47 results about "Glutamate oxaloacetate transaminase" patented technology

The invention discloses a system for estimating the state of a critically ill patient in an obstetrical department, which comprises an input end, an output end and a data processing module. Firstly, measured heart rate, blood pressure, body temperature, breathing rate, pH, blood HCO-3 concentration, oxygen partial pressure, alveolar arterial blood oxygen tension difference, potassium ion concentration, hematokrit, white cell count, platelet count, fibrinogen, glutamic-oxaloacetic transaminase, albumin, bilirubin, creatinine, blood glucose concentration and nervous system grading data are input the system through the input end; the data processing module evaluates a death risk factor; and at last, the output end directly reflects the result of the patient and the expert suggestion. The invention can not only accurately evaluate the critical degree of the state of the patient and predict the death risk, but also can provide quantitative indexes for changing to a three-class hospital, and is beneficial to improve the treatment level of the critical state of the obstetrical department and lower the death rate of pregnant and lying-in women and perinatal infants.

The invention relates to the biomedical field, and particularly relates to application of dihydromyricetin in preparation of a liver treatment and regeneration promotion medicine. Dihydromyricetin shows relatively high oxidation resistance and characteristic on promoting cell growth as a flavonoid compound. The test shows that dihydromyricetin can effectively relieve liver tissue necrosis caused by acute hepatic failure and can promote hepatocyte proliferation, so as to obviously raise the survival rate of a model mouse suffering from acute hepatic failure; dihydromyricetin also can obviously improve the activity of superoxide dismutase in tissue and serum, increase the content of serum protein, and lower down the levels of glutamic oxalacetic transaminase, glutamic-pyruvic transaminase and tumor necrosis factor alpha.

The invention relates to a model and a system for predicting severe new coronal pneumonia as well as an establishment method and a prediction method of the model and the system, and the prediction model is a progression risk score calculation model obtained according to lung diseases, age, immune globulin M (IgM), CD16 + / CD56 + NK cells and aspartate transaminase (AST) of a patient with new coronal pneumonia so as to predict the risk of progressing from mild / ordinary type to severe new coronal pneumonia. According to the invention, light / ordinary patients with high development risk can be identified, and the diagnosis and treatment success rate of critical patients can be improved.

The invention discloses an application of coptisine or coptisine salts in the preparation of medicines for preventing and treating fatty liver damage. The pharmacological research proves that the coptisine has the effects of lowering lipid, improving lipid metabolism and alleviating the hepatic lesion caused by hepatic tissue fat accumulation and is capable of inhibiting hepatocyte triglyceride accumulation; for experimental hyperlipemia symptoms, the coptisine has the effects of obviously reducing triglyceride, cholesterol and LDL-C (low-density lipoprotein cholesterol) in blood and reducing the content of triglyceride in hepatic tissues; for fatty liver caused by the overuse of cortisol, the coptisine is capable of reducing the accumulation of triglyceride in the liver, the rise of activity of glutamic-pyruvic transaminase (GPT) and glutamic oxalacetic transaminase (GOT) and alleviating the hepatic lesion and has an excellent fatty liver prevention effect; part indexes of the coptisine are obviously superior to those of lipid reducing medicines, namely simvastatin and fenofibrate and liver protecting medicines, namely tiopronin and glucurolactone; the coptisine can be applied to clinical fatty liver diseases.

The invention relates to a feed additive capable of restoring a drug-induced liver injury of a grass carp. The feed additive is prepared from by glutathione, vitamin C, vitamin E, organic selenium, Chinese wolfberry fruits, astragalus membranaceus, soybean protein powder and wheat middling; the ingredients are reasonably matched in a certain proportion for producing out the feed additive, when the feed additive is added to grass carp feed, glutamic oxalacetic transaminase, glutamic-pyruvic transaminase and malonaldehyde which rise to a high level in the body of the grass carp can be adjusted to the normal level, meanwhile, a good restoration effect is achieved on liver tissue pathological changes caused by drug-induced elements, and the feed additive is simple in preparation method.

The invention discloses a preparation method and an application of Thais clavigera Kuster polysaccharide. The method comprises the following steps: taking fresh Thais clavigera Kuster as an initial material, boiling with water, removing shells, taking meat, carrying out vacuum freeze drying treatment, and carrying out alkaline water extraction, ethanol precipitation, protease enzymolysis, macroporous resin decolorization and ultrafiltration to obtain the refined polysaccharide component of which proteins and pigments are removed and the molecular weight is greater than 10 kD. The Thais clavigera Kuster polysaccharide prepared in the invention has remarkable anti-hepatitis B activity, can remarkably reduce the content of glutamic-pyruvic transaminase (ALT) and glutamic oxalacetic transaminase (AST) in serum, and plays a great role in protecting the liver.

