39 results about "Furaltadone" patented technology

A method for detecting furantadone by using single clone antibody as fixator includes using single clone antibody resisting furantadone as fixator to set up ELISA fast detection manner for detecting furantadone residue and utilizing said manner to prepare detection kit and detection test paper for providing fast means to detect out furantadone residue.

The invention discloses a monoclonal antibody capable of identifying a furaltadone residue marker 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ). The monoclonal antibody is secreted by a hybridoma cell AMOZ; and the hybridoma is preserved in China Center for Type Culture Collection, and has a preservation number of CCTCC NO:C201188. The invention also discloses an enzyme immunoassay method and a reagent kit for detecting furaltadone marker residue marker AMOZ, and application thereof to detection of the furanketone residue marker AMOZ. Compared with prior art, the monoclonal antibody prepared by the invention, the ELISA Kit and the ELISA method have high detection sensitivity, high precision and good accuracy; a hapten synthesis process is simple, and has high synthesis efficiency; and a derivatization reagent for sample pretreatment is benzene formaldehyde, which has advantage of small toxicity compared with other commonly used derivative reagent like o-nitrobenzaldehyde.

The invention discloses a method for determining residues of metabolites of four nitrofurans drugs in an animal glue traditional Chinese medicine. The method comprises the following steps: preparing to-be-tested sample solution; preparing standard solution; injecting a standard series of working solution into a liquid chromatograph / mass spectrometer, determining appearance positions of the metabolites of the four nitrofurans drugs and qualitative and quantitative determination ion pairs thereof, and making a standard curve equation by taking the abundance of the quantitative ion pairs as a vertical coordinate and taking the concentration as a horizontal ordinate; substituting data obtained by corresponding detection for the to-be-tested sample solution into the standard curve equation forcalculation, so as to obtain the contents of the metabolites of all the nitrofurans drugs in the to-be-tested sample. The metabolites of the four nitrofurans drugs are: a furanzolidon metabolite (AOZ), a furaltadone metabolite (AOMZ), a furacilin metabolite (SEM) and a furadantin metabolite (AHD).

The invention discloses an arborescence half antigen capable of directly detecting furaltadone metabolite AMOZ, an arborescence antigen and application of the arborescence half antigen. The arborescence half antigen is obtained by coupling glyoxylic acid derivative of AMOZ to an active amino terminal of four-branch polyamide-amine, an artificial antigen is prepared based on the arborescence half antigen, and then an antibody is prepared by virtue of the artificial antigen; By adopting the artificial antigen disclosed by the invention, a high-quality antibody directly aiming at AMOZ can be obtained, valence of the antibody is 1:64000, the lowest detection limit of a chemiluminiscence enzyme linked immunosorbent assay (CLEIA) analytical method established based on the antibody is 0.112ng / ml, 50% inhibiting concentration is 15.26ng / ml, the CLEIA analytical method can be directly applied to detection of AMOZ, and the defects of the traditional scheme that an antibody prepared by virtue of an AMOZ half antigen and the artificial antigen is poor in antibody specificity and can not directly identify the AMOZ. The arborescence half antigen, the artificial antigen and the antibody have a wide application prospect in detection of AMOZ residue in food, and a preparation thinking of the half antigen can provide a reference for design of half antigens of other similar small molecule compounds.

The invention discloses a fast immunological detection method for directly detecting furaltadone metablolite 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ). The method comprises preparation and application of an AMOZ half antigen, an artificial antigen, an antibody and the like. The AMOZ half antigen has a molecular structure shown as a formula (I) or a formula (II), and the AMOZ artificial antigen has a molecular structure shown as a formula (III) or a formula (IV). An antibody prepared from the antigen can be used for specifically identifying AMOZ. The valence of the antibody is 1:64000, the lowest detection limit is 0.852ng / mL, and the half inhibiting concentration is 15.26ng / mL. The antibody can be directly applied to the detection of AMOZ. By adopting the method, the defect of derivatization of AMOZ in the conventional enzyme linked immunosorbent assay method is overcome; the method is easy, convenient, rapid and low in cost. The antigen and the antibody can be widely applied to residue detection of AMOZ in foods.

The invention discloses a preparation method of a quality control sample of nitrofuran metabolite residues in fish muscle. The preparation method comprises the following steps: selecting negative living fish which meets pre-set specification and size and is not fed by a nitrofuran medicine; carrying out medicine bath treatment under pre-set conditions, wherein a water body of a medicine bath tankcontains four types of nitrofuran drugs including a furazolidone crude drug, a furaltadone crude drug, a furacilin crude drug and a furantoin crude drug; fishing out a positive sample subjected to themedicine bath treatment and killing the fish and taking back muscle to obtain a fish muscle sample; adding a vitamin C solution and uniformly stirring to obtain minced fish; after pre-cooling the minced fish, putting into a freezing drying machine and carrying out freezing drying to obtain a freeze-dried sample; carrying out crushing, blending and sieving treatment on the freeze-dried sample to obtain the quality control sample containing nitrofuran metabolite residues with a target content level. By adopting the scheme, the quality control sample contains four types of nitrofuran metabolitesand can meet the requirements of actual inspection better.

The present invention provides an enzyme-linked immunoassay kit and a method for furaltadone metabolite detection. The enzyme-linked immunoassay kit comprises an enzyme label plate coated with coating antigen, specific furaltadone metabolite antibody, an enzyme marker, furaltadone metabolite standard solutions, a substrate coloration solution, a termination solution, a concentration washing solution, a concentration reconstituted solution, and 2-nitrobenzaldehyde. The present invention further provides a furaltadone metabolite enzyme-linked immunoassay kit detection method, which mainly comprises: carrying out a sample pretreatment, adopting the kit to detect, and finally analyzing a detection result. With the detection kit, furaltadone metabolite drugs in animal tissues, aquatic products and honey can be rapidly detected, characteristics of simple and rapid operation, high accuracy, high sensitivity, low cost and the like are provided, and the kit and the method are applicable for screening and on-site monitoring of a large number of samples.

The invention relates to a furaltadone metabolite chemiluminescent enzyme linked immune sorbent rapid analysis kit. The furaltadone metabolite chemiluminescent enzyme linked immune sorbent rapid analysis kit comprises a luminescence test plate, a furaltadone metabolite standard solution, a furaltadone metabolite monoclonal antibody working solution, an enzyme-labeled secondary antibody, a luminescence liquid, a sample diluent, and a washing concentrate. The furaltadone metabolite monoclonal antibody working solution is a solution of Balb-c mouse immune antibody, and the solution is diluted according to a volume ratio of 1:3500 for application, wherein a furaltadone metabolite-bovine serum albumin conjugate is taken as an immunogen of Balb-c mouse immune antibody; the enzyme-labeled secondary antibody is a goat anti mouse IgG working solution labeled by the furaltadone metabolite-horse radish peroxidase, and is diluted according to a volume ratio of 1:10000 for application; and the luminescence liquid comprises a luminescent substrate A, and a luminescent substrate B. The furaltadone metabolite chemiluminescent enzyme linked immune sorbent rapid analysis kit is high in sensitivity, wide in linear dynamic range, long in optical signal duration, rapid in analyzing, stable in results, less in error, high in safety, and long in usage period.

The invention provides an immunodetection method capable of detecting Furaltadone metabolite AMOZ, MG (malachite green) and LMG (leucomalachite green) simultaneously. An AMOZ hapten and an LMG hapten are synthesized scientifically and coupled with carrier proteins respectively, and artificial antigens are prepared. Hybridoma cell strains aiming at the two objects are prepared respectively from the AMOZ artificial antigen and the LMG artificial antigen, a hybrid-hybridoma cell strain capable of secreting an anti-AMOZ and anti-LMG bispecific antibody is prepared with a hybrid-hybridoma technique, a multi-residue immunodetection method is established successfully, simultaneous detection of AMOZ, MG and LMG is realized, the limit of detection of AMOZ is 0.1 ng / mL, and the IC50 is 1.3 ng / mL; the limit of detection of LMG is 4.8 ng / mL, and the IC50 is 45.3 ng / mL. The method has high specificity and higher sensitivity, has broad application prospect in multi-residue detection of AMOZ, MG and LMG in food and is simple and convenient to operate.