庆德廖壮文
Owner THE SECOND AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIV
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