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Albumin-loaded manganese dioxide and perflubron nanoparticles as well as preparation method and application thereof

A technology of perfluorooctane bromide and manganese dioxide, applied in the directions of nanotechnology, nanotechnology, nanomedicine, etc., can solve the problems of unreported synergy

Pending Publication Date: 2020-10-27
AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

MnO 2 Synergy of nanoplatforms in HIFU therapy has not been reported

Method used

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  • Albumin-loaded manganese dioxide and perflubron nanoparticles as well as preparation method and application thereof
  • Albumin-loaded manganese dioxide and perflubron nanoparticles as well as preparation method and application thereof
  • Albumin-loaded manganese dioxide and perflubron nanoparticles as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1 Albumin-loaded manganese dioxide and perfluorooctyl bromide nanoparticles (MnO 2 -Preparation and characterization of PFOB@BSA)

[0040] An albumin-loaded manganese dioxide and perfluorooctyl bromide nanoparticles and a preparation method thereof, comprising the steps of:

[0041] A. Weigh 120mg of BSA and dissolve it in 12mL of ultrapure water. Under magnetic stirring at room temperature, slowly add 0.8 mL of 100 mM MnCl to the above solution 2 · 4H2O, after stirring for 5min, adjust the pH of the solution to 11 with 1.0M NaOH.

[0042] B. After reacting under vigorous stirring at room temperature for 2 hours, the nanoparticle suspension was further filtered through a filter membrane (Millipore, pore size: 220 nm). Afterwards, the product was dialyzed with deionized water for 48 hours, and the molecular weight cut-off of the dialysis bag was 30 kDa, so as to remove excess unreacted precursors.

[0043] C, collect the solution in the dialysis bag, cool t...

Embodiment 2

[0046] Example 2 In vitro toxicity to human umbilical vein endothelial cells HUVEC, human malignant glioblastoma cells U87MG and mouse breast cancer cells 4T1, and glutathione consumption in 4T1 cells

[0047] An application of albumin-loaded manganese dioxide and perfluorooctyl bromide nanoparticles. The logarithmic growth phase of HUVEC, U87MG and 4T1 cells was digested with trypsin to make a single cell suspension, and the cell density was adjusted to 5× 10 3 / well, 200 μL per well was inoculated in a 96-well cell culture dish, and three replicate wells were set for each nanometer concentration, at 37°C, 5% CO 2 Incubate in an incubator for 24 hours. After 24 hours, take out the 96-well plate, suck off the original medium, add 100 μL of nano-solutions with different concentrations of 0, 6.25, 12.5, 25, 50, and 100 μg / ml to each well, at 37°C, 5% CO 2Continue to incubate for 24 hours in the incubator. Aspirate the medium containing nanomaterials, wash with PBS, add 10 μ...

Embodiment 3

[0049] Example 3 Expression of immunogenic cell death biomarkers in 4T1 cells treated with albumin-loaded manganese dioxide and perfluorooctyl bromide nanoparticles and high-intensity focused ultrasound HIFU

[0050] An application of albumin-loaded manganese dioxide and perfluorooctyl bromide nanoparticles, the 4T1 cells in the logarithmic growth phase were digested with trypsin to make a single cell suspension, and the appropriate cell concentration (1×10 4 ), inoculate confocal culture dishes at 37°C, 5% CO 2 Cultivate in the incubator for 24h. After 24 hours, add manganese containing 25 μg / ml MnO diluted in medium to the 4T1 cell dish 2 - PFOB@BSA nanoparticles. After the nanoparticle incubation time reached the preset time (0h, 2h, 4h, 6h), the medium containing the nanoparticles was removed. Wash with preheated PBS, fix with 1ml of 4% paraformaldehyde for 5min, and wash with PBS. Add the immunostaining blocking solution to the confocal dish, and put it in the refri...

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Abstract

The invention discloses albumin-loaded manganese dioxide and perflubron nanoparticles as well as a preparation method and application thereof, and relates to the technical field of biology, and the albumin-loaded manganese dioxide and perflubron nanoparticles are prepared from bovine serum albumin-loaded manganese dioxide and perflubron. The invention further provides a preparation method of the albumin-loaded manganese dioxide and perflubron nanoparticle and application of the albumin-loaded manganese dioxide and perflubron nanoparticle in tumor bimodal imaging and enhancement of high-intensity focused ultrasound tumor immunogenic death. The invention has the advantages that the albumin-loaded manganese dioxide and perflubron nanoparticles obtained by the preparation method have the characteristics of enhancing high-intensity focused ultrasound immune death and biocompatibility and being high in active targeting enrichment amount in tumors, and tumor diagnosis and real-time monitoringcan be realized through CT and MRI bimodal imaging; by enhancing high-intensity focused ultrasound tumor immunogenic death, tumor growth and lung metastasis can be inhibited in vivo, and the survivalrate of animals can be increased.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to albumin-loaded manganese dioxide and perfluorooctyl bromide nanoparticles, a preparation method and application. Background technique [0002] The tumor microenvironment (Tumor Microenvironment, TME) is composed of tumor cells, peripheral blood vessels, extracellular matrix, stromal cells, fibroblasts, immune cells, pericytes, and adipocytes. Immune cells such as macrophages, lymphocytes, natural killer (NK) cells, and dendritic cells (DCs) infiltrate early on around tumor cells, helping to suppress tumor growth. However, antitumor immune responses generated by these cells are suppressed by immunosuppressive cells, including myeloid-derived suppressor cells (MDSC) regulatory T cells (Tregs) and type 2 polarized Macrophages (M2). TME has a strong immunosuppressive effect. Tumor cells evade immune cell monitoring and killing in multiple immune mechanisms. For example, dev...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/51A61K33/32A61K47/42A61K41/00A61K49/00A61K49/04A61K49/18A61P35/00A61P35/04B82Y5/00B82Y20/00B82Y25/00B82Y30/00B82Y40/00
CPCA61K33/32A61K9/5169A61K49/0002A61K41/0033A61P35/00A61P35/04A61K49/1818A61K49/04B82Y5/00B82Y20/00B82Y25/00B82Y30/00B82Y40/00
Inventor 尹波蒯新平刘莉庞志清耿道颖叶晓丹王胜裕
Owner AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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