The invention discloses a quick high-sensitivity microbiological identification method based on micromolecular metabolic substance
spectral analysis. The micromolecular metabolic substance of
microorganism is served as a detection object of the method, and the
microorganism is identified through combining a
mass spectrometry with a
chemometrics method. The method specifically comprises the following steps: 1, culturing a
monoclonal growing
microorganism strain to be identified; 2, centrifuging an obtained
monoclonal microorganism
thallus sample to be identified to remove a medium, adding water or a
phosphate buffer solution with the pH value of 7.2-7.6 to wash, and centrifuging to remove the residual medium; 3, adding
ethanol water which has the
volume fraction of 75-95% and is 2-4 times of the volume of the
monoclonal microorganism
thallus into the monoclonal microorganism
thallus to be identified, and thus obtaining an extracting solution sample; 4, carrying out
mass spectrometry test on the extracting solution sample; and 5, acquiring 5-20 spectral peaks from a
sample mass spectrogram, analyzing the obtained data through utilizing a
chemometrics method based on multi-variate
statistical analysis, and realizing the thallus identification.