The invention relates to a red-increasing feed additive for parrot fish. The additive comprises capsanthin and vitamin E in a weight ratio of (7-15):(3-10). According to the invention, capsanthin and vitamin E which are adopted as raw materials are mixed into common feed in the ratio, and then the feed is conventionally used for feeding. Experiments prove that the surface of the parrot fish is obviously colored after the use of the additive, and the coloring effect is far greater than that of a control group; activities of glutamic-pyruvic transaminase and glutamic oxalacetic transaminase are not influenced so that no side influences occur to liver-pancreas of the parrot fish; moreover, the weight increment of the parrot fish and the activities of antioxidant indexes in the liver-pancreas are obviously influenced; in conclusion, the additive can effectively increase the color of the surface of the parrot fish and promote the growth of the parrot fish and has good effect in the increment of the antioxidant ability of the parrot fish and the accumulation of carotenoid.

The invention belongs to the technical field of aquaculture, and particularly relates to a low-protein feed for improving growth performance of litopenaeus vannamei and application thereof. The low-protein feed comprise nucleotide, and is used for improving the digestion and absorption capacity of the litopenaeus vannamei to nutrient substances in feed, improving growth performance of the litopenaeus vannamei, improving the body composition and serum biochemical indexes of the litopenaeus vannamei and improving immunity of the litopenaeus vannamei. The nucleotide comprises 5'-cytidylate, 5'-adenylate, disodium 5'-inosinate, disodium 5'-uridylate, disodium 5'-guanylate and disodium 5'-thymidylate. The low-protein feed of the present invention is mainly used for improving crude fat content,serum glutamic oxalacetic transaminase and glutamic-pyruvic transaminase, hepatopancreatic glutamic-pyruvic transaminase, blood cell count, serum PO activity and the total protein content in stomachsand intestinal tracts of the litopenaeus vannamei.

The invention discloses an oplopanaxelatusNakai chlorogenic acid extract and a preparation method and application thereof. The method comprises the steps that an adventitious root material of oplopanaxelatusNakai is obtained by combining a tissue culture means and utilizing a bioreactor, and the chlorogenic acid extract of oplopanaxelatusNakai is obtained through macroporous adsorption resin extraction and separation. The adventitious root chlorogenic acid extract of oplopanaxelatusNakai can significantly protect liver injury caused by acetaminophen, the liver necrosis degree is obviously reduced, the levels of glutamic-pyruvic transaminase and glutamic oxalacetic transaminase can be significantly reduced, mitochondrial oxidative stress reaction caused by liver injury caused by excessive acetaminophen can be reduced, the activity of superoxide dismutase, catalase and glutathione in the liver is obviously restored, the content of malondialdehyde is reduced, the drug metabolism of acetaminophen in the liver is affected, and therefore the aseptic inflammatory injury of neutral granulocytes to the liver is reduced, the liver is protected, and liver injury is reduced.

The invention provides a stable reagent for measuring aspartate aminotransferase. The reagents of the present disclosure are dual reagents comprising TRIS, NADH, LDH, MDH, EDTA, Triton X-100, BSA, Proclin. The disclosed reagent can effectively improve the thermal stability, airborne stability and transportation stability of the NADH indicator system in vitro diagnostic reagent, and can effectively prolong the storage time of the kit.

The invention relates to application of gentiopicroside in preparation of medicines for treating non-alcoholic fatty liver diseases. Experiments prove that the gentiopicroside can achieve the following effects in non-alcoholic fatty liver diseases caused by fructose: obviously limiting increase of mouse weight and decrease of food ration, lowering triglycerides (TG), uric acid (UA) and glucose (GLU) as well as levels of serum glutamic oxalacetic transaminase (AST) and glutamic-pyruvic transaminase (ALT), obviously alleviating vacuolar degeneration of hepatic cells and deposition of fatty acidscaused by fructose, lowering levels of tumor necrosis factors (TNF-alpha) and interleukin 6 (IL-6) in hepatic tissues, raising levels of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px)in the hepatic tissues and lowering levels of malondialdehyde (MDA) and superoxide anions (O2<->). Results show that the gentiopicroside can achieve the effects of obviously promoting metabolism of the liver on fat, obviously improving and increasing the antioxidant stress level of the liver and alleviating hepatocellular injury, so that the aim of treating the fatty liver diseases is achieved.

