66 results about "Hansenula mrakii" patented technology

The invention discloses a brewing method of improving aroma characteristics of prickly pear fruit wine by non-saccharomyces cerevisiae. The non-saccharomyces cerevisiae and saccharomyces cerevisiae are adopted for mixed fermentation. The non-saccharomyces cerevisiae is one or more species of Hanseniaspora uvarum strain F119, Wickerhamomyces anomalus strain C11 and Hyphopichia burtonii strain C30,and the non-saccharomyces cerevisiae is a wild non-saccharomyces cerevisiae isolated from Guinong No. 5 prickly pear. According to the present invention, the non-saccharomyces cerevisiae and saccharomyces cerevisiae are adopted for mixed fermentation, and the saccharomyces cerevisiae consumes sugar to produce alcohol; and the non-saccharomyces cerevisiae has low production of alcohol and high production of aroma substances, and the combination of the two yeasts not only ensures the alcohol content of fruit wine, but also improves the type, content and aroma characteristics of aroma substancesin fruit wine.

The invention discloses a composite aroma-producing microorganism as well as a preparation method and application thereof. The composite aroma-producing microorganism is formed by compounding bacillus subtilis, trichoderma reesei, aspergillus niger and hansenula polymorpha at a wet thallus mass ratio of 5:2:1:1. The composite aroma-producing microorganism disclosed by the invention can be used for preparing a filter material for a cigarette filter and greatly improving the aroma and taste of the cigarettes, and has good application value in the cigarette processing production.

The invention provides a hansenula polymorpha expression system, a hansenula polymorpha construction method and application of hansenula polymorpha. The expression system contains uracil auxotroph hansenula polymorpha AU-0501, of which the preservation number is CGMCC NO.7013. The uracil auxotroph hansenula polymorpha AU-0501 provided by the invention has the advantages of definite mutation site, low reverse mutation frequency, good hereditary stability, high biological expression quantity and the like, plays a significant role in researching and producing gene engineering vaccine, has the advantages of higher yield and low cost as compared with other eukaryotic expression systems adopted at present. The invention further provides an expression vector applied to the hansenula polymorpha expression system and a construction method of the expression vector. Two or more genes can be expressed simultaneously through the expression vector. Two target genes can be expressed in a hansenula polymorpha auxotroph cell at a high level without interference.

The invention relates to a mixed fermentation process based on hanseniaspora uvarum and saccharomyces cerevisiae and belongs to the technical field of microorganisms. The mixed fermentation process is characterized in that hanseniaspora uvarum and saccharomyces cerevisiae are subjected to mixed fermentation; the hanseniaspora uvarum is a hanseniaspora uvarum strain QTX22; and the preservation number of the hanseniaspora uvarum strain QTX22 is CCTCC (China Center for Type Culture Collection) M 2021083. Compared with a single-bacterium fermentation product, the mixed-bacterium fermentation product disclosed by the invention has more unique fragrance, and a wine drink with unique flavor, fragrance and mouth feel can be produced, so that consumer goods are enriched, and consumption choices are expanded.

The present invention discloses a geraniol-producing hanseniaspora uvarum and a fermentation method thereof, and belongs to the technical field of fermentation engineering. The hanseniaspora uvarum A14 CGMCC No.18666 has high geraniol-producing capacity, concentration of geraniol can reach 63.48 [mu]g / L after 48 h of fermentation, while the highest content of the geraniol produced by dominant yeasts in other baijiu fermentation processes is only 43.59 [mu]g / L; and during a baijiu brewing process, the hanseniaspora uvarum A14 CGMCC No.18666-containing fungus agent is added, which can significantly increase content of the geraniol in baijiu and also improve flavor and nutritional value of the baijiu.

The present invention relates to a process for producing a human-type glycoprotein having reduced glycosylation by genetically manipulating an enzyme involved in glycosylation using a Hansenula polymorpha system. In detail, the present invention relates to a process for producing a human-type glycoprotein by identifying a dolichyl-phosphate-mannose dependent ¥á-1,3-mannosyltransferase gene from H. polymorpha, constructing a H. polymorpha mutant strain producing a glycoprotein exhibiting reduced glycosylation by disrupting the identified gene, and subjecting the mutant strain to various genetic manipulations for the synthesis of human-type glycan.

The invention discloses construction of recombinant eukaryotic hansenula engineering bacterial strains containing medical hirudin genes and a production process of recombinant hirudin, wherein hansenula polytype host cell strains RB-11 are adopted as initial bacterial strains for constructing hansenula high-expression engineering bacterial strains RBHV 145-6 containing hirudin, and the hirudin with high purity and high content is obtained through culture and fermentation. In the invention, the hirudin genes are copied onto host cell chromosomes in a multi-copy mode, in addition, the copying number is kept stable, exogenous gene expression boxes containing the hirudin genes are completely integrated onto the host cell chromosomes, the existence of free plasmids in seed bank cells and passage 40 and 80 generation cells is avoided, the expression quantity and the secretion efficiency of foreign protein are 10 times higher than those of saccharomyces cerevisiae, particularly through beingcompared with that of the polytypic hansenula in China, the expression quantity of the foreign protein is high and is 35.8 percent, and in addition, the excessive glycosylation phenomenon does not exist, so the production process is suitable for target protein through large-scale fermentation production.

The invention discloses a microbial compound inoculant and a preparation method and application thereof. The inoculant is prepared by mixing mould and yeast according to a weight ratio of (3-5):1. Themould is formed by mixing Aspergillus oryzae and Rhizopus oryzae according to a weight ratio of 1:(1-3); and the yeast is formed by mixing Saccharomyces cerevisiae, Hansenula anomala abnormal variant, Hansenula anomala and Wickerhamomyces anomalus according to a weight ratio of 1:(0.5-1.5):(0.5-1.5):(0.5-1.5). The fen-flavor liquor brewed by the microbial compound inoculant is pure in fen flavor,mellow, sweet, soft, natural, harmonious, refreshing in aftertaste, and high in liquor yield and high-quality liquor yield.

The invention discloses a hanseniaspora uvarum strain QTX22 capable of producing aroma substances at high yield and application thereof, and belongs to the technical field of microorganisms. The preservation number of the hanseniaspora uvarum strain QTX22 is CCTCCM 2021083. The yield of n-hexanol generated by fermenting grape juice by the strain is up to 461.27 [mu] g / L, and the yield of phenethyl acetate is up to 359.4 [mu] g / L.

The invention discloses a method for producing 1,3-propanediol and 2,3-butanediol from raw starch materials, including the following steps: 1) Candida krusei or Hansenula Arabitolgens Fang are inoculated into a fermentation medium with the saccharifying liquid of the raw starches as a carbon source; the yeast cells are cultured on an aerobic condition until glucose-consuming-rate is significantly reduced, and then fermented anaerobically to a glucose concentration from 5 to 10 g / L; the fermentation broth is collected and filtered to remove the yeast cells in the broth, and the resultant filtrate is glycerin fermentation broth; 2) Klebsiella, Clostridium butyricum, or Clostridium pasteurianum are inoculated into a fermentation medium in which the glycerin fermentation broth obtained from step 1) serves as a carbon source; the bacteria are fermented anaerobically for 30-32 hours, and then fermented aerobically when the production rate of 1,3-propanediol decreased obviously, and the fermentation was stopped when the concentration of glycerin is reduced to a level below 10 g / L, and finally 1,3-propanediol and 2,3-butanediol are obtained. The method of the present invention can effectively reduce production cost and increase productivity.

The invention discloses hansenula polymorpha with a double selection marker and an application thereof. The hansenula polymorpha with a double selection marker provided by the invention is uracil-synthesized and tryptophan-synthesized double-deficient hansenula polymorpha HUT-31 with a preservation number of CGMCC No.4778. The strain has a uracil and tryptophan double-auxotrophic selection marker, maintains the physiological biochemical characteristics of wild-type strains, facilitates the culture of recombinant strains and the high-level expression of heterologous protein; not only recombinant strains can be selected easily and rapidly, but also the double selection marker can increase the copy number of target genes integrated on a chromosome, can increase the expression level of targetprotein, and has important industrial application value. Meanwhile, the double selection marker is also applicable to the polygene genetic modification of hansenula polymorpha in functional genomics,synthetic biology, and metabolic engineering.

An expression of recombinant SARS virus gene in polymorphic Hanson yeast and its application are disclosed. In the procedure of industrially preparing the expressed product of SARS virus' genes S, S1and S2 and primary antigen epitope gene, the SARS virus' genes S, S1 and S2 and primary antigen epitope gene are redesigned according to the application method of the codon of high-expression gene for Hanson yeast. The high-expression product can be used as vaccine to prevent the atypical pneumatonitis caused by SARS virus. The designed gene can also be used for fusion expression with cholera toxin B subunit gene in Hanson yeast.

The invention relates to a method for producing HPV45 L1 protein by using a Hansenula polymorpha expression system. Specifically, the invention discloses a method for producing a recombinant Hansenula polymorpha cell expressing the HPV45 L1 protein and the recombinant Hansenula polymorpha cell produced by using the method. The invention further discloses a method for producing the HPV45 L1 protein by using the recombinant Hansenula polymorpha cell and application of the produced HPV45 L1 protein in preparation of prophylactic vaccines.

The invention discloses a composite microbial deodorizer and a preparation method thereof. According to the invention, thiobacillus ferrooxidans, rhizobium sludge, bacillus amyloliquefaciens, rhodopseudomonas palustris, pseudomonas stutzeri, bacillus coagulans and hansenula polymorpha are selected for use; liquid fermentation is performed respectively, and then compounding is performed in proportion; then linalool is added in proportion and the substances are fully mixed to prepare a composite microbial deodorizer capable of effectively expelling flies and insects, inhibiting growth and propagation of harmful flora and reducing and removing foul gas in the environment. The thiobacillus ferrooxidans (CFU / g) in the product is greater than or equal to 2.95*10<8>; the rhizobium sludge (CFU / g)is greater than or equal to 1.05*10<8>; the bacillus amyloliquefaciens (CFU / g) is greater than or equal to 1.73*10<8>; the rhodopseudomonas palustris (CFU / g) is larger than or equal to 2.55*10<8>; thepseudomonas stutzeri (CFU / g) is larger than or equal to 1.15*10<8>; the bacillus coagulans (CFU / g) is larger than or equal to 2.86*10<8>; the hansenula anomala (CFU / g) is larger than or equal to 2.97*10<8>; and the total amount of organic acids is larger than or equal to 25.8 g / L. The product provided by the invention has an obvious deodorization effect, the NH3 removal rate is greater than or equal to 78.14%, the H2S removal rate is greater than or equal to 71.64%, the odor concentration elimination rate is greater than or equal to 84.95%, and the mosquito and fly expelling rate is greater than or equal to 74.16%.

The invention relates to a method for producing HPV31 L1 protein by using a Hansenula polymorpha expression system. Specifically, the invention discloses a method for producing a recombinant Hansenula polymorpha cell expressing the HPV31 L1 protein and the recombinant Hansenula polymorpha cell produced by using the method. The invention further discloses a method for producing the HPV31 L1 protein by using the recombinant Hansenula polymorpha cell and application of the produced HPV31 L1 protein in preparation of prophylactic vaccines.

The invention provides a hansenula polymorpha recombinant strain and an application thereof in biosynthesis of paclitaxel. A total DNA transformation yeast of mediated taxus chinensis genome is injected to obtain a hansenula polymorpha recombinant strain CGMCC No.8999 for producing paclitaxel by using low-energy ions. The technical breakthrough for biological fermentation to produce paclitaxel by using the recombined yeast is achieved, and a new way is provided for solving paclitaxel source shortage, protecting taxus chinensis plant resources in China and sustainable development thereof.

The invention relates to Hansenula polymorpha for preparing high-lysine single-cell protein through methyl alcohol and application of the Hansenula polymorpha. The classification name of the Hansenula polymorpha is Hansenula polymorpha AP23, the Hansenula polymorpha is preserved in the China Center for Type Culture Collection (CCTCC) on April 5th, 2016, and the preservation number is CCTCC No: M 2016173. By means of the plasma induced mutation technology, the strain capable of producing high-lysine single-cell protein is screened out with methyl alcohol as the only carbon source, and on this basis, the Hansenula polymorpha for efficiently utilizing methyl alcohol is screened out in a gradient mode through the further combination of methyl alcohol. By means of the strain, high-density fermentation can be achieved, methyl alcohol protein production is conducted through a 5 L fermentation tank, the final bacterium wet weight reaches 350-400 g / L, the methyl alcohol protein dry weight reaches 115-130 g / L, the protein content reaches 68%, and the lysine content reaches up to 60 mg / g. The Hansenula polymorpha has great social significance and economic value in development of animal feed protein additives.

The invention provides a standard plasmid for detecting the copy number of an exogenous gene of a recombinant CVA10 vaccine as well as a preparation method and application of the standard plasmid. The standard plasmid is formed by connecting an endogenous gene MOX fragment SEQ ID No.1, an exogenous gene P1 fragment SEQ ID No.2 and an exogenous gene 3CD fragment SEQ ID No.3 in series in a cloning vector. According to the method, the copy number of the inserted exogenous gene is simply, conveniently, rapidly, absolutely quantitatively and accurately analyzed by constructing the standard plasmid and adopting a Taqman probe-fluorescent quantitative PCR method, so that the detection time for determining the copy number of the exogenous gene of the recombinant CVA10 vaccine is effectively shortened, repeated operation is facilitated, and the detection efficiency is improved. The method has a wide application prospect in the determination of the copy number of the recombinant CVA10 vaccine exogenous gene constructed based on a hansenula polymorpha platform and the verification of the genetic stability of the recombinant CVA10 vaccine exogenous gene.

The invention relates to a method for generation of HPV58 L1 (human papillomavirus) protein by a Hansenula expression system. Specifically, the invention discloses a method for generation of a recombinant Hansenula cell for expressing the HPV58L1 protein and the recombinant Hansenula cell generated thereby. The invention also discloses a method for generating the HPV58L1 protein by the recombinant Hansenula cell and application of the generated HPV58L1 protein in preparation of preventive vaccines.

The invention provides a hansenula polymorpha-based method for producing D-arabitol. The method comprises the following steps: carrying out fermented seed cultivation on hansenula polymorpha after domestication under a hyperosmosis condition, and then inoculating into a fermentation medium; firstly, cultivating at 30-38 DEG C for 18-24 hours, and then continuing to cultivate at 40-49 DEG C for 18-24 hours; and finally continuing to cultivate at 28-38 DEG C for 74-96 hours. The ability of the hansenula polymorpha for producing the D-arabitol is improved by comprehensive strain domestication and fermentation temperature regulation technologies, and the dry cell weight and the yield of the D-arabitol respectively can achieve 5.26g / L and 114.92g / L. According to the method, the yield of the D-arabitol produced by hansenula polymorpha fermentation can be increased; in addition, the method provided by the invention is easy to operate, relatively low in cost, and easy to apply in the fermentation method for producing the D-arabitol.

The invention relates to a method for producing HPV31 L1 protein by using a hansenula polymorpha expression system. Specifically, the invention discloses a method for producing recombinant hansenula polymorpha cells expressing the HPV31 L1 protein and the recombinant hansenula polymorpha cells produced by the method. The invention also discloses a method for producing the HPV31 L1 protein by usingthe recombinant hansenula polymorpha cells and application of the produced HPV31 L1 protein in preparation of a preventive vaccine.

The invention discloses an artificial mixed starter culture for highland barley wine and application thereof in fermentation of the highland barley wine. The artificial mixed starter culture for the highland barley wine comprises rhizopus oryzae, aspergillus japonicus, saccharomyces cerevisiae and hansenula anomala, or further comprises actinomucor elegans on the basis of the rhizopus oryzae and the saccharomyces cerevisiae. All the raw materials are compounded to prepare the artificial mixed starter culture for the highland barley wine. The artificial mixed starter culture for the highland barley wine solves the problems that the existing starter culture for the highland barley wine has various microorganisms and uncontrollable varieties and the like, and can provide reference for promoting the industrial production of the highland barley wine under an artificially controllable condition.

The invention discloses a saccharomyces cerevisiae strain and a Wickerhansenula anomala strain, and applications of the saccharomyces cerevisiae strain and the Wickerhansenula anomala strain in improvement of color stability of fruit wine. The two strains of saccharomyces cerevisiae are preserved in the China General Microbiological Culture Collection Center, the preservation number of the saccharomyces cerevisiae is CGMCC (China General Microbiological Culture Collection Center) No.22751, and the preservation number of the Wickerhasenula anomala is CGMCC No.22753. Through detection, the two yeasts have excellent hydroxycinnamic acid decarboxylase activity; the grape wine, the blueberry wine, the mulberry wine and the cherry wine which are rich in anthocyanin such as cyanidin-3-O-glucoside, cyanidin-3-O-rutinoside, malvidin-3-O-glucoside, pelargonidin-3-O-glucoside, delphinidin-3-O-glucoside and the like can be added; the content of pyran-type anthocyanin derivatives in the fermentation and ageing process of fruit wine such as blueberry wine is increased, so that the color stability of the aged fruit wine is enhanced, and the sensory quality is improved.

The invention discloses a strain of saccharomyces cerevisiae and a strain of hansenula vinosa, and application of the two strains of wild yeast in reducing the content of biogenic amine in fruit wine. The two strains of yeast are preserved in the China General Microbiological Culture Collection Center, wherein the preservation number of the saccharomyces cerevisiae is CGMCC No. 2350523505 and the preservation number of the hansenula vinosa is CGMCC No. 23504. Through detection, the two strains of yeast have relatively strong biogenic amine degradation capability, and can reduce the content of tryptamine, beta-phenylethylamine, putrescine, cadaverine, histamine, tyramine, spermidine and spermine in a fermentation process of the fruit wine such as grape wine, blueberry wine, mulberry wine, indigo wine, cherry wine, bilberry wine, red raspberry wine, hawthorn wine and strawberry wine.

The invention belongs to the technical field of microbial preparations, in particular to a hanseniaspora opuntiae culture method and an application thereof. The invention provides a hanseniaspora opuntiae culture method and an application thereof. The culture method adopts solid culture and has lower equipment requirements; and the cost of raw materials is low, with the production cost of one ton being lower than 200 yuan. The hanseniaspora opuntiae culture cultured by the method can effectively reduce the release amount of malodorous gases such as ammonia, hydrogen sulfide and the like in livestock and poultry excrement; and in addition, the hanseniaspora opuntiae culture can produce pleasant fragrance and play the role of masking odor when decomposing odor components.

The invention discloses a geraniol-producing grape juice Hansenula sporogenes and a fermentation method thereof, belonging to the technical field of fermentation engineering. The grape juice Hansenia spora uvarum (Hanseniaspora uvarum) A14 CGMCC No.18666 of the present invention has a high ability to produce geraniol, and the concentration of geraniol after 48 hours of fermentation can reach 63.48μg / L, while other dominant yeasts in the liquor fermentation process The highest content of geraniol produced was only 43.59 μg / L; adding the bacterial agent containing Hansenia spora uvarum (Hanseniaspora uvarum) A14 CGMCC No.18666 in the process of brewing liquor can significantly increase the geraniol content in liquor content, and enhance the flavor and nutritional value of liquor.

The invention discloses a flavored fermented rice cake and a preparation method thereof. The flavored fermented rice cake is prepared by using rice as a raw material, as well as adopting a multi-bacterium co-fermentation technology involving lactobacillus plantarum, saccharomyces cerevisiae, hanseniaspora uvarum and saccharomycopsis fibuligera in combination with a micro-capsule embedding technology. The preparation method of the flavored fermented rice cake comprises the following steps: (1) preparing a compound fermentation agent; (2) preparing powder; (3) carrying out first fermentation; (4) carrying out aroma-improving fermentation; (5) carrying out micro-capsule embedding; (6) carrying out mixed second fermentation so as to obtain a fermented product; (7) filling a mold with the fermented product, and carrying out leavening; and (8) carrying out packaging. The flavored fermented rice cake prepared by the preparation method is strong in layered taste, mellow and coordinated in flavor, long-lasting in aroma, and resistant to storage.