The invention provides a novel multilayer coating system enteric preparation for dexlansoprazole. The preparation comprises a drug layer I, a drug layer II, enteric coating layers IV and isolating layers III, wherein the isolating layers III are arranged among the drug layer I or II and the enteric coating layers IV, the drug layer I and the drug layer II are respectively composed of dexlansoprazole, a filler, a binder, a stabilizing agent and a lubricant, and the preparation comprises, from interior to exterior, the drug layer I, the isolating layer III-1, the enteric coating layer IV-1, the isolating layer III-2, the drug layer II, the isolating layer III-3 and the enteric coating layer IV-2. The novel multilayer coating system enteric preparation for dexlansoprazole provided by the invention can improve the acid inhibition effect of dexlansoprazole and prolongs action time of dexlansoprazole, the isolation effect of the isolating layers in the whole preparation system is optimized, and stability of product quality is ensured; and a preparation method provided by the invention is applicable to industrial production and has a great application value.