The invention discloses a preparation method of earthworm powder. The preparation method includes the following steps: cleaning and disinfecting earthworms, and performing homogenization and freezing;performing re-homogenization and re-freezing after thawing; and performing vacuum freeze-drying on the re-frozen homogenate so as to prepare the earthworm powder. The prepared earthworm powder can significantly reduce the levels of total cholesterol, triglyceride and low-density lipoprotein cholesterol in patients with hyperlipidemia and can also reduce the level of glutamic oxaloacetic transaminase and glutamic-pyruvic transaminase to normal level without affecting liver functions; and the earthworm powder can improve blood viscosity, and finally, the situations of blood supply of body tissue can be improved so as to make blood vessels healthier.

The invention relates to application of Wangshi Baochi pills in preparation of a medicine for preventing and / or treating alcoholic liver injury, and belongs to the technical field of medicines. The application of the Wangshi Baochi pills in preparation of the medicine for preventing and / or treating alcoholic liver injury is provided, and researches find that the Wangshi Baochi pills can remarkably shorten the sobering time of drunken mice and reduce the levels of glutamic oxalacetic transaminase and glutamic-pyruvic transaminase in serum of acute alcoholic liver injury modeling mice, can significantly improve the pathological change of liver tissue of the acute alcoholic liver injury modeling mice, can significantly reduce the expression of active oxygen in the liver tissue of the acute alcoholic liver injury modeling mice, and can significantly improve the activity of superoxide dismutase and the level of glutathione in the liver tissue of the acute alcoholic liver injury modeling mice, and in addition, the Wangshi Baochi pills can increase the expression of quinone oxidoreductase in liver tissue of the acute alcoholic liver injury modeling mice and have a good alcoholic liver injury relieving effect.

The invention provides a Chinese patent medicine for treating rise of glutamic oxalacetic transaminase and glutamic-pyruvic transaminase and a preparation method of the Chinese patent medicine. The Chinese patent medicine comprises the following components in parts by weight: 500-2000 parts of radix sophorae flavescentis, 500-1000 parts of lysimachia christinae hance, 500-1000 parts of talc, 200-500 parts of liquorice, 500-1000 parts of plantain herb and 300-1000 parts of salviae miltiorrhizae. The Chinese patent medicine for treating rise of glutamic oxalacetic transaminase and glutamic-pyruvic transaminase provided by the invention can quickly discharge intrahepatic damp and heat, so that the liver function can get right within a short time. The Chinese patent medicine for treating rise of glutamic oxalacetic transaminase and glutamic-pyruvic transaminase provided by the invention is fast to take effect, good in curative effect and less in toxic and side effects to a human body.

The invention relates to an application of 4-hydroxyisoleucine in preparation of weight losing drugs or non-alcoholic fatty liver disease protecting drugs or healthcare food, that is, an application of the 4-hydroxyisoleucine in preparation of drugs for treating obesity and non-alcoholic fatty liver diseases. Oral preparations, capsules, tablets, granules and the like can be produced. Experiment find that 4-hydroxyisoleucine can effectively reduce weight, gathering of fat in the body, content of total cholesterol, triglyceride and low density lipoprotein in plasma and glutamic oxalacetic transaminase, protect the liver function, relieve fatty liver, reduce accumulation of liver lipid and safely and effectively reduce weight, reduce lipid and protect the liver for groups requiring obesity treatment.

The invention relates to application of ginsenoside Rg1 to preparation of a bilirubin rising treating preparation. In a single drinking experiment, the Rg1 can obviously reduce the content of triglyceride in blood; in a heavy drinking model, the Rg1 can obviously improve the survival rate of a drinking mice, reduces the content of glutamic pyruvic transaminase and glutamic oxalacetic transaminase in blood serum, reduces the content of cholesterol, the triglyceride and bilirubin in blood, and the like.

The invention discloses a new medicinal application of a Naoxintong preparation, and particularly relates to an application of the Naoxintong preparation in preparation of a medicine for treating non-alcoholic fatty liver. Experiments prove that the Naoxintong preparation can significantly reduce the body weight, liver weight / body weight index and liver lipid deposition of non-alcoholic fatty liver model animals, significantly improve liver function indexes including aspartate transaminase (AST) and glutamic-pyruvic transaminase (ALT), significantly reduce the levels of triglyceride (TG), cholesterol (TC) and low density lipoprotein (LDL-C) in serum in vivo, and significantly improve the liver function indexes of non-alcoholic fatty liver model animals. The traditional Chinese medicine composition has a remarkable effect on treating the non-alcoholic fatty liver disease. The medicinal range of the Naoxintong preparation is further expanded.

