124 results about "Desulfobacter sp." patented technology

The invention relates to a preparation method and application of nutrient source immobilized sulfate reducing bacterium globules. Immobilized globules take polyvinyl alcohol and sodium alginate as carriers, a saturated boric acid calcium chloride solution as a cross-linking agent and sodium lactate as a nutrient source. The preparation method comprises the specific steps: firstly, dissolving the polyvinyl alcohol and the sodium alginate in a water bath at a constant temperature; then, adding active carbon, calcium carbonate, silicon dioxide and free bacterium liquid; sucking a mixed solution by an injector and dropwise adding the mixed solution into the saturated boric acid calcium chloride solution; crossly linking for 24h to form the globules; washing the globules with sterile physiological saline and putting the globules into a sodium lactate solution for 24h; after washing, freezing and drying to obtain the nutrient source immobilized sulfate reducing bacterium globules. In the application of the nutrient source immobilized sulfate reducing bacterium globules prepared by the invention, the nutrient source immobilized sulfate reducing bacterium globules have a good removing effect on four types of heavy metal including Cu, Zn, Pb and Cd in sediment of Xiang River; the treatment is stable and the leaching rate is low. The material is convenient to separate, can be repeatedly utilized and does not cause secondary pollution; the nutrient source immobilized sulfate reducing bacterium globules has good economic benefits and a wide application prospect.

The invention relates to a removal method of heavy metal ion in wastewater, such as chrome, belonging to the technical field of wastewater treatment. The removal method is characterized in that nanometer FeSx is produced by culturing a compound bacteria comprising desulfovibrio Sp. CB1. 268, desulfotomaculum Sp. CB1.139, and desulfobacter Sp. CB1.168 in a culture medium comprising lactic acid proteinate, ferrite, sulfate, K, Mg, Ca, Cu, Mn, B, Si, and Mo under anaerobic or facultative, and alkaline condition at the temperature between 30 and 39 DEG C for thirty-six to 72 hours; the nanometer FeSx is dissociated into S<2-> and Fe<2+> under acidic condition, can reduce Cr<6+> to Cr<3+>, and generate unsolvable sedimentation of metal sulphides with Ni<2+>, Cu<2+>, Zn<2+>, and other elements; after the sedimentation is separated, the metal ions in the wastewater are removed. The removal method has the advantages of high removal efficiency and low cost.

The invention relates to the technical field of sewage treatment agents, and relates to an oil field water biological treatment agent as well as a preparation method and a use method thereof. The oil field water biological treatment agent is obtained according to the preparation method which comprises the following step: sufficiently mixing a required quantity of denitrification and nitrification bacteria solutions, nitrate, nitrite, biquaternary ammonium salt sludge strippers, oil field sewage, potassium permanganate, potassium dichromate and water to obtain the oil field water biological treatment agent. The oil field water biological treatment agent disclosed by the invention can sufficiently exert the richness and effectiveness of indigenous beneficial microorganisms by being prepared by adopting oil field sewage, comprehensively inhibit the activity and growth of sulphate reducing bacteria and control the generation of hydrogen sulfide by enabling the indigenous beneficial microorganisms to compete with the residual sulphate reducing bacteria by virtue of a higher advantage, fundamentally reduce the problem of secondary hydrogen sulfide caused by the sulphate reducing bacteria by inhibiting the growing environment of the sulphate reducing bacteria and changing the generation way of corrosion, prevent the factor of increasing oil field corrosion and inhibit the cost.

The invention discloses a method for cooperatively treating arsenic-containing waste liquid by using sulfate reducing bacteria and lead-zinc smelting slag. The method comprises the following steps: (1) stirring and mixing the lead-zinc smelting slag and the arsenic-containing waste liquid in an anaerobic environment and conducting reacting to obtain to-be-treated waste liquid; and (2) adding sulfate reducing bacteria into the to-be-treated waste liquid obtained in the step (1), adding sodium lactate, and carrying out microbiological treatment, so as to synergistically treat the arsenic-containing waste liquid by the sulfate reducing bacteria and the lead-zinc smelting slag. According to the method for cooperatively treating the arsenic-containing waste liquid through the sulfate reducing bacteria and the lead-zinc smelting slag, the sulfate reducing bacteria and the lead-zinc smelting slag cooperate with each other, the removal rate of arsenic in the arsenic-containing waste liquid is jointly increased, and the removal rate of arsenic in the arsenic-containing waste liquid reaches 99.80% or above.

