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Immune colloidal gold chromatography method for detecting food allergy to aquatic products

A technology for food allergy and colloidal gold, which is used in material inspection products, measuring devices, biological tests, etc., to achieve the effects of clear and storable results, low cost, and easy operation.

Inactive Publication Date: 2009-02-04
JIMEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Food Industry Science and Technology, 2007, 12: 216-218), but there is no immune colloidal gold chromatography detection method for crustacean tropomyosin (TM) and fish parvalbumin (Par) food allergy

Method used

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  • Immune colloidal gold chromatography method for detecting food allergy to aquatic products
  • Immune colloidal gold chromatography method for detecting food allergy to aquatic products
  • Immune colloidal gold chromatography method for detecting food allergy to aquatic products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: Colloidal gold chromatography of rabbit anti-TM antibody detects whether TM is contained in the sample

[0024] (1) Preparation of colloidal gold. Take 1.0mL of chloroauric acid and add it to 100mL of ultrapure water. After heating to boiling, quickly add 4mL of 1% trisodium citrate, heat and boil for 8 minutes to obtain a transparent, wine-red colloidal gold solution.

[0025] (2) Colloidal gold labeling of proteins. Add rabbit anti-TM antibody to the colloidal gold solution drop by drop while stirring, the final protein concentration is about 8 μg / mL, continue to stir for 0.5 h; add 200 μL 10% bovine serum albumin (BSA) drop by drop while stirring, continue to stir for 10 min , let stand overnight. Centrifuge the protein solution labeled with colloidal gold at 5000g at 4°C for 10min; take the supernatant and centrifuge at 17000g at 4°C for 30min; suck out the supernatant to obtain colloidal gold-labeled protein, and dissolve the precipitate in 2mL of p...

Embodiment 2

[0030] Example 2: Colloidal gold chromatography of shrimp and crab TM detects whether TM antibody is contained in the serum of allergic persons

[0031] Such as Figure 4 Shown, label 1. Shrimp TM; 2. Scylla TM; 3. Colloidal gold-labeled prawn TM.

[0032] The preparation of the immunocolloidal gold chromatography test strip refers to Example 1 (wherein the colloidal gold-labeled protein is replaced by the shrimp and crab TM in this example from the rabbit anti-TM antibody in Example 1). Immobilize 1 μL of Shrimp TM and Scylla TM on the two detection areas on the nitrocellulose membrane of the immunogold test strip, and add 12 μL of colloidal gold-labeled Shrimp TM to the gold label area to detect whether there are TM antibodies in human serum samples. From Figure 4 It can be seen from the note A in the test that the sample to be tested is the serum of a person allergic to TM, and as a result, red spots appear in both detection areas; Figure 4 The sample to be tested in pap...

Embodiment 3

[0033] Example 3: Colloidal gold chromatography of silver carp Par detects whether there is Par antibody in the serum of allergic persons

[0034] Such as Figure 5 As shown, labels 1. Silver carp Par; 2. Crucian carp Par; 3. Colloidal gold-labeled silver carp Par.

[0035] The preparation of the immunocolloidal gold chromatography test strip refers to Example 1 (wherein the colloidal gold-labeled protein is replaced by the silver carp Par in this example from the rabbit anti-TM antibody in Example 1). 1 μL silver carp Par and crucian carp Par were immobilized in the two detection areas on the nitrocellulose membrane, and 12 μL colloidal gold-labeled silver carp Par was added to the gold label area to detect whether Par antibodies were contained in human serum samples. From Figure 5 It can be seen from the paper note A in the test that the sample to be tested is the serum of a person allergic to Par, and red spots appear in both detection areas; Figure 5 The sample to be ...

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Abstract

The present invention discloses a gold-immunochromatography assay method which is used for detecting the aquatic product food allergy. The method comprises preparing colloidal gold, labeling protein with the colloidal gold, detecting with the gold-immunochromatography assay, and other steps. The invention adopts the gold-immunochromatography assay technology to detect the aquatic product food allergy; the technology not only has the advantages of high specificity and high sensitivity of the enzyme immunoassay, but also has the advantages of rapid and simple detection of the prick test; and the technology can rapidly detect whether the food has aquatic product allergens or aquatic product allergen antibodies.

Description

technical field [0001] The present invention relates to the detection method of food allergy, particularly relate to a kind of immune colloidal gold chromatography method that detects aquatic product food allergy, and this method is mainly aimed at crustacean tropomyosin (TM) and fish parvalbumin (Par) Immunocolloidal gold chromatography detection of food allergy. Background technique [0002] Food allergy is also called allergic reaction in medicine. Substances that cause food allergic reactions are called food allergens, such as fish, shrimp, crab, milk, eggs, etc. The reaction mechanism is that after the allergen enters the body, it will cause the body to have a normal or excessive immune response, that is, a hypersensitivity reaction. When exposed to the same allergen again, multiple IgE molecules bind to the antibody, causing changes in the structure of the Fc end of IgE, leading to cell degranulation, releasing histamine, serotonin, leukotrienes and other biological ...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/543
Inventor 刘光明曹敏杰沈苑
Owner JIMEI UNIV
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