The invention provides application of a compound shown in a formula I in preparation of a drug for treating obesity and obesity-related symptoms, wherein R1, R2 and R3 are the same or different and are respectively selected from halogen elements; R4 is substituted or unsubstituted C1-C10 straight-chain or branched-chain alkyl; and R5 is a basic group. The research shows that the compound shown inthe formula I can effectively reduce the weight of high-fat diet-induced obese mice, OB mice and db / db mice, reduce the activity of glutamic-pyruvic transaminase ALT and glutamic oxalacetic transaminase AST and improve the fatty liver symptom. Therefore, the compound shown in the formula I has an improvement effect on obesity caused by bad dietary habits and obesity caused by genetic factors, andimproves the obesity-related symptoms such as fatty liver.

The invention provides an application of a pharmaceutical composition, the active ingredient of which is at least one of four polypeptides isolated and extracted from soybean or pea, and these four polypeptides can significantly reduce triglyceride, alanine aminotransferase ALT and aspartate aminotransferase AST levels, while reducing fat content and liver fat in patients with non-alcoholic fatty liver disease, improving insulin resistance. The composition is applied to prevent and treat non-alcoholic fatty liver and obesity medicine and health food.

The invention provides a composition containing puerarin, application of the composition, and belongs to the technical field of liver protection. The composition comprises the following ingredients inparts by weight: 1-2 parts of puerarin, 1-2 parts of curcumin and 1-2 parts of seabuckthorn flavone. The composition provided by the invention lowers a glutamic oxalacetic transaminase level, a glutamic-pyruvic transaminase level, a malonaldehyde level and a triglyceride level, and improves the levels of superoxide dismutase and reduced glutathione so as to protect liver injury.

The invention discloses a feed additive for freshwater fish. The feed additive is prepared by extracting platycarya strobilacea infructescence. The additive is applied to freshwater fish culture according to the addition amount of 100-10000 g / t, so that the growth of freshwater fish can be remarkably promoted, the feed coefficient is reduced, the fatness and the serum superoxide dismutase activityare improved, the content of malondialdehyde and nitric oxide in serum is reduced, and the superoxide anion and glutamic oxalacetic transaminase resisting activity is improved. Not only is productionperformance improved, but also fish meat quality is improved. The feed additive does not contain antibiotics, is healthy and environment-friendly, is low in production cost and has a great application prospect.

The invention discloses a method and feed for estimating the optimal energy-protein ratio, and the method comprises the following steps: (1) taking common feed raw material corn-soybean meal as basic ration, and blending bran and soybean oil into compound feed with different metabolic energy AME levels; (2) crushing and uniformly mixing all the raw materials, adding 5% of water, and granulating for later use; (3) adopting a single-factor complete random test design, and selecting healthy test ducks for feeding; (4) calculating the production performance in the feeding stage; at the end of the test, at least one duck with the weight close to the average weight is selected for a slaughtering test every time, and the slaughtering performance is measured; plasma is collected, and glutamic-pyruvic transaminase AST and glutamic oxalacetic transaminase AST are measured. And (5) calculating the optimal energy protein ratio according to a linear broken line regression model (y = l + u * (r-x)). According to the method, the optimal energy-protein ratio is estimated by establishing the regression model of the energy-protein ratio, the production performance and the slaughter performance, the result is more accurate, the application value is higher, and efficient utilization of feed resources is achieved.

The invention provides a mesenchymal stem cell preparation. Mesenchymal stem cells are cultured and pretreated by sequentially adopting a first culture medium for normal culture and a second culture medium containing prostaglandin E2. A mouse model animal experiment detection result shows that the mesenchymal stem cell preparation prepared by the invention can effectively reverse liver cirrhosis, the secretion level of glutamic oxalacetic transaminase in serum is remarkably reduced, meanwhile, the liver tissue dyed by a slice HE of the liver tissue is obviously recovered, and the deposition of collagen fibers in the liver tissue is also remarkably reduced, so that the mesenchymal stem cell preparation has the development trend towards the normal liver level.

The invention relates to a red-increasing and growth-promoting additive for parrot fish. The additive comprises carophyll red and vitamin E at the weight ratio of (7-15):(3-10). According to the invention, carophyll red and vitamin E which are adopted as raw materials are mixed into common feed at the ratio, and then the feed is conventionally used for feeding. Experiments prove that the surface of the parrot fish is obviously colored after the use of the additive, and the coloring effect is far greater than that of a control group; activities of glutamic-pyruvic transaminase and glutamic oxalacetic transaminase are not influenced so that no side influences occur to liver-pancreas of the parrot fish; moreover, the weight increment of the parrot fish and the activities of antioxidant indexes in the liver-pancreas are obviously influenced; in conclusion, the additive can effectively increase the color of the surface of the parrot fish and promote the growth of the parrot fish and has good effect in the increment of the antioxidant ability of the parrot fish and the accumulation of carotenoid.