The invention relates to a sulfate reducing bacteria microbe corrosion method of magnesium alloy, for resolving the microbe corrosion problem of magnesium alloy and combining the microbe cultivation technique and microbe corrosion technique of solid culture medium to provide a novel magnesium alloy microbe corrosion method based on solid culture medium. The method comprises (1) processing and disinfecting sample, (2) arranging and disinfecting a solid culture medium, (3) cultivating and activating bacterial, (4) corroding paster. The invention optimizes the components of electrolyte to highlight the bacterial performance in the corrosion process of magnesium alloy, and compares the electrode surface corrosion morphology with bacterial and without bacterial, to provide the reference for magnesium alloy microbe corrosion mechanism research. The inventive method is suitable for magnesium alloy microbe corrosion research.

The invention provides biological activated lignite synergistic sulfate reducing bacteria sludge immobilized particles and a preparation method and application thereof. The immobilized particles can be used for reducing, adsorbing and removing various metal cations and anions in acid mine wastewater, and the preparation method of the immobilized particles comprises the following steps: 1) polyvinyl alcohol and sodium alginate are added into water, and swelling, heating and stirring are conducted until bubble-free gel is formed; 2) lignite is added into the gel obtained in the step 1), and uniform stirring and cooling are conducted; 3) rhodopseudomonas sphaeroides liquid and sulfate reducing bacteria sludge are added into the gel mixture obtained in the step 2), the mixture is uniformly stirred, and particles are prepared from the uniformly stirred mixture; 4) the particles obtained in the step 3) are dropwise added into a CaCl2-containing saturated boric acid aqueous solution with the pH value of 5.0-7.0, and crosslinking is carried out under stirring; and then normal saline is used for washing, and moisture on the surface is adsorbed to obtain the biological activated lignite synergistic sulfate reducing bacteria sludge immobilized particles.

The invention discloses a device for in-situ remediation of cadmium and lead contaminated farmland soil by microbial electrochemical enhanced sulfate reduction and a method thereof. According to the method, a sulfate reduction reactor is used for generating the sulfur-containing (S<2->) water system passivator, the passivator can be used for in-situ remediation of cadmium and lead polluted farmland soil, and cadmium and lead are converted into forms which are difficult to absorb by crops. A double-chamber sulfate reduction reactor is constructed, current is applied to promote hydrogen production reaction of a cathode chamber, and autotrophic sulfate reducing bacteria in the cathode chamber can reduce sulfate into S <2-> by utilizing hydrogen and an inorganic carbon source generated by water electrolysis of an electrode. After the passivator containing S<2-> is mixed with soil, the toxicity of cadmium and lead in farmland soil can be reduced. The liquid passivator is used for restoringthe cadmium and lead polluted farmland soil, the physicochemical property of the soil cannot be obviously changed, and the device and the method have obvious advantages compared with the mode of restoring the heavy metal polluted soil through quick lime, biochar and the like.

The invention discloses a gas stripping internal circulation type treatment process and a gas stripping internal circulation type treatment system for treating acid mine wastewater by utilizing SRB. The process comprises the steps of stripping a reaction zone to a reduction atmosphere, carrying out a biochemical reduction sulfur production reaction, carrying out a first precipitation reaction andcarrying out a second precipitation reaction; the system comprises a first precipitation reaction device, a second precipitation reaction device, a gas stripping reaction device and a nutrient solution storage device. According to the method, the influence of multiple (acid, heavy metal and sulfide) toxicity inhibition on the sulfate reduction efficiency can be avoided; in addition, sulfate reducing bacteria in the gas stripping reaction device are high in activity and quick in alkali production, the pH of the acid mine wastewater in the second precipitation reaction device can be directly neutralized to be neutral, pretreatment of the acid mine wastewater is not needed, and the cost is reduced; the sulfate reduction rate in the gas stripping reaction device is high, the sulfide yield is high, heavy metal ions in the acid mine wastewater in the precipitation reaction device can be rapidly removed, and meanwhile valuable metal is recycled.

The invention discloses a remediation method for remediation of antimony and zinc combined pollution and application, and particularly relates to the field of soil remediation. The remediation method comprises the steps of enrichment of sulfate reducing bacteria, curing of the sulfate reducing flora on the Sb (III) and the Zn (II) and acute toxicity detection. When the microbial method provided by the invention is used for treating antimony-zinc combined pollution, the cost is low, the efficiency is high, and harmful pollutants in the environment can be treated in situ, so that the sustainable development of the environment is realized. The microbial metabolism capacity is high, the specific surface area is large, and the surface is charged, so that the microbial can repair heavy metal pollution through cell metabolism, biological adsorption, precipitation and redox. The sulfate reducing flora provided by the invention can be used for simultaneously converting Sb (III) and Zn (II) into sulfide precipitates and converting free forms of the Sb (III) and the Zn (II) into insoluble precipitates, so that the environmental pollution degree is reduced, a bioremediation material is provided for remediation of heavy metal pollution of mines, and the sulfate reducing flora has a very good industrial application prospect.

The invention relates to a method of preparing various different fluorescent Znx-Cd1-xS quantum dots having uniform particle size simply and efficiently in batch through zinc-cadmium wastewater via sulfate-reducing bacteria, and belongs to the technical field of green techniques. Quantum dots about 7 nm in uniform particle size and having good dispersity are prepared by adding sulfate-reducing bacteria; various Znx-Cd1-xS quantum dots with different light-emitting peaks are prepared according to wastewaters having different Zn-Cd concentration ratios. When the Zn-Cd concentration ratio in a solution is 3:1 to 20:1, the prepared ZnxCd1-xS quantum dots have photoluminescent spectrum from red light to blue light when excited at 365 nm, and have photoluminescent peaks at 470-590 nm. The different fluorescent ZnxCd1-xS ternary quantum dots are synthesized for the first time herein through a microbiological method; the problems of the prior art, such as high zinc-cadmium wastewater yield, high toxicity and low recycling value, are solved.

The invention provides a method for processing and recycling oil-field exploitation waste water, making air oxidation and catalytic oxidation on the waste water, making catalytic oxidation and air oxidation on volatile phenol, sulfide, Fe2+, etc, on more moderate reaction conditions, and modifying them; filtering out deposits, removing oxygen from the filtrate by low-temperature desorption and obtaining oxygen free water, and finally killing sulfate reducing bacterium, saprophyte and iron bacterium by UV sterilizing processing. And the test shows that it can process polymer-containing waste water in oil field to make the waste water directly reach water reinjection requirements, having low operating expenses.

The invention provides a sludge compost heavy metal passivation microbial agent as well as a production method and application thereof. The passivation microbial agent comprises the following components in parts by weight: 10-16% of thermophilic sulfate reducing bacteria, and 4-6% of bacillus, with the balance being modified charcoal. The modified charcoal comprises 90 to 95 percent of charcoal and 5 to 10 percent of nano silicon dioxide loaded in pore channels of the charcoal; the average pore diameter of the modified charcoal is 6 to 9 nm; the specific surface area is 422-495 m<2>/g. In thesludge compost heavy metal passivation microbial agent obtained by the scheme, the fixation rate of microorganisms is 80-85%, the content of active heavy metal Cr is reduced by 85% or above, the amount of active Cu is reduced by 87% or above, and the amount of active Cd is reduced by 84% or above.

The invention belongs to the technical field of environmental protection, and particularly relates to a thermophilic bio-trickling gas-liquid two-phase device and application thereof in removal of heavy metals in flue gas. The thermophilic bio-trickling gas-liquid two-phase device comprises a thermophilic bio-trickling device unit and an AA/O unit, the thermophilic bio-trickling device is internally provided with a three-stage filler layer (attached with a biological membrane), and high-temperature-resistant special-effect denitrifying bacteria and sulfate reducing bacteria are added to the biological membrane and are used for converting and stabilizing heavy metals in a gas phase. The AA/O unit is composed of an anaerobic tank, an anoxic tank, an aerobic tank and a sedimentation tank, anddenitrifying bacteria, devulcanizing bacteria and sulfurizing bacteria are added to convert and stabilize heavy metals in a liquid phase. The thermophilic bio-trickling gas-liquid two-phase device can be used for removing heavy metals in flue gas. The method for efficiently removing heavy metals in flue gas based on the device can overcome the defects that an adsorbent in an adsorption method inan existing heavy metal flue gas treatment technology is easy to saturate, secondary pollution such as hazardous wastes is generated, and the operation cost is relatively high.

The invention relates to a sulfur autotrophic denitrification advanced denitrogenation device using sulfate in water as a sulfur source. The device comprises a filter tower. Secondary effluent to be treated enters from the lower part of the filter tower, and is treated and then discharged from the upper part of the filter tower. The filter tower is also provided with an exhaust port. The filter tower comprises a sulfate reduction area located at the lower part and a sulfur autotrophic denitrification area located at the upper part. A solid slow-release carbon source feeding hole is formed between the sulfate reduction area and the sulfur autotrophic denitrification area, and a slow-release carbon source can be supplemented to the sulfate reduction area through the solid slow-release carbon source feeding hole. The sulfate reduction area is filled with a filter material, and a sulfate reducing bacteria biological membrane is hung on the filter material. The sulfur autotrophic denitrification area is filled with a filter material, and a sulfur autotrophic denitrifying bacterium biological membrane is hung on the filter material. According to the invention, with sulfate contained in wastewater as an indirect nutrition source, sulfur autotrophic denitrification advanced denitrogenation is carried out under the condition of no additional sulfur source, so that resources are saved, the utilization rate of the sulfur source is maximized, and the operation cost is reduced. The process and the device are especially suitable for treating secondary effluent with low C/N ratio.