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Kit for detecting furaltadone metabolites in food

A detection kit and furaltadone technology, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of inability to realize rapid detection of a large number of samples, cumbersome operation, high cost, etc., and achieve short reading time and simple operation , quick effect

Inactive Publication Date: 2017-05-10
BEOSON JIANGSU FOOD SAFETY TECH CO LTD
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  • Abstract
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  • Application Information

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Problems solved by technology

[0004] At present, the detection methods of furaltadone metabolites are mainly high-performance liquid chromatography, liquid mass spectrometry and other ultraviolet instrument methods, but these methods have the disadvantages of cumbersome operation, time-consuming, expensive, etc., and cannot realize the rapid detection of a large number of samples

Method used

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Embodiment 1

[0020] In an embodiment of the present invention, a detection kit for furaltadone metabolites in food, comprising: a microtiter plate coated with furaltadone metabolite antigen, furaltadone metabolite monoclonal antibody, green peroxidase Labeled goat anti-mouse anti-antibody, standard solution, substrate chromogenic solution, stop solution, concentrated washing solution, concentrated complex solution.

[0021] The furaltadone metabolite antigen is a conjugate of furaltadone metabolite hapten and keyhole limpet hemocyanin; the preparation steps of furaltadone metabolite hapten are as follows: 5g furaltadone metabolite and 25-30ml dimethyl Add the mixed solution of phenylenediamine slowly at room temperature to 75-85ml of dimethylphenylenediamine solution containing 3.5-4.2g of dimethylaminobenzaldehyde. ~1.5h, remove the solvent, and purify by column chromatography to obtain. Green peroxidase-labeled goat anti-mouse anti-antibody is obtained by coupling the labeling enzyme gr...

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Abstract

The invention discloses a kit for detecting furaltadone metabolites in a food. The kit comprises an ELISA plate coated with a furaltadone metabolite antigen, a furaltadone metabolite monoclonal antibody, a verdoperoxidase-labelled goat anti-mouse antiantibody, a standard solution, a substrate developing solution, stop buffer, concentrated scrubbing solution and concentrated reconstitution fluid. The furaltadone metabolite antigen is a conjugate of furaltadone metabolite half antigen and keyhole limpet haemocyanin; and the verdoperoxidase-labelled goat anti-mouse antiantibody is obtained by coupling marker enzyme verdoperoxidase and a goat anti-mouse antiantibody through a glutaraldehyde crosslinking method. The kit is high in sensitivity and stability and suitable for aquatic products such as fishes and shrimps and animal products such as chicken and pork.

Description

technical field [0001] The invention relates to the technical field of food detection, in particular to a detection kit for furaltadone metabolites in food. Background technique [0002] Nitrofuran spectrum antibiotics (furaltadone, furazolidone, nitrofurantoin, nitrofurazone) mainly appear in animal foods. They have harmful effects on human health and are carcinogenic. Many countries including the United States, Canada and the European Union have banned the use of food. These countries have a ban on all imported foods that contain nitrofuran residues. [0003] Since furaltadone metabolites are not easily decomposed in general cooking and can be released from tissues under mild acidic conditions, they can be used for detection. For the safety of people and animals, it is necessary to carry out such antibiotic residue testing on water sources and food (such as: meat). [0004] At present, the detection methods of furaltadone metabolites are mainly high-performance liquid c...

Claims

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Application Information

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IPC IPC(8): G01N33/535G01N33/558G01N33/577
CPCG01N33/535G01N33/558G01N33/577
Inventor 张进吴念绮周朱晨张根义胡彬朱倩倩
Owner BEOSON JIANGSU FOOD SAFETY TECH CO LTD
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