The invention discloses a gene fragment for enhancing detoxification function and a transformed HepG2 cell, wherein the gene fragment for enhancing detoxification function is composed of three gene fragments of OTC, ARG1 and OATP1B1 in sequence. In HepG2 cells transformed with this gene fragment, the expression of albumin was doubled; the expression of aspartate aminotransferase was increased by 10%; the secretion of key proteins related to endogenous detoxification ability in the cytochrome P450 family was increased, and the detoxification ability was improved: the secretion of CYP2C9 was increased by 5 times, CYP2D6 secretion increased by 50 times, CYP2A6 secretion increased by 2 times, and CYP3A4 secretion increased by 100 times.

The invention provides novel GVS series compounds and applications thereof, which can obviously improve non-alcoholic fatty liver and fat accumulation. The medicine of the present invention can (a) improve the effects of non-alcoholic liver inflammation and fat accumulation under the condition of maintaining normal body weight; (b) significantly reduce aspartate aminotransferase (AST), alanine aminotransferase (ALT), malondialdehyde (MDA) while increasing the activity of superoxide dismutase (SOD); (c) reducing low-density lipoprotein cholesterol (LDL-C), triglyceride (TG) and increasing high-density lipoprotein cholesterol (HDL-C) (d) increase the content of adiponectin (ADP) in serum and reduce the activity of fatty acid synthase (FAS).

The invention relates to a feed additive capable of restoring a drug-induced liver injury of a grass carp. The feed additive is prepared from by glutathione, vitamin C, vitamin E, organic selenium, Chinese wolfberry fruits, astragalus membranaceus, soybean protein powder and wheat middling; the ingredients are reasonably matched in a certain proportion for producing out the feed additive, when the feed additive is added to grass carp feed, glutamic oxalacetic transaminase, glutamic-pyruvic transaminase and malonaldehyde which rise to a high level in the body of the grass carp can be adjusted to the normal level, meanwhile, a good restoration effect is achieved on liver tissue pathological changes caused by drug-induced elements, and the feed additive is simple in preparation method.

The invention discloses application of alanyl-glutamine (Ala-Gln) in preparation of a medicine for preventing and treating acute liver failure. The application is characterized in that the alanyl-glutamine or pharmaceutically acceptable salt, ester and solvate thereof is applied to prevention and treatment of an acute immune mouse liver failure model induced by Lipopolysaccharide (LPS) combined with D-galactosamine (D-Gal). Pharmacological experiments find that the alanyl-glutamine administration can obviously improve the liver tissue structure disorder, reduce extravasated blood and edema of liver tissues, reduce glutamic-pyruvic transaminase, glutamic oxalacetic transaminase and other liver function indexes and inhibit inflammatory reactions in the liver. The application has the advantage that the alanyl-glutamine provides a new method for preventing and treating LPS / D-Gal induced acute liver failure and liver injury.

The invention belongs to the field of biomedical research, in particular to the application of NADPH and vitamin E in combination for preparing a medicine for preventing or treating liver damage. NADPH and vitamin E can play a synergistic effect within a certain dose range, and have a good protective effect on liver damage caused by carbon tetrachloride, not only can significantly reduce aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphate Enzyme (ALP) and total bilirubin (TB) content, and can increase the content of ATP in hepatocytes, reduce the levels of MDA in hepatocytes and serum, increase the levels of SOD and GSH in hepatocytes and serum, and increase the levels of SOD and GSH in hepatocytes. CAT level. It was also proved by cell experiments that the combination of NADPH and vitamin E could significantly reduce the decrease in the survival rate of liver cells in the liver injury model induced by CCl4, thereby playing a role in preventing or treating liver injury.

The invention discloses an application of recombinant collagen in preparation of a hemostatic material. The recombinant collagen produced by fermenting pichia pastoris with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.5021 is adopted, so that the tissue injury bleeding time can be remarkably shortened, the bleeding amount can be remarkably reduced, and the pathological injury of the liver can be remarkably relieved; the content of glutamic oxalacetic transaminase, glutamic-pyruvic transaminase, lactic dehydrogenase, interleukin 6, tumor necrosis factor alpha, transforming growth factor-beta, direct bilirubin and total bilirubin caused by the liver injury is reduced, and the traditional Chinese medicine composition has relatively good anti-hepatic hemorrhage and relatively good liver injury repairing effects.