Application of recombinant collagen in preparation of hemostatic material

A technology for recombining collagen and hemostatic materials, which is applied in the field of medical materials to achieve large specific surface area, reduce bleeding time and amount, and effectively resist hemorrhage

Pending Publication Date: 2022-07-01
JIANGSU JLAND BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Collagen from animal sources faces immunogenicity problems during use, limiting its clinical application

Method used

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  • Application of recombinant collagen in preparation of hemostatic material
  • Application of recombinant collagen in preparation of hemostatic material
  • Application of recombinant collagen in preparation of hemostatic material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] 1. Hemostasis experiment of recombinant collagen on rat liver hemorrhage model

[0019] (1) 48 SD rats, male, were randomly divided into 4 groups with 12 rats in each group, namely blank group, test drug (recombinant collagen) group, model group and positive control group.

[0020] (2) After fasting for 12 hours, the rats were anesthetized by intraperitoneal injection of 3% chloral hydrate (1 mL / 100 g), the abdominal skin was prepared, and the surgical area was disinfected with 5% iodine.

[0021] (3) Make a 2.5cm longitudinal incision in the abdomen of the rat with a scalpel, separate layer by layer, fully expose the right lobe of the liver, and cut a 1cm×0.2cm×0.3cm liver tissue at the lower edge of the right hepatic lobe.

[0022] (4) Prepare sterile gauze in advance, and use an electronic balance to measure the initial weight of the gauze.

[0023] (5) After the liver tissue was removed, the model group immediately used sterile gauze to suck out the blood, and turn...

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PUM

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Abstract

The invention discloses an application of recombinant collagen in preparation of a hemostatic material. The recombinant collagen produced by fermenting pichia pastoris with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.5021 is adopted, so that the tissue injury bleeding time can be remarkably shortened, the bleeding amount can be remarkably reduced, and the pathological injury of the liver can be remarkably relieved; the content of glutamic oxalacetic transaminase, glutamic-pyruvic transaminase, lactic dehydrogenase, interleukin 6, tumor necrosis factor alpha, transforming growth factor-beta, direct bilirubin and total bilirubin caused by the liver injury is reduced, and the traditional Chinese medicine composition has relatively good anti-hepatic hemorrhage and relatively good liver injury repairing effects.

Description

technical field [0001] The invention belongs to the technical field of medical materials, and relates to the application of recombinant collagen in the preparation of hemostatic materials. Background technique [0002] Human hemostasis mechanisms include vasoconstriction, platelets, and blood coagulation. Vasoconstriction and platelet aggregation locally form thrombus and release a large number of procoagulants to coagulate blood to stop bleeding. Mild bleeding can be stopped by compression, but some, such as severe trauma bleeding during medical surgery, are almost impossible to stop bleeding by compression and natural repair of the human body. [0003] Hemostatic powder is a powdery material that can absorb tissue exudates and combine with hemostatic components in blood, and has good hemostatic repair performance. At present, the commonly used hemostatic powder is mainly chitosan hemostatic powder. Although chitosan can stop bleeding quickly, inflammatory cell infiltrati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L24/10A61L24/00
CPCA61L24/102A61L24/0036A61L24/0015A61L24/001A61L2400/04A61L2300/252A61L2300/418
Inventor 赵健烽朱逸丽范蕾陈淑婷黄建民
Owner JIANGSU JLAND BIOTECH CO LTD
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