114 results about "Toxic proteins" patented technology

Disclosed are Bacillus thuringiensis strains comprising novel crystal proteins which exhibit insecticidal activity against coleopteran insects including red flour beetle larvae (Tribolium castaneum) and Japanese beetle larvae (Popillia japonica). Also disclosed are novel B. thuringiensis crystal toxin genes, designated cryET33 and cryET34, which encode respectively the coleopteran-toxic proteins, CryET33 (29-kDa) crystal protein, and CryET34 (14-kDa) crystal protein. Also disclosed are methods of making and using transgenic cells comprising the novel nucleic acid sequences of the invention.

The invention provides a method of propagating an adenoviral vector. The method comprises (a) providing a cell comprising a cellular genome comprising a nucleic acid sequence encoding a tetracycline operon repressor protein (tetR), and (b) contacting the cell with an adenoviral vector comprising a heterologous nucleic acid sequence encoding a toxic protein. The heterologous nucleic acid sequence is operably linked to a promoter and one or more tetracycline operon operator sequences (tetO), and expression of the heterologous nucleic acid sequence is inhibited in the presence of tetR, such that the adenoviral vector is propagated. The invention also provides a system comprising the aforementioned cell and adenoviral vector.

The present invention provides an improved process for the production of recombinant peptides by fusion of recombinant peptides with icosahedral viral capsids and expression of the fusion in bacterial cells of Pseudomonad origin. The Pseudomonad cells support formation of virus like particles from icosahedral viral capsids in vivo, and allow the inclusion of larger recombinant peptides as monomers or concatamers in the virus like particle. The invention specifically provides cells expressing viral capsid fusions, nucleic acids encoding fusions of toxic proteins with icosahedral viral capsids and processes for manufacture of recombinant proteins.

The present application relates to recombinant microorganisms useful in the biosynthesis of unsaturated C6-C24 fatty alcohols, aldehydes, and acetates which may be useful as insect pheromones, fragrances, flavors, and polymer intermediates. The C6-C24 fatty alcohols, aldehydes, and acetates described herein may be used as substrates for metathesis reactions to expand the repertoire of target compounds and pheromones. The application further relates to recombinant microorganisms co-expressing a pheromone pathway and a pathway for the production of a toxic protein, peptide, oligonucleotide, or small molecule suitable for use in an attract-and-kill pest control approach. Also provided are methods of producing unsaturated C6-C24 fatty alcohols, aldehydes, and acetates using the recombinant microorganisms, as well as compositions comprising the recombinant microorganisms and / or optionally one or more of the product alcohols, aldehydes, or acetates.

The present invention relates to a system for expressing toxic proteins, to an expression vector comprising this system, to a prokaryotic cell transformed with this system, and also to a method for synthesizing a toxic protein using this expression system. The expression system of the invention is characterized in that it comprises successively, in the 5′-3′ direction, a nucleotide sequence encoding the Asp-Pro dipeptide and a nucleotide sequence encoding a toxic protein. According to a preferred embodiment of the invention, the expression system also comprises, upstream of the Asp-Pro sequence, a nucleotide sequence encoding a soluble protein. The expression system of the invention makes it possible to construct an expression vector that is useful for transforming a prokaryotic cell such as E. coli, for example in a method for synthesizing the toxic protein.

Disclosed herein are nucleotide sequences encoding an insecticidal protein exhibiting Lepidopteran inhibitory activity, as well as novel insecticidal proteins referred to herein as a BCW 001, BCW 002, BCW 003, and BCW toxic protein-containing chimeras and BCW toxin insecticide, transgenic plants expressing the chimeras or the insecticide, and methods for detecting the presence of the nucleotide sequences or the insecticide in a biological sample.

The invention discloses a method of determining bacillus thuringiensis toxic protein CrylAc. The method comprises the steps that: Fe3O4@Au nana-particles combining with a transgenic Bt toxic protein CrylAc primary antibody is modified onto the surface of a magnetic control glassy carbon electrode, and specific immune reaction is carried out on the magnetic control glassy carbon electrode with an antigen and a GOD labeled secondary antibody to form a compound with a sandwich structure; an electrogenerated chemiluminescence immunosensor is developed to be used for detecting the concentration of transgenic Bt toxic protein CrylAc; glucose is catalyzed and oxidized by using GOD to generate H2O2; voltage is applied to a working electrode to excite luminal to be oxidized; a luminal oxide and the H2O2 react to generate an electrochemiluminescence signal; an electrochemiluminescence response is strengthened along with the increase of the concentration of the GOD; and the electrochemiluminescence signal has a linear relation with the concentration of the transgenic Bt toxic protein CrylAc within a range of 0-6ng / mL. The method has high sensitivity and low detection limit, is widely applied and supplies a wide application prospect of reliably and ultra-sensitively detecting the toxic protein of a transgenic plant.

The invention discloses application of mercuride in preparing antivenom medicaments, aiming at solving the problems of unobvious treatment effect, high death rate and the like in the prior art, wherein the mercuride is one of mercuric sulfide, mercuric oxide, mercuric sulfate, mercuric chloride and the like. Because the main component in the mercuride is heavy metal which has the characteristic of inactivating all proteins, the mercuride has fatal destructive effect on all the proteins; and snake venom is formed by mixing dozens of poisonous proteins, therefore, the mercuride can inactivate the poisonous proteins in the snake venom so as to enable the poisonous proteins to lose activity (toxicity), thereby achieving the purpose of resisting the snake venom.

The invention discloses a hansenula polymorpha genetic operation strategy and application thereof. The hansenula polymorpha genetic operation strategy is characterized in that a DNA (Deoxyribonucleic Acid) fragment group used for knocking out a target gene of hansenula polymorpha consists of a DNA fragment A and a DNA fragment B; the DNA fragment A in the direction from end 5' to end 3' is structured as 5' end sequence of the target gene, m, mazF expression cassette, and part of resistance screening marker gene cassette fragment A, in sequence; the DNA fragment B in the direction from 5' end to 3' end is structured as part of resistance screening marker gene cassette fragment B, n, and 3' end sequence of the target gene, in sequence. According to the hansenula polymorpha genetic operation strategy, methanol induced toxic protein mazF has the effect of cutting mRNA (messenger Ribonucleic Acid), thus the purpose of knockout without a marker or a trace can be realized; the hansenula polymorpha genetic operation can be performed conveniently.

The invention relates to a method for inducing phytophthora capsici to generate toxic secretory proteins. The method comprises the following steps: bringing pepper phytophthora blight tissues back to a laboratory, scraping off the basal part surfaces of stems of diseased plants by a surgical knife blade at first, then washing the diseased plants by running water, taking 0.5cm of tissue blocks from disease-health junctions and placing the tissue blocks in a V8 culture medium, culturing for 3-4 days under a 25 DEG C dark condition until a large bacterial colony is formed, transferring a bacterial disk into a toxic protein liquid induction culture medium, carrying out shaking culture for 15-20 days, filtering mycelia by filter paper, centrifuging the filtrate for 30min at a speed of 8000rp, adding saturated ammonium sulphate in the supernatant, centrifuging the filtrate for 30min at a speed of 12000rp, carrying out a dialysis treatment on a precipitate, freeze-drying to obtain the toxic proteins secreted by the phytophthora capsici, and storing the toxic proteins at -70 DEG C. The method is capable of inducing the phytophthora capsici to generate lots of the toxic secretory proteins, thus filling in the gap that lots of toxic secretory proteins cannot be obtained in the domestic and overseas phytophthora capsici pathogenesis researches, and having great significance.