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Application of coptisine or coptisine salts in preparation of medicines for preventing and treating fatty liver damage

A technology of fatty liver and coptisine, applied in the field of pharmacy, to achieve definite curative effect, improve lipid metabolism, and reduce drug efficacy

Active Publication Date: 2014-03-12
QINGDAO BAILI CAIXIN MEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there are no reports on the effects of coptisine on inhibiting liver lipid accumulation, preventing fatty liver formation and resisting fatty liver damage.

Method used

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  • Application of coptisine or coptisine salts in preparation of medicines for preventing and treating fatty liver damage
  • Application of coptisine or coptisine salts in preparation of medicines for preventing and treating fatty liver damage
  • Application of coptisine or coptisine salts in preparation of medicines for preventing and treating fatty liver damage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Inhibitory effect of coptisine on triglyceride accumulation in HepG2 hepatocytes

[0028] 1. The effect of coptisine on the proliferation activity of liver cells

[0029] Routinely culture HepG2 cells until 80% of the culture dish is covered. Coptisine is dissolved in DMSO and PBS and added to the cultured cells according to the determined difference. After acting on HepG2 cells for 24 hours, the MTT test shows that the survival rate of the cells increases with that of Coptisine. The effect concentration increased and decreased (Table 1); when the effect concentration of coptisine was 50.0 μg / mL, the survival rate of liver cells was significantly reduced (P0.05); it shows that coptisine has a certain effect on the growth of HepG2 cells in the concentration range of 0-10.0 μg / mL. The influence is very small, and it is safe to choose the administration concentration within this concentration range.

[0030] Table 1 The effect of Coptisine on the growth activi...

Embodiment 2

[0041] Example 2 Effect of Coptisine on Dyslipidemia in Experimental Rats

[0042] Experimental animals: male SD (Sprague Dawley) rats, SPF grade, body weight 180±20 g.

[0043] Experimental method: After one week of adaptive feeding, the rats were randomly divided into normal control group and model group according to body weight, of which 10 in the normal control group were fed with basal diet; rats in the model group were fed high-fat diet for 20 days and then retro-orbital Blood was collected from the venous plexus, and compared with before feeding high-fat diet, the TC and TG in the blood of the model group were measured, and the significantly increased rats were selected as the rats with successful hyperlipidemia modeling.

[0044] Those whose serum TG increase value was greater than 50% were included in the experiment, and the hyperlipidemia rats were divided into hyperlipidemia model group, positive drug simvastatin, gemfibrozil group, coptisine hydrochloride, coptis...

Embodiment 3

[0053] Example 3 Effect of Coptisine on Hydrocortisone-Induced Fatty Liver in Rats

[0054] Excessive use of cortisol drugs is one of the important causes of drug-induced fatty liver. In this study, adult male rats were subcutaneously injected with hydrocortisone (36mg / kg) to reproduce the drug-induced fatty liver model induced by excessive cortisol drugs. Different doses of HLJ were used for prevention and treatment, and tiopronin and The comparative study of Gan Tai Le aims to investigate the curative effect of coptisine on fatty liver disease induced by excessive use of cortisol drugs, and to provide a scientific basis for the further development and research of coptisine.

[0055] 1. Materials and methods

[0056] (1) Drugs: coptisine hydrochloride (HLJY), provided by Sichuan Institute of Natural Plants, batch number: 130106; coptisine (HLJ), provided by Guangdong Institute of Traditional Chinese Medicine, batch number: 120503; the positive drug Tioprol Ning (provided ...

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Abstract

The invention discloses an application of coptisine or coptisine salts in the preparation of medicines for preventing and treating fatty liver damage. The pharmacological research proves that the coptisine has the effects of lowering lipid, improving lipid metabolism and alleviating the hepatic lesion caused by hepatic tissue fat accumulation and is capable of inhibiting hepatocyte triglyceride accumulation; for experimental hyperlipemia symptoms, the coptisine has the effects of obviously reducing triglyceride, cholesterol and LDL-C (low-density lipoprotein cholesterol) in blood and reducing the content of triglyceride in hepatic tissues; for fatty liver caused by the overuse of cortisol, the coptisine is capable of reducing the accumulation of triglyceride in the liver, the rise of activity of glutamic-pyruvic transaminase (GPT) and glutamic oxalacetic transaminase (GOT) and alleviating the hepatic lesion and has an excellent fatty liver prevention effect; part indexes of the coptisine are obviously superior to those of lipid reducing medicines, namely simvastatin and fenofibrate and liver protecting medicines, namely tiopronin and glucurolactone; the coptisine can be applied to clinical fatty liver diseases.

Description

technical field [0001] The invention belongs to the field of pharmacy, and in particular relates to the application of a natural compound coptisine or a salt thereof in the preparation of a drug for preventing and treating fatty liver injury. Background technique [0002] The liver is an important place for fat metabolism. Excessive accumulation of lipids (mainly triacylglycerols) in the liver, more than 5% of the liver's weight, or more than 50% histologically, when the liver's ability to synthesize fat and / or reduce its ability to transport into the blood When the liver parenchyma becomes fatty, it is fatty liver (osteoporosis). Fatty liver is not an independent disease, but fatty degeneration of the liver caused by various diseases and reasons. The most common causes are obesity, alcoholism, and diabetes; followed by malnutrition, drug poisoning, pregnancy, and genetics. Fatty liver is a common disease among middle-aged and elderly people. With the continuous improvem...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/4375A61P1/16A61P3/06A61P3/10A23L1/30A23L33/10A23L33/105
Inventor 郭姣范辉贝伟剑
Owner QINGDAO BAILI CAIXIN MEDICAL TECH CO LTD
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