Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Aggregation-induced emission immunochromatography kit for detecting two nitrofuran metabolites and application thereof

A technology of aggregation-induced luminescence and immunochromatographic test paper, which can be used in material excitation analysis, measurement device, analysis material, etc. Effect

Pending Publication Date: 2022-07-29
BOHAI UNIV
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CN113156112A discloses a fluorescent immunochromatography kit for simultaneously detecting four nitrofuran metabolites and its preparation method and application. The method uses quantum dot fluorescent microsphere-labeled antibodies to detect nitrofuran drug metabolites Fluorescent immunochromatographic test strips, the markers used in the kit will undergo fluorescence quenching in the case of aggregation, that is, the aggregation-induced quenching effect. When the concentration of the target to be detected is low, this aggregation-induced quenching The labeling method of fluorescent materials still limits the detection of immunochromatographic test strips, which affects the detection accuracy and sensitivity

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Aggregation-induced emission immunochromatography kit for detecting two nitrofuran metabolites and application thereof
  • Aggregation-induced emission immunochromatography kit for detecting two nitrofuran metabolites and application thereof
  • Aggregation-induced emission immunochromatography kit for detecting two nitrofuran metabolites and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The aggregation-induced luminescence immunochromatography kit for detecting two nitrofuran metabolites includes immunochromatographic test strips and aggregation-induced luminescence fluorescent probes, such as figure 1 As shown, the immunochromatographic test strip includes a polyvinyl chloride bottom plate, a nitrocellulose membrane is arranged on the upper surface of the polyvinyl chloride bottom plate, and two left and right sides of the nitrocellulose membrane are respectively arranged on the upper surface of the polyvinyl chloride bottom plate. The water absorbing pad and the sample pad on the side are provided with a detection line T1, a detection line T2 and a quality control line C in sequence on the upper surface of the nitrocellulose membrane near the sample pad side;

[0044] The detection strip T1, the detection line T2 and the quality control line C were respectively coated with 2-NP-AMOZ-BSA with a concentration of 0.15 mg / mL at a rate of 1 μL / cm using a f...

Embodiment 2

[0095] When preparing the fluorescent microspheres, the ultrasonic action time at room temperature was 2 min. Others were the same as those in Example 1. The aggregation-induced luminescent fluorescent microsphere probes of the present invention could also be prepared for the detection of nitrofurantoin metabolites and nitrofurantoin metabolites.

Embodiment 3

[0097] When preparing the fluorescent microspheres, the ultrasonic action time at room temperature was 10 min. Others were the same as those in Example 1. The aggregation-induced luminescent fluorescent microsphere probes of the present invention could also be prepared for the detection of nitrofurantoin metabolites and nitrofurantoin metabolites.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle sizeaaaaaaaaaa
recovery rateaaaaaaaaaa
Login to View More

Abstract

The invention relates to an aggregation-induced emission immunochromatography kit for detecting two nitrofuran metabolites and application, the kit comprises an immunochromatography test strip and an aggregation-induced emission fluorescent probe, the immunochromatography test strip comprises a polyvinyl chloride base plate, the upper surface of the polyvinyl chloride base plate is provided with a nitrocellulose membrane, a water absorption pad and a sample pad, and the nitrocellulose membrane is arranged on the upper surface of the polyvinyl chloride base plate. A detection line T1, a detection line T2 and a quality control line C are sequentially arranged on one side, close to the sample pad, of the upper surface of the nitrocellulose membrane; the aggregation-induced emission fluorescent probe comprises a green aggregation-induced emission fluorescent microsphere probe prepared from BTPETD and a red aggregation-induced emission fluorescent microsphere probe prepared from TPB. The device has the advantages of convenience in use and simplicity in operation. The method is used for qualitative and quantitative detection of the furaltadone metabolite and the nitrofurantoin metabolite at the same time, the detection efficiency is greatly improved, and the detection sensitivity can be effectively improved.

Description

technical field [0001] The invention belongs to the technical field of antibiotic detection, relates to an aggregation-induced luminescence immunochromatography kit for detecting two nitrofuran metabolites and its application, in particular to a fluorescent immune layer prepared by using aggregation-induced luminescence fluorescent microspheres as a labeled carrier Analytical test strips for simultaneous detection of nitrofurantoin metabolites (AMOZ) and nitrofurantoin metabolites (AHD) in aquatic products. Background technique [0002] Nitrofurans have been widely used in livestock and poultry and aquaculture because of their low price and good effect, for the treatment of gastrointestinal infections and the prevention of microbial infections caused by lesions. Because nitrofurans and their metabolites have side effects such as carcinogenicity and mutagenicity to humans, many countries, including my country, have long banned their use in animal food production. Nitrofuran ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/533G01N33/543G01N33/558G01N21/64
CPCG01N33/5308G01N33/558G01N33/533G01N33/54313G01N21/6428G01N2021/6432
Inventor 刘秀英夏菲范玲朱力杰励建荣李学鹏张少恩郭晓华高雪程媛媛
Owner BOHAI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com