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Hanseniaspora opuntiae culture method and microbial deodorization method for livestock and poultry excrement

A technology of Hansenula yeast and microorganism deodorization, applied in microorganism-based methods, microorganisms, biological sludge treatment and other directions, can solve problems such as poor deodorization effect and high production cost, achieve low equipment requirements, mask odor, etc. The effect of taste and low cost of cultivation

Inactive Publication Date: 2020-02-28
咸阳润源生物科技有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the deodorization effect of these strains is not good, and the production and cultivation of bacterial strains generally adopt liquid culture, and the required equipment (such as fermenter) and the composition of the medium will cause higher production costs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Cultivation method of Hansenula spp.:

[0036] (1) Select sugarcane peel, pineapple peel and ginkgo biloba residue as raw materials, all of which have a moisture content of 15%, crush them into about 0.1cm in size, weigh 2000, 2000 and 3000g respectively, and mix them in a mass ratio of 2:2:3 Mix well, add 2100mL water to obtain a solid medium, and sterilize by steaming at 100°C for 45min;

[0037] (2) Inoculate the slanted seeds of Hansenula spp. in cactus in a molasses liquid medium with a sugar concentration of 2%, and vibrate on a shaker at 28° C. for 16 hours;

[0038] (3) Take the cultured liquid seeds, inoculate them on the sterilized solid medium, mix well, the thickness of the medium is 10cm, control the ambient temperature at 28°C, and cultivate for 48h. Spray a small amount of sterile water to finally get the yeast culture;

[0039] (4) Calculated by plate viable bacteria, the number of effective viable bacteria is 2.2×10 9 CFU / g.

Embodiment 2

[0041] Cultivation method of Hansenula spp.:

[0042] (1) Select sugarcane peel, pineapple peel and ginkgo leaf pomace as raw materials, the water content of which is 15%, and be crushed into about 0.2cm in size, weigh 4000, 4000 and 5000g respectively, and mix them in a mass ratio of 4:4:5 Evenly, add 4550mL of water to make the final moisture content 50%, to obtain a solid medium, steam and sterilize at 100°C for 50min;

[0043] (2) Inoculate the slanted seeds of Hansenula spp. in cactus in a molasses liquid medium with a sugar concentration of 3%, and vibrate on a shaker at 28° C. for 18 hours;

[0044] (3) Take the cultured liquid seeds, inoculate them on the sterilized solid medium, mix well, the thickness of the medium is 12cm, control the ambient temperature at 30°C, and cultivate for 54h, during which the material is turned every 12h, and the material layer The surface is sprayed with a small amount of sterile water, and finally the yeast culture is obtained;

[0045...

Embodiment 3

[0047] Cultivation method of Hansenula spp.:

[0048] (1) Select sugarcane peel, pineapple peel and ginkgo leaf pomace raw material, its moisture content is 15%, be crushed into about 0.4cm sized powder, weigh 5000, 5000, 6000g respectively, mix by mass ratio 5:5:6 Mix well, add 6400mL of water to make the final moisture content 55%, obtain a solid medium, and sterilize by steaming at 100°C for 60min;

[0049] (2) inoculate the slanted seeds of Hansenula spp. in cactus in molasses liquid culture medium with a sugar concentration of 4%, and vibrate on a shaker at 28°C for 20h;

[0050] (3) Take the cultured liquid seeds, inoculate them on the sterilized solid medium, mix well, the thickness of the medium is 15cm, the ambient temperature is controlled at 32°C, and cultivated for 60h, during which the material is turned every 12h, and the material layer The surface is sprayed with a small amount of sterile water, and finally the yeast culture is obtained;

[0051] (4) Calculate...

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PUM

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Abstract

The invention belongs to the technical field of microbial preparations, in particular to a hanseniaspora opuntiae culture method and an application thereof. The invention provides a hanseniaspora opuntiae culture method and an application thereof. The culture method adopts solid culture and has lower equipment requirements; and the cost of raw materials is low, with the production cost of one ton being lower than 200 yuan. The hanseniaspora opuntiae culture cultured by the method can effectively reduce the release amount of malodorous gases such as ammonia, hydrogen sulfide and the like in livestock and poultry excrement; and in addition, the hanseniaspora opuntiae culture can produce pleasant fragrance and play the role of masking odor when decomposing odor components.

Description

technical field [0001] The invention belongs to the technical field of microbial preparations, and in particular relates to a method for cultivating Hansenula spp. and a microbial deodorization method for livestock and poultry manure. Background technique [0002] The large-scale and intensive development of the livestock and poultry breeding industry, while promoting the development of the agricultural economy, has also brought serious environmental pollution problems. The environment had a bad influence. Odor components in livestock and poultry manure include: volatile sulfur compounds, nitrogen compounds, indoles and phenols, and volatile fatty acids, which mainly come from the catabolism of certain components in manure by microorganisms in manure. [0003] At present, there are three main types of deodorization of livestock and poultry manure: physical method, chemical method and biological method. The physical method refers to the use of adsorbents and masking agents ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C02F11/02C02F103/20C12R1/78
CPCC12N1/16C02F11/02C02F2103/20C02F2303/02
Inventor 武兴友武春锋
Owner 咸阳润源生物科技有限公司
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