The invention discloses a reagent for determining the content of human cholyglycine by using a latex immunoturbidimetry technology. The reagent is prepared from a reagent body 1 and a reagent body 2,wherein the reagent body 1 is prepared by adding 100 mM of an auxiliary agent, a phosphate buffer solution with the pH value of 8.0, a sodium chloride solution with the concentration of 0.9%, BSA withthe concentration of 0.1% and a stabilizer into a latex microsphere-BSA-cholyglycine conjugate with the concentration of 0.04%, and the reagent body 2 is prepared by adding 15 mM of an auxiliary agent, a phosphate buffer solution with the pH value of 7.4, a sodium chloride solution with the concentration of 0.9%, BSA with the concentration of 0.1%, a surfactant with the concentration of 0.1% anda stabilizer into an anti-mouse cholyglycine monoclonal antibody with the concentration of 5%. According to the reagent, latex microspheres are introduced into the reagent, due to the existence of thelatex microspheres, the sensitivity of the detection reagent is greatly improved, and the requirements of clinical use are met. Compared with homogeneous enzyme immunoassay, the reagent has great advantages in stability, under the acceleration condition, and the stability time of the reagent is at least 2 times or above of the stability time of a homogeneous enzyme immunoassay